Feline IL-8/CXCL8 Antibody

Catalog #: AF2277 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2277
AF2277-SP

Chemotaxis Induced by IL-8/CXCL8 and Neutral-ization by Feline IL-8/CXCL8 Antibody.

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Feline IL-8/CXCL8 Antibody Summary

Species Reactivity

Feline

Specificity

Detects feline IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross‑reactivity with recombinant canine IL-8/CXCL8 is observed and 15% cross-reactivity with recombinant porcine IL-8/CXCL8 and recombinant human IL-8/CXCL8 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant feline IL-8/CXCL8
Ala23-Ala101
Accession # Q9XSX5.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Feline IL-8/CXCL8 (Catalog # 2277-FL)

Immunocytochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize IL-8/CXCL8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.1-0.4 µg/mL in the presence of 20 ng/mL Recombinant Feline IL-8/CXCL8.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by IL-8/CXCL8 and Neutral-ization by Feline IL-8/CXCL8 Antibody. Recombinant Feline IL-8/CXCL8 (Catalog # 2277-FL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Feline IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2277). The ND₅₀ is typically 0.1-0.4 µg/mL.

Immunocytochemistry

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IL-8/CXCL8 in Feline PBMCs. IL-8/CXCL8 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2277) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-8/CXCL8

Interleukin 8 (IL-8), also named CXCL8, monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil-activating protein 1 (NAP-1), neutrophil-activating factor (NAF) and granulocyte chemotactic peptide (GCP), belongs to the Glu-Leu-Arg motif containing (ELR+) CXC chemokine family and has been designated CXCL8. IL-8 is a potent neutrophil chemoattractant that recruits neutrophils to sites of inflammation. IL-8 also activates neutrophil functions and promotes angiogenesis. The biological activites of IL-8 is mediated by two types of G protein-coupled chemokine receptors, CXCR1 and CXCR2 (1, 2). In normal tissues, IL-8 expression and secretion is barely detectable. Upon stimulation by a wide range of pro-inflammatory signals including exposure to IL-1, TNF, bacterial or viral products, IL-8 production is rapidly induced in many different cell types (3, 4). Feline IL-8 encodes a 101 amino acid (aa) precursor protein with a putative 22 aa signal peptide. It shares 61% and 76% aa sequence identity with human and canine IL-8, respectively.

References

Van Damme, J. et al. (1998) The Cytokine Handbook, A.W. Thomson, ed., Academic Press, NY p. 271.
Heidemann, J. et al. (2003) J. Biol. Chem. 178:8508.
Yang, M.P. et al. (2002) Vet. Immunol. Immunopathol. 86:43.
Parhar, K. et al. (2003) Immunology 108:502.

Long Name

Interleukin 8

Entrez Gene IDs

3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)

Alternate Names

3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1