Mouse Integrin beta 7 Antibody

Catalog #: AF3060 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3060-SP	Ships in 5-7 Days	25 ug / $149
AF3060	In Stock	100 ug / $449

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Integrin beta 7 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Integrin beta 7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) Integrin beta 1, rmIntegrin beta 2, recombinant human Integrin beta 3, and rmIntegrin beta 6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 7
Glu20-His722
Accession # P26011

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Integrin beta 7

Flow Cytometry

2.5 µg/10⁶ cells

Mouse lymph node single‑cell suspension

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 7

Integrin beta 7 is a 130 kDa type I transmembrane glycoprotein that belongs to the integrin beta chain family. It heterodimerizes with either Integrin alpha 4 or alpha E. Whereas, Integrin alpha E beta 7 is a receptor for E-Cadherin, Integrin alpha 4/ beta 7 is a receptor for fibronectin, MAdCAM 1 and VCAM1. The extracellular domain of mouse Integrin beta 7 shares 94% and 87% amino acid sequence identity with that of rat and human Integrin beta 7, respectively.

Entrez Gene IDs

3695 (Human); 16421 (Mouse); 25713 (Rat); 102137082 (Cynomolgus Monkey)

Alternate Names

Gut homing receptor beta subunit; integrin beta 7 subunit; Integrin beta 7; integrin beta-7; integrin, beta 7; ITGB7