Home / Cathepsin D / Human Cathepsin D Antibody

Human Cathepsin D Antibody

Catalog #: AF1014
31 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1014
AF1014-SP
Detection of Human Cathepsin D by Western Blot.
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Product Details
Citations (31)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cathepsin D Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cathepsin D in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) Cathepsin D is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin D
Leu21-Leu412
Accession # P07339
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Cathepsin D (Catalog # 1014-AS), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Cathepsin D antibody by Western Blot. View Larger

Detection of Human Cathepsin D by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1014) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Procathepsin D at approximately 45 kDa and Cathepsin D heavy chain 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human Cathepsin D antibody by Western Blot. View Larger

Detection of Human Cathepsin D by Western Blot. Western blot shows lysates of PANC-1 human pancreatic carcinoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1014) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Procathepsin D at approximately 45 kDa and Cathepsin D heavy chain 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Cathepsin D antibody in Human Lung Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Cathepsin D in Human Lung Cancer Tissue. Cathepsin D was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Goat Anti-Human Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1014) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human Cathepsin D antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Cathepsin D by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. Specific bands were detected for Procathepsin D at approximately 55 kDa and Cathepsin D heavy chain at approximately 37 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1014) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin D

Cathepsin D is a lysosomal aspartic protease of the pepsin family (1). Human cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑18), a propeptide (residues 19-64), and a mature chain (residues 65‑412) (2‑4). The mature chain can be processed further to the light (residues 65‑161) and heavy (residues 169‑412) chains. It is expressed in most cells and overexpressed in breast cancer cells (5). It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis (6). Cathepsin D secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogeneous inhibitor of angiogenesis (6).

References
  1. Conner et al. in Handbook of Proteolytic Enzymes Barrett (1998) Academic Press, San Diego, p. 828.
  2. Faust, et al. (1985) Proc. Natl. Acad. Sci. USA 82:4910.
  3. Westley and May (1987) Nucl. Acid Res. 15:3773.
  4. Redecker, et al. (1991) DNA Cell Biol. 10:423.
  5. Rochefort, et al. (2000) Clin. Chim. Acta. 291:157.
  6. Tsukuba, et al. (2000) Mol. Cells 10:601.
Entrez Gene IDs
1509 (Human); 13033 (Mouse)
Alternate Names
cathepsin D (lysosomal aspartyl protease); Cathepsin D; CPSD; CTSD; EC 3.4.23; EC 3.4.23.5; lysosomal aspartyl peptidase; lysosomal aspartyl protease; MGC2311; neuronal 10

