Home / Semaphorin 7A / Mouse Semaphorin 7A Antibody

Mouse Semaphorin 7A Antibody

Catalog #: AF1835
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1835
AF1835-SP
Detection of Human Semaphorin 7A by Simple WesternTM.
2 Images
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Semaphorin 7A Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Semaphorin 7A in direct ELISAs and Western blots. In these formats, less than 1% cross‑reactivity with recombinant mouse (rm) Semaphorin 3C, rmSemaphorin 3E, rmSemaphorin 3F, rmSemaphorin 6A, rmSemaphorin 6B, rmSemaphorin 6C, recombinant human (rh) Semaphorin 3A, rhSemaphorin 3B, and rhSemaphorin 6D is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 7A
Gln45-Ala646
Accession # Q9QUR8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
2.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Simple Western Detection of Human Semaphorin 7A antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Semaphorin 7A by Simple WesternTM. Simple Western lane view shows lysates of human brain (cortex) tissue, loaded at 0.2 mg/mL. A specific band was detected for Semaphorin 7A at approximately 109 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse Semaphorin 7A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1835) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Detection of Mouse Semaphorin 7A antibody by Western Blot. View Larger

Detection of Mouse Semaphorin 7A by Western Blot. Western blot shows lysates of mouse brain tissue, mouse testis tissue, and mouse ovary tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Semaphorin 7A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1835) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Semaphorin 7A at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 7A

Semaphorin 7A (Sema7A, designated CD108, previously Sema K1 or Sema L), is an ~80 kDa membrane-anchored glycoprotein that is a member of the Semaphorin family of axon guidance molecules (1 - 4). On human erythrocytes, it is the John Milton Hagen (JMH) blood group antigen (4). Sema7A is the only known Class 7 or glycophosphatidylinositol (GPI)-linked semaphorin; its expression is concentrated in the brain, spleen and thymus (1 - 5). Mouse Sema7A cDNA encodes a 44 amino acid (aa) signal sequence, a 602 aa extracellular domain (ECD) including Sema and C2-type Ig-like domains, and an 18 aa propeptide/GPI membrane anchor signal sequence. Mature mouse Sema7A shares 89%, 98%, 85%, 86% and 89% aa identity with corresponding human, rat, bovine, canine and equine Sema7A, respectively. The Sema7A sema domain contains an RGD integrin interaction motif (4). Although it binds plexin-C1 in vitro and may be coexpressed with it, many of its activities depend on interaction with beta 1 integrins such as alpha 1 beta 1 (6 - 10). Sema7A signaling through the two receptors may cause opposing effects (8). Sema7A is an immune semaphorin, with expression and activity on CD4+CD8+ thymocytes, activated T cells, macrophages and microglia (2, 9 - 12). T cell Sema7A interacts with monocytic cells, stimulating their chemotaxis, production of pro-inflammatory cytokines, and dendritic differentiation (5, 6). However, on the T cells themselves, Sema7A downregulates TCR signaling by promoting TCR internalization, modulating T cell responses (9). In lung macrophages, Sema7A is induced by TGF-beta and participates in TGF-beta -induced lung fibrosis (12). Sema7A is also expressed on pre-osteoblasts and osteoclasts, where it promotes migration and fusion, respectively; on keratinocytes, where it promotes melanocyte spreading and dendricity; and on some neurons, for example, promoting axon outgrowth in the developing olfactory tract (8, 10, 13).

References
  1. Yazdani, U. and J.R. Terman (2006) Genome Biol. 7:211.
  2. Kikutani, H. et al. (2007) Adv. Immunol. 93:121.
  3. Sato, Y. and (1998) Biochim. Biophys. Acta 1443:419.
  4. Yamada, A. et al. (1999) J. Immunol. 162:4094.
  5. Holmes, S. et al. (2002) Scand. J. Immunol. 56:270.
  6. Suzuki, K. et al. (2007) Nature 446:680.
  7. Pasterkamp, R.J. et al. (2007) BMC Dev. Biol. 7:98.
  8. Scott, G.A. et al. (2007) J. Invest. Dermatol. 128:151.
  9. Czopik, A.K. et al. (2006) Immunity 24:591.
  10. Pasterkamp, R.J. et al. (2003) Nature 424:398.
  11. Mine, T. et al. (2000) Tissue Antigens 55:429.
  12. Kang, H-R. et al. (2007) J. Exp. Med. 204:1083.
  13. Delorme, G. et al. (2005) Biol. Cell 97:589.
Entrez Gene IDs
8482 (Human); 20361 (Mouse)
Alternate Names
CD108 antigen; CD108; CD108MGC126696; CDw108; H-SEMA-K1; H-Sema-L; JMH blood group antigen; JMH; John-Milton-Hargen human blood group Ag; MGC126692; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin)7A (JMH blood group); sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin)7A; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A; Sema K1; Sema L; Sema7A; SEMAK1; SEMAL; SEMALJohn Milton Hagen blood group H-Sema K1; Semaphorin 7A; semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group); semaphorin K1; semaphorin L; semaphorin-7A; semaphorin-K1; semaphorin-L

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Mouse Semaphorin 7A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
Filter your results:

Filter by:

  1. Semaphorin 7A plays a critical role in TGF-beta1-induced pulmonary fibrosis
    Authors: Hye-Ryun Kang, Chun Geun Lee, Robert J. Homer, Jack A. Elias
    The Journal of Experimental Medicine
  2. Semaphorin7A patterns neural circuitry in the lateral line of the zebrafish
    Authors: Dasgupta, A;Reagor, CC;Paik, SP;Snow, LM;Jacobo, A;Hudspeth, AJ;
    eLife
    Species: Zebrafish
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. Sema7A/PlxnCl signaling triggers activity-dependent olfactory synapse formation
    Authors: N Inoue, H Nishizumi, H Naritsuka, H Kiyonari, H Sakano
    Nat Commun, 2018-05-09;9(1):1842.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Rewiring the taste system
    Authors: H Lee, LJ Macpherson, CA Parada, CS Zuker, NJP Ryba
    Nature, 2017-08-09;548(7667):330-333.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Semaphorin7A regulates neuroglial plasticity in the adult hypothalamic median eminence.
    Authors: Parkash J, Messina A, Langlet F, Cimino I, Loyens A, Mazur D, Gallet S, Balland E, Malone S, Pralong F, Cagnoni G, Schellino R, De Marchis S, Mazzone M, Pasterkamp R, Tamagnone L, Prevot V, Giacobini P
    Nat Commun, 2015-02-27;6(0):6385.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  6. Semaphorin 7A as a potential immune regulator and promising therapeutic target in rheumatoid arthritis
    Authors: Jianmin Xie, Hao Wang
    Arthritis Research & Therapy
  7. Egr2-dependent gene expression profiling and ChIP-Seq reveal novel biologic targets in T cell anergy
    Authors: Yan Zheng, Yuanyuan Zha, Robbert M. Spaapen, Rebecca Mathew, Kenneth Barr, Albert Bendelac et al.
    Molecular Immunology
  8. Semaphorin 7A Promotes Chemokine-Driven Dendritic Cell Migration
    Authors: Anoek van Rijn, Leonie Paulis, Joost te Riet, Angela Vasaturo, Inge Reinieren-Beeren, Alie van der Schaaf et al.
    The Journal of Immunology

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse Semaphorin 7A Antibody

There are currently no reviews for this product. Be the first to review Mouse Semaphorin 7A Antibody and earn rewards!

Have you used Mouse Semaphorin 7A Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review