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Equine IL-6 Antibody

Catalog #: AF1886
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1886
AF1886-SP
Cell Proliferation Induced by IL‑6 and Neutralization by Equine IL‑6 Antibody.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Equine IL-6 Antibody Summary

Species Reactivity
Equine
Specificity
Detects equine IL‑6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human IL-6, recombinant porcine IL-6 and recombinant canine IL-6 is observed and approximately 20% cross-reactivity with recombinant mouse IL-6 and recombinant rat IL-6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant equine IL‑6
Phe26-Met208
Accession # Q95181
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Equine IL‑6 (Catalog # 1886-EL)
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R. P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 4 ng/mL Recombinant Equine IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑6 and Neutralization by Equine IL‑6 Antibody. View Larger

Cell Proliferation Induced by IL‑6 and Neutralization by Equine IL‑6 Antibody. Recombinant Equine IL-6 (Catalog # 1886-EL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Equine IL-6 (4 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1886). The ND50 is typically 0.5-1.5 µg/mL.

Immunocytochemistry IL-6 antibody in Equine PBMCs by Immunocytochemistry (ICC). View Larger

IL‑6 in Equine PBMCs. IL-6 was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Equine IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1886) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6

Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22 kDa‑28 kDa phosphorylated and variably glycosylated molecule (1‑4). Mature equine IL-6 is 181 amino acids (aa) in length and shares 61%, 42%, and 43% aa sequence identity with human, mouse, and rat IL-6 (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL‑6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8).

References
  1. Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.
  2. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.
  3. Jones, S.A. (2005) J. Immunol. 175:3468.
  4. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.
  5. Swiderski, S.E. et al. Vet. Immunol. Immunopathol. 77:213.
  6. Murakami, M. et al. (1993) Science 260:1808.
  7. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
  8. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
Long Name
Interleukin 6
Entrez Gene IDs
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names
B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

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Citations for Equine IL-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Serum Health Biomarkers in African and Asian Elephants: Value Ranges and Clinical Values Indicative of the Immune Response
    Authors: Katie L. Edwards, Michele A. Miller, Jessica Siegal-Willott, Janine L. Brown
    Animals (Basel)
  2. Tendon extracellular matrix damage, degradation and inflammation in response to in vitro overload exercise
    Authors: Ewa M. Spiesz, Chavaunne T. Thorpe, Saira Chaudhry, Graham P. Riley, Helen L. Birch, Peter D. Clegg et al.
    Journal of Orthopaedic Research
  3. Equine mesenchymal stem cells inhibit T cell proliferation through different mechanisms depending on tissue source.
    Authors: Carrade Holt D, Wood J, Granick J, Walker N, Clark K, Borjesson D
    Stem Cells Dev, 2014-03-04;23(11):1258-65.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Serum interleukin-6 (IL-6) and IL-10 concentrations in normal and septic neonatal foals.
    Authors: Burton AB, Wagner B, Erb HN, Ainsworth DM
    Vet. Immunol. Immunopathol., 2009-05-18;132(2):122-8.
    Species: Equine
    Sample Types: Colostrum, Serum
    Applications: ELISA Capture
  5. Radiation-induced loss of cell surface CD47 enhances immune-mediated clearance of human papillomavirus-positive cancer.
    Authors: Vermeer D, Spanos W, Vermeer P, Bruns A, Lee K, Lee J
    Int J Cancer, 2013-02-12;133(1):120-9.

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