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Rat beta-NGF Antibody

Catalog #: AF-556-NA
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-556-NA
AF-556-SP
beta -NGF in Rat Brain.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat beta-NGF Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat  beta -NGF in ELISAs and Western blots. In ELISAs, less than 0.1% cross-reactivity with recombinant human (rh)  beta ‑NGF, rhBDNF, recombinant rat (rr) GDNF, rhCNTF, rrGDNF R alpha, rrCNTF, rhGDNF, rhNT-3 and rhNT‑4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat beta -NGF (R&D Systems, Catalog # 556-NG)
Ser122-Gly241
Accession # P25427
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat beta -NGF (Catalog # 556-NG)
Immunohistochemistry
5-15 µg/mL
See below

Rat beta -NGF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Rat beta-NGF Biotinylated Antibody (Catalog # BAF556)

Standard: Recombinant Rat beta-NGF Protein (Catalog # 556-NG)

Neutralization
Measured by its ability to neutralize beta ‑NGF-induced proliferation in the TF‑1 human erythroleukemic cell line [Kitamura, T. et al. (1989) J. Cell Physiol. 140:323]. The Neutralization Dose (ND50) is typically 0.02-0.1 µg/mL in the presence of 3 ng/mL Recombinant Rat beta ‑NGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry beta -NGF antibody in Rat Brain by Immunohistochemistry (IHC-Fr). View Larger

beta -NGF in Rat Brain. beta -NGF was detected in perfusion fixed frozen sections of rat brain using Rat beta -NGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-556-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Neutralization Cell Proliferation Induced by beta ‑NGF and Neutralization by Rat beta ‑NGF Antibody. View Larger

Cell Proliferation Induced by beta ‑NGF and Neutralization by Rat beta ‑NGF Antibody. Recombinant Rat beta -NGF (Catalog # 556-NG) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat beta -NGF (3 ng/mL) is neutralized (green line) by increasing concentrations of Rat beta -NGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-556-NA). The ND50 is typically 0.02-0.1 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-NGF

NGF was initially isolated in the mouse submandibular gland over three decades ago as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant rat beta -NGF is a homodimer of two 120-amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotrophic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Long Name
beta Nerve Growth Factor
Entrez Gene IDs
4803 (Human); 18049 (Mouse); 310738 (Rat); 403402 (Canine); 103350089 (Rabbit)
Alternate Names
Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428

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Citations for Rat beta-NGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Systemic Depletion of Nerve Growth Factor Inhibits Disease Progression in a Genetically Engineered Model of Pancreatic Ductal Adenocarcinoma
    Authors: Jami L. Saloman, Aatur D. Singhi, Douglas J. Hartman, Daniel P. Normolle, Kathryn M. Albers, Brian M. Davis
    Pancreas
  2. Effect of nutritional supplement on bone marrow-derived mesenchymal stem cells from aplastic anaemia
    Authors: S Luo, Y Chen, L Zhao, X Qi, X Miao, H Zhou, L Jia
    Br. J. Nutr., 2018-04-01;119(7):748-758.
  3. Wogonin, a Bioactive Ingredient from Huangqi Guizhi Formula, Alleviates Discogenic Low Back Pain via Suppressing the Overexpressed NGF in Intervertebral Discs
    Authors: J Li, W Duan, S Chai, Y Luo, Y Ma, N Yang, M Liu, W He
    Mediators of Inflammation, 2023-02-20;2023(0):4436587.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  4. Intranasal Administration of Forskolin and Noopept Reverses Parkinsonian Pathology in PINK1 Knockout Rats
    Authors: RK Dagda, RY Dagda, E Vazquez-Ma, B Martinez, A Gallahue
    International Journal of Molecular Sciences, 2022-12-30;24(1):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Knockdown of astrocytic Grin2a aggravates beta-amyloid-induced memory and cognitive deficits through regulating nerve growth factor
    Authors: Z Du, Y Song, X Chen, W Zhang, G Zhang, H Li, L Chang, Y Wu
    Aging Cell, 2021-07-22;0(0):e13437.
    Species: Rat
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  6. Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain.
    J Neuroinflammation, 2012-07-23;9(0):181.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Bradykinin and nerve growth factor play pivotal roles in muscular mechanical hyperalgesia after exercise (delayed-onset muscle soreness).
    Authors: Murase S, Terazawa E, Queme F, Ota H, Matsuda T, Hirate K, Kozaki Y, Katanosaka K, Taguchi T, Urai H, Mizumura K
    J. Neurosci., 2010-03-10;30(10):3752-61.
    Species: Rat
    Sample Types: In Vivo
    Applications: Neutralization
  8. Electrical stimulation of sympathetic neurons induces autocrine/paracrine effects of NGF mediated by TrkA.
    Authors: Saygili E, Schauerte P, Kuppers F, Heck L, Weis J, Weber C, Schwinger RH, Hoffmann R, Schroder JW, Marx N, Rana OR
    J. Mol. Cell. Cardiol., 2010-02-02;49(1):79-87.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  9. The influence of inhibiting renal neural regeneration on the efficacy of renal denervation to chronic heart failure
    Authors: Pingan Chen, Zhiqin Guo, Yufeng Chen, Lushan Chen, Shaonan Li, Yanlin Xian et al.
    ESC Heart Failure
  10. Enteric glia mediate neuronal outgrowth through release of neurotrophic factors
    Authors: Christopher R. Hansebout, Caixin Su, Kiran Reddy, Donald Zhang, Cai Jiang, Michel P. Rathbone et al.
    Neural Regen Res

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