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Human ABCG2 Antibody

Catalog #: MAB995
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB995-SP
MAB995-500
MAB995-100
ABCG2 in JAR Human Cell Line.
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Product Details
Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ABCG2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ABCG2 in flow cytometry and immunocytochemistry.
Source
Monoclonal Mouse IgG2B Clone # 5D3
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
3T3 cells transduced with human ABCG2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human ABCG2 transfected CHO-S cells
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry ABCG2 antibody in JAR Human Cell Line by Immunocytochemistry (ICC). View Larger

ABCG2 in JAR Human Cell Line. ATP-Binding Cassette Transporter G2 (ABCG2) was detected in immersion fixed JAR human choriocarcinoma cell line using Mouse Anti-Human ABCG2 Monoclonal Antibody (Catalog # MAB995) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; NL009) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry ABCG2 antibody in Human ABCG2 transfected CHO Chinese Hamster Cell Line by Immunocytochemistry (ICC). View Larger

ABCG2 in Human ABCG2 transfected CHO Chinese Hamster Cell Line. ABCG2 was detected in immersion fixed Human ABCG2 transfected CHO Chinese hamster ovary cell line using Mouse Anti-Human ABCG2 Monoclonal Antibody (Catalog # MAB995) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry View Larger

ABCG2 in A549 Human Cell Line. ABCG2 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human ABCG2 Monoclonal Antibody (Catalog # MAB995) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Flow Cytometry View Larger

Detection of ABCG2 in RPMI 8226 cells by Flow Cytometry RPMI 8226 cells were stained with Mouse Anti-Human ABCG2 Monoclonal Antibody (Catalog # MAB995, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ABCG2

Hematopoietic stem cells are known to express a membrane transporter molecule, known as P-glycoprotein (Pgp), that is encoded by the multidrug resistance gene 1 (MDR1) (1, 2). Expression of Pgp appears to confer a proliferative advantage to stem cells through its anti-apoptotic effects (3, 4). An additional transporter molecule known as ABCG2 (ATP-binding cassette gene 2) or Bcrp1 (Breast cancer resistance protein 1), first identified in a breast cancer cell line (5), is expressed on stem cells (6). ABCG2 belongs to a family of molecules that span the cell membrane six times and can exist as either homo or hetero dimers linked by a short intracellular flexible linker region that plays an important role in the efflux of a wide range of substrates (7, 8). Although these transporter molecules have initially been thought to play a role in drug resistance, they have been found to have utility in better characterizing primitive stem cells. For example, the “side-population” of hematopoietic stem cells, characterized by their inability to retain high levels of the intracellular staining dyes Hoechst 33342 and Rhodamine 123, has been found to express high levels of ABCG2. Of interest is the observation that ABCG2 function has been linked to the efflux of the Hoechst dye (6). Furthermore, there is now evidence that this monoclonal can be used as a cell surface marker to identify hematopoietic stem cells within the bone marrow fraction of lineage negative cells (6). The expression of ABCG2 appears greatest on CD34- cells and is downregulated with the acquisition of CD34 on the cell surface (6).

References
  1. Chaudhary, P.M. and I.B. Roninson (1991) Cell 66:85.
  2. Sorrentino, B.P. et al. (1995) Blood 86:491.
  3. Pallis, M. and N. Russell (2000) Blood 95:2897.
  4. Johnstone, R.W. et al. (1999) Blood 93:1075.
  5. Doyle, L.A. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15665.
  6. Zhou, S. et al. (2001) Nat. Medicine 7:1028.
  7. Hrycyna, C.A. et al. (1998) Biochem. 37:13660.
  8. Bunting, K.D. (2002) Stem Cells 20:11.
Long Name
ATP-binding Cassette Transporter G2
Entrez Gene IDs
9429 (Human)
Alternate Names
ABC transporter; ABC15; ABCG2; ABCP; ABCPMGC102821; ATP-binding cassette sub-family G member 2; ATP-binding cassette transporter G2; ATP-binding cassette, sub-family G (WHITE), member 2; BCRP; BCRP1; BCRPMRX; BMDP; Breast cancer resistance protein; CD338 antigen; CD338; CDw338; EST157481; mitoxantrone resistance protein; Mitoxantrone resistance-associated protein; MXR; MXRMXR1; placenta specific MDR protein; Placenta-specific ATP-binding cassette transporter

