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Cotton Rat IL-1 beta /IL-1F2 Antibody

Catalog #: AF1009
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1009
AF1009-SP
Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Cotton Rat IL‑1 beta / IL‑1F2 Antibody.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Cotton Rat IL-1 beta /IL-1F2 Antibody Summary

Species Reactivity
Cotton Rat
Specificity
Detects cotton rat IL‑1 beta /IL‑1F2 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human IL-1 beta and recombinant mouse IL-1 beta is observed, and 10% cross‑reactivity with recombinant rat IL-1 beta and recombinant porcine IL-1 beta is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant cotton rat IL‑1 beta
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Cotton Rat IL‑1 beta /IL‑1F2 (Catalog # 1009-CL)
Neutralization
Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.4-1.2 µg/mL in the presence of 2 ng/mL Recombinant Cotton Rat IL‑1 beta /IL‑1F2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Cotton Rat IL‑1 beta / IL‑1F2 Antibody. View Larger

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Cotton Rat IL‑1 beta / IL‑1F2 Antibody. Recombinant Cotton Rat IL-1 beta /IL-1F2 (1009-CL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-1 beta /IL-1F2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL-1 beta / IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1009). The ND50 is typically 0.4-1.2 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in cotton rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1‑4). The cotton rat IL-1 beta cDNA encodes a 267 aa precursor. A 115 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature cotton rat IL-1 beta shares 90% aa sequence identity with mouse and rat and 65%‑78% with canine, equine, feline, human, porcine, and rhesus IL-1 beta.

References
  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. Accession # AAL18817.
  6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

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Citations for Cotton Rat IL-1 beta /IL-1F2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Unveiling Integrated Functional Pathways Leading to Enhanced Respiratory Disease Associated With Inactivated Respiratory Syncytial Viral Vaccine.
    Authors: Russell Marsha S, Creskey Marybeth, Muralidharan Abenaya et al.
    Frontiers in Immunology
  2. Epidermal Growth Factor Receptor-Dependent Mutual Amplification between Netrin-1 and the Hepatitis C Virus.
    Authors: Plissonnier Marie-Laure, Lahlali Thomas, Michelet Maud et al.
    PLoS Biol

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