Mouse TACI/TNFRSF13B Antibody

Catalog #: AF1041
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1041
AF1041-SP

Product Details

Citations (5)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TACI/TNFRSF13B Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TACI/TNFRSF13B in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human TACI is observed and less than 1% cross-reactivity with recombinant mouse BCMA/TNFRSF17 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TACI/TNFRSF13B
Phe5-Thr129
Accession # Q9ET35

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse TACI/TNFRSF13B Fc Chimera (Catalog # 1041-TC)

Flow Cytometry

2.5 µg/10⁶ cells

Mouse splenocytes

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.25-1 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of Recombinant Mouse BAFF/BLyS/TNFSF13B (Catalog # 2106-BF) to immobilized Recombinant Mouse TACI/TNFRSF13B Fc Chimera (Catalog # 1041-TC) coated at 1 µg/mL (100 µL/well). At 15 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TACI/TNFRSF13B

TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily and has been designated TNFRSF13B. TACI is a type III membrane protein with an extracellular N-terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine-rich domains. Within the TNFRSF, it shares the highest homology with B cell maturation factor (BCMA). TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Mouse TACI is a 249 amino acid (aa) protein consisting of a 129 aa extracellular domain, a 20 aa transmembrane domain, and a 100 aa intracellular domain. Mouse and human TACI share 54% aa identity.

References

Xia, X.Z. et al. (2000) J. Exp. Med. 192:137.
von Bulow, G.U. et al. (1997) Science 278:138.
Gross, J.A. et al. (2000) Nature 404:995.
Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
Yan, M. et al. (2000) Nature Immunol. 1:37.
Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.

Long Name

Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B

Entrez Gene IDs

23495 (Human); 57916 (Mouse); 102126451 (Cynomolgus Monkey)

Alternate Names

CD267 antigen; CD267; CVID; CVID2; FLJ39942; MGC133214; TACI; TACIMGC39952; TNFRSF13B; TNFRSF14B; Transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B; tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse TACI/TNFRSF13B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F
Leukemia, 2015-07-03;30(1):163-72.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
The transmembrane activator TACI triggers immunoglobulin class switching by activating B cells through the adaptor MyD88.
Authors: He B, Santamaria R, Xu W, Cols M, Chen K, Puga I, Shan M, Xiong H, Bussel JB, Chiu A, Puel A, Reichenbach J, Marodi L, Doffinger R, Vasconcelos J, Issekutz A, Krause J, Davies G, Li X, Grimbacher B, Plebani A, Meffre E, Picard C, Cunningham-Rundles C, Casanova JL, Cerutti A
Nat. Immunol., 2010-08-01;11(9):836-45.
Species: Human, Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Functional implication of BAFF synthesis and release in gangliosides-stimulated microglia.
Authors: Kim KS, Park JY, Jou I, Park SM
J. Leukoc. Biol., 2009-04-30;86(2):349-59.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Deficient TACI expression on B lymphocytes of newborn mice leads to defective Ig secretion in response to BAFF or APRIL.
Authors: Kanswal S, Katsenelson N, Selvapandiyan A, Bram RJ, Akkoyunlu M
J. Immunol., 2008-07-15;181(2):976-90.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
TNF superfamily member APRIL enhances midbrain dopaminergic axon growth and contributes to the nigrostriatal projection in vivo
Authors: Thomas G. McWilliams, Laura Howard, Sean Wyatt, Alun M. Davies
Experimental Neurology