Mouse Wnt-10b Antibody

Catalog #: AF2110 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2110
AF2110-SP

All Wnt-10b Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Wnt-10b Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Wnt-10b in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse (rm) Wnt-3a, rmWnt-4, rmWnt-5a, and rmWnt-8a is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Wnt-10b peptide
Leu42-Glu82, Arg263-Arg332
Accession # P48614

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Wnt‑10b

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-10b

Wnt-10b (also known as Wnt-12) is a 45 kDa glycoprotein involved in fetal limb bud development and adult hematopoiesis. Mouse Wnt-10b exists as two isoforms: a 361 amino acid (aa) long form and a 265 aa short form that lacks a 95 aa internal sequence. Wnt-10b is less than 60% aa identical to other Wnts, but mouse to human, Wnt-10b shows almost complete aa sequence identity at > 97%.

Long Name

Wingless-type MMTV Integration Site Family, Member 10B

Entrez Gene IDs

7480 (Human); 22410 (Mouse)

Alternate Names

protein Wnt-10b; Protein Wnt-12; SHFM6WNT-10B protein; wingless-type MMTV integration site family, member 10B; Wnt10b; Wnt-10b; WNT12; Wnt-12