Mouse/Rat Cathepsin C/DPPI Antibody

Detection of Mouse Cathepsin C/DPPI by Western Blot. Western blot shows lysates of mouse liver tissue and mouse spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cathepsin C/DPPI at approximately 20-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse and Rat Cathepsin C/DPPI by Western Blot. Western blot shows lysates of mouse lung tissue and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cathepsin C/DPPI at approximately 20-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cathepsin C/DPPI in Mouse Thymus. Cathepsin C/DPPI was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Cathepsin C/ DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific staining was localized to thymocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse Cathepsin C/DPPI by Simple Western^TM. Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for Cathepsin C/DPPI at approximately 35 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1034) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Detection of Mouse Cathepsin C/DPPI by Immunohistochemistry The expression pattern of Cat C mRNA and protein in the control brain. ISH (A and B) and IHC (C and D) staining were performed in control mice. The rectangles in A and C which included the CA2 area were enlarged into B and D, respectively. Cat C gene was predominantly expressed in the CA2 neurons of the hippocampus (B and D), interneurons (dotted arrow in C), choroid plexus (thick solid arrows in A and C), leptomeninges (open arrow in C) and vascular cells (thin solid arrow in C). Scale bar = 100 μm (A, C); 20 μM (B, D). n = 5 per condition. Cat C, cathepsin C; IHC, immunohistochemistry; ISH, in situ hybridization. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22607609), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Cathepsin C/DPPI by Immunohistochemistry The expression pattern of Cat C mRNA and protein in the control brain. ISH (A and B) and IHC (C and D) staining were performed in control mice. The rectangles in A and C which included the CA2 area were enlarged into B and D, respectively. Cat C gene was predominantly expressed in the CA2 neurons of the hippocampus (B and D), interneurons (dotted arrow in C), choroid plexus (thick solid arrows in A and C), leptomeninges (open arrow in C) and vascular cells (thin solid arrow in C). Scale bar = 100 μm (A, C); 20 μM (B, D). n = 5 per condition. Cat C, cathepsin C; IHC, immunohistochemistry; ISH, in situ hybridization. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22607609), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Cathepsin C/DPPI by Immunocytochemistry/ Immunofluorescence Myc induces cathepsin L expression in beta-cells of pancreatic Islets.(A) Immunohistochemical analyses for CTS B, C, L or S expression (all in red) in combination with staining for the pan-leukocyte marker CD45 (green) in pancreatic islet tumors from the MycERTAM;Bcl-xL animals. Pancreata were harvested from the MycERTAM;Bcl-xL mice treated for 7 d with TAM (Myc-On, 7 days) or control vehicle in place of TAM (Myc-OFF). The islet area is indicated by dotted line. The asterisks indicate the area of tumor represented in the insets. The panels are representatives of at least three animals assayed at each data point, all immunohistochemical analyses were done in duplicate; eight randomized fields per analysis were examined. Scale bars, 100μm. (B) Immunohistochemical analysis for cathepsin L expression in beta-cells of pancreatic islets from MycERTAM;Bcl-xL animals identified by insulin expression. Pancreata were collected from the animals described above. Scale bars represent 25μm. The panels are representatives of three animals assayed at each data point, all immunohistochemical analyses were done in duplicate; ten randomized fields per analysis were examined. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0120348), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Cathepsin C/DPPI by Immunocytochemistry/ Immunofluorescence The expression of Cat C was induced in the brain at six hours and twenty-four hours following LPS injection. IHC double staining showed that the Cat C expression was induced in cortical neurons at six hours after LPS injection (A to C). After LPS injection for twenty-four hours, the expression of Cat C mRNA and protein was induced further and distributed throughout the entire brain, such as the cortex (D and G), hippocampus (E and H) and SN (F and I). Double staining of Iba-1 (IHC) and Cat C (ISH) showed that Cat C expression was induced in microglial cells at twenty-four hours after LPS injection (J and K). Scale bar = 20 μm (A to C, J and K); 50 μm (D to I). n = 5 per condition. Cat C, cathepsin C; Iba-1, ionized calcium binding adaptor molecule-1; IHC, immunohistochemistry; ISH, in situ hybridization; LPS, lipopolysaccharide. SN, substantia nigra. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22607609), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Cathepsin C/DPPI by Immunohistochemistry The expression of Cat C was induced in the brain at six hours and twenty-four hours following LPS injection. IHC double staining showed that the Cat C expression was induced in cortical neurons at six hours after LPS injection (A to C). After LPS injection for twenty-four hours, the expression of Cat C mRNA and protein was induced further and distributed throughout the entire brain, such as the cortex (D and G), hippocampus (E and H) and SN (F and I). Double staining of Iba-1 (IHC) and Cat C (ISH) showed that Cat C expression was induced in microglial cells at twenty-four hours after LPS injection (J and K). Scale bar = 20 μm (A to C, J and K); 50 μm (D to I). n = 5 per condition. Cat C, cathepsin C; Iba-1, ionized calcium binding adaptor molecule-1; IHC, immunohistochemistry; ISH, in situ hybridization; LPS, lipopolysaccharide. SN, substantia nigra. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22607609), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Cathepsin C/DPPI by Immunohistochemistry The expression pattern of Cat C mRNA and protein in the control brain. ISH (A and B) and IHC (C and D) staining were performed in control mice. The rectangles in A and C which included the CA2 area were enlarged into B and D, respectively. Cat C gene was predominantly expressed in the CA2 neurons of the hippocampus (B and D), interneurons (dotted arrow in C), choroid plexus (thick solid arrows in A and C), leptomeninges (open arrow in C) and vascular cells (thin solid arrow in C). Scale bar = 100 μm (A, C); 20 μM (B, D). n = 5 per condition. Cat C, cathepsin C; IHC, immunohistochemistry; ISH, in situ hybridization. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22607609), licensed under a CC-BY license. Not internally tested by R&D Systems.