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Mouse Wnt-8a Antibody

Catalog #: AF2248
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2248
AF2248-SP
Product Details
Citations (3)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Wnt-8a Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Wnt-8a in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Wnt-1, rmWnt-3A, rmWnt-4, rmWnt-5A, and rmWnt-10B is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Wnt‑8a peptide
Trp23-Glu53, Leu196-Arg273
Accession # Q64527
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Wnt‑8a
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of mouse embryo (E13.5-15.5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-8a

Wnt-8A is a secreted glycoprotein that binds to receptors of the Frizzled family. It is expressed during early optic lens development and cardiac myogenesis.

Long Name
Wingless-type MMTV Integration Site Family, Member 8a
Entrez Gene IDs
7478 (Human); 20890 (Mouse)
Alternate Names
protein Wnt-8a; Protein Wnt-8d; wingless-type MMTV integration site family, member 8A; Wnt8a; Wnt-8a; WNT8DWNT8d

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Citations for Mouse Wnt-8a Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Labeling human embryonic stem cell-derived cardiomyocytes with indocyanine green for noninvasive tracking with optical imaging: an FDA-compatible alternative to firefly luciferase
    Authors: Sophie E. Boddington, Tobias D. Henning, Priyanka Jha, Christopher R. Schlieve, Lydia Mandrussow, David DeNardo et al.
    Cell Transplant
  2. Arsenic inhibits stem cell differentiation by altering the interplay between the Wnt3a and Notch signaling pathways
    Authors: Lisa J. Bain, Jui-Tung Liu, Ryan E. League
    Toxicology Reports
  3. Canonical Wnt signaling is a positive regulator of mammalian cardiac progenitors.
    Authors: Kwon C, Arnold J, Hsiao EC, Taketo MM, Conklin BR, Srivastava D
    Proc. Natl. Acad. Sci. U.S.A., 2007-06-18;104(26):10894-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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