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Citations for Human ABCG2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Microbial metabolite restricts 5-fluorouracil-resistant colonic tumor progression by sensitizing drug transporters via regulation of FOXO3-FOXM1 axis
    Authors: S Ghosh, R Singh, ZM Vanwinkle, H Guo, PK Vemula, A Goel, B Haribabu, VR Jala
    Theranostics, 2022-07-18;12(12):5574-5595.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Transcriptional Heterogeneity in Naive and Primed Human Pluripotent Stem Cells at Single-Cell Resolution
    Authors: T Messmer, F von Meyenn, A Savino, F Santos, H Mohammed, ATL Lun, JC Marioni, W Reik
    Cell Rep, 2019-01-22;26(4):815-824.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Effect of NOS1 regulating ABCG2 expression on proliferation and apoptosis of cervical cancer cells
    Authors: M Ding, H Zhang, L Liu, R Liang
    Oncol Lett, 2018-11-30;17(2):1531-1536.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. In�Vitro Modeling of Blood-Brain Barrier with Human iPSC-Derived Endothelial Cells, Pericytes, Neurons, and Astrocytes via Notch Signaling
    Authors: K Yamamizu, M Iwasaki, H Takakubo, T Sakamoto, T Ikuno, M Miyoshi, T Kondo, Y Nakao, M Nakagawa, H Inoue, JK Yamashita
    Stem Cell Reports, 2017-02-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. The Metastatic Potential and Chemoresistance of Human Pancreatic Cancer Stem Cells.
    Authors: Bhagwandin V, Bishop J, Wright W, Shay J
    PLoS ONE, 2016-02-09;11(2):e0148807.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Effect of culture medium on propagation and phenotype of corneal stroma–derived stem cells
    Authors: Laura E. Sidney, Matthew J. Branch, Harminder S. Dua, Andrew Hopkinson
    Cytotherapy
  7. Expression of Potential Cancer Stem Cell Marker ABCG2 is Associated with Malignant Behaviors of Hepatocellular Carcinoma
    Authors: Guang Zhang, Zhongxia Wang, Weihuan Luo, Hongbo Jiao, Junhua Wu, Chunping Jiang
    Gastroenterology Research and Practice
  8. Mitochondrial localization of ABC transporter ABCG2 and its function in 5-aminolevulinic acid-mediated protoporphyrin IX accumulation.
    Authors: Kobuchi, Hirotsug, Moriya, Koko, Ogino, Tetsuya, Fujita, Hirofumi, Inoue, Keiji, Shuin, Taro, Yasuda, Tatsuji, Utsumi, Kozo, Utsumi, Toshihik
    PLoS ONE, 2012-11-26;7(11):e50082.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  9. Human umbilical cord blood-derived non-hematopoietic stem cells suppress lymphocyte proliferation and CD4, CD8 expression.
    Authors: Ji F, Wang Y, Sun H, Du J, Zhao H, Wang D, Xu Q, Duan D, Yang H
    J. Neuroimmunol., 2008-06-04;197(2):99-109.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. CD molecules 2006--human cell differentiation molecules.
    Authors: Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
    J. Immunol. Methods, 2006-12-04;319(1):1-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Isolation of multipotent neural crest-derived stem cells from the adult mouse cornea.
    Authors: Shimmura S, Nagoshi N, Matsuzaki Y, Okano H, Tsubota K
    Stem Cells, 2006-08-03;24(12):2714-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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