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Citations for Human Cathepsin D Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. Discovery of Small Molecules That Induce Lysosomal Cell Death in Cancer Cell Lines Using an Image-Based Screening Platform
    Authors: David A Egan
    Assay Drug Dev Technol, 2016-09-22;14(8):489-510.
  2. Schwann Cell Stimulation of Pancreatic Cancer Cells: A Proteomic Analysis
    Authors: Aysha Ferdoushi, Xiang Li, Nathan Griffin, Sam Faulkner, M. Fairuz B. Jamaluddin, Fangfang Gao et al.
    Frontiers in Oncology
  3. Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe
    Authors: Shunsuke Ishii, Akira Matsuura, Eisuke Itakura
    Scientific Reports
  4. Optogenetic Tools for Manipulating Protein Subcellular Localization and Intracellular Signaling at Organelle Contact Sites
    Authors: Lorena Benedetti
    Current Protocols
  5. Quantitative proteomic analysis of gastric cancer tissue reveals novel proteins in platelet-derived growth factor B signaling pathway
    Authors: Fang Liu, Yuan Zhang, Tingting Men, Xingyue Jiang, Chunhua Yang, He Li et al.
    Oncotarget
  6. Negative Modulation of Macroautophagy by Stabilized HERPUD1 is Counteracted by an Increased ER-Lysosomal Network With Impact in Drug-Induced Stress Cell Survival
    Authors: Gabriela Vargas, Omar Cortés, Eloisa Arias-Muñoz, Sergio Hernández, Cristobal Cerda-Troncoso, Laura Hernández et al.
    Frontiers in Cell and Developmental Biology
  7. Inhibition of endoplasmic reticulum associated degradation reduces endoplasmic reticulum stress and alters lysosomal morphology and distribution
    Authors: Elfrink HL, Zwart R, Baas F, Scheper W.
    Mol Cells
  8. Myocardial cathepsin D is downregulated in sudden cardiac death
    Authors: Yu Kakimoto, Ayumi Sasaki, Maki Niioka, Noboru Kawabe, Motoki Osawa
    PLOS ONE
  9. Progranulin Stimulates the In Vitro Maturation of Pro-Cathepsin D at Acidic pH
    Authors: Victoria J. Butler, Wilian A. Cortopassi, Andrea R. Argouarch, Sam L. Ivry, Charles S. Craik, Matthew P. Jacobson et al.
    Journal of Molecular Biology
  10. Impaired Lysosome Reformation in Chloroquine-Treated Retinal Pigment Epithelial Cells
    Authors: Cardoso, MH;Hall, MJ;Burgoyne, T;Fale, P;Storm, T;Escrevente, C;Antas, P;Seabra, MC;Futter, CE;
    Investigative ophthalmology & visual science
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Wild-type and pathogenic forms of ubiquilin 2 differentially modulate components of the autophagy-lysosome pathways
    Authors: Idera, A;Sharkey, LM;Kurauchi, Y;Kadoyama, K;Paulson, HL;Katsuki, H;Seki, T;
    Journal of pharmacological sciences
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. A Practical and Analytical Comparative Study of Gel-Based Top-Down and Gel-Free Bottom-Up Proteomics Including Unbiased Proteoform Detection
    Authors: Ercan H, Resch U, Hsu F et al.
    Cells
  13. An infection-induced oxidation site regulates legumain processing and tumor growth
    Authors: Y Kovalyova, DW Bak, EM Gordon, C Fung, JHB Shuman, TL Cover, MR Amieva, E Weerapana, SK Hatzios
    Nature Chemical Biology, 2022-03-24;0(0):.
    Species: Gerbil
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Palmitic and Stearic Acids Inhibit Chaperone-Mediated Autophagy (CMA) in POMC-like Neurons In Vitro
    Authors: R Espinosa, K Gutiérrez, J Rios, F Ormeño, L Yantén, P Galaz-Davi, CA Ramírez-Sa, V Parra, A Albornoz, IE Alfaro, PV Burgos, E Morselli, A Criollo, M Budini
    Cells, 2022-03-08;11(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  15. Individually cultured bovine embryos produce extracellular vesicles that have the potential to be used as non-invasive embryo quality markers
    Authors: K Dissanayak, M Nõmm, F Lättekivi, Y Ressaissi, K Godakumara, A Lavrits, G Midekessa, J Viil, R Bæk, MM Jørgensen, S Bhattachar, A Andronowsk, A Salumets, Ü Jaakma, A Fazeli
    Theriogenology, 2020-04-04;149(0):104-116.
    Species: Mouse
    Sample Types:
    Applications: Microarray
  16. Premature termination codon readthrough upregulates progranulin expression and improves lysosomal function in preclinical models of GRN deficiency
    Authors: J Frew, A Baradaran-, AD Balgi, X Wu, TD Yan, S Arns, FS Shidmoossa, J Tan, JB Jaquith, KR Jansen-Wes, FC Lynn, FB Gao, L Petrucelli, HH Feldman, IR Mackenzie, M Roberge, HB Nygaard
    Mol Neurodegener, 2020-03-16;15(1):21.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Identification of PIKfyve kinase as a target in multiple myeloma
    Authors: C Bonolo De, YX Zhu, N Sepetov, S Romanov, LA Bruins, CX Shi, CK Stein, JL Petit, AN Polito, ME Sharik, EW Meermeier, GJ Ahmann, ID Lopez Arme, J Kruse, PL Bergsagel, M Chesi, N Meurice, E Braggio, AK Stewart
    Haematologica, 2019-10-03;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Visualizing the cellular route of entry of a cystine-knot peptide with Xfect transfection reagent by electron microscopy
    Authors: X Gao, A De Mazière, DB Iaea, CP Arthur, J Klumperman, C Ciferri, RN Hannoush
    Sci Rep, 2019-05-06;9(1):6907.
    Species: Human
    Sample Types: Whole Cells
    Applications: Electron Microscopy
  19. HSP90 inhibition targets autophagy and induces a CASP9-dependent resistance mechanism in NSCLC
    Authors: J Han, LA Goldstein, W Hou, S Chatterjee, TF Burns, H Rabinowich
    Autophagy, 2018-03-21;0(0):1-14.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  20. Amphiphilic star PEG-Camptothecin conjugates for intracellular targeting
    J Control Release, 2016-09-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  21. The Apaf-1-binding protein Aven is cleaved by Cathepsin D to unleash its anti-apoptotic potential.
    Cell Death Differ., 2012-03-02;19(9):1435-45.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. IFN-gamma regulation of vacuolar pH, cathepsin D processing and autophagy in mammary epithelial cells.
    Authors: Khalkhali-Ellis Z, Abbott DE, Bailey CM, Goossens W, Margaryan NV, Gluck SL, Reuveni M, Hendrix MJ
    J. Cell. Biochem., 2008-09-01;105(1):208-18.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  23. Clathrin is a key regulator of basolateral polarity.
    Authors: Deborde S, Perret E, Gravotta D, Deora A, Salvarezza S, Schreiner R, Rodriguez-Boulan E
    Nature, 2008-04-10;452(7188):719-23.
    Species: Canine
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  24. Efficient progranulin exit from the ER requires its interaction with prosaposin, a Surf4 cargo
    Authors: Swathi Devireddy, Shawn M. Ferguson
    Journal of Cell Biology
  25. A Practical and Analytical Comparative Study of Gel-Based Top-Down and Gel-Free Bottom-Up Proteomics Including Unbiased Proteoform Detection
    Authors: Ercan H, Resch U, Hsu F et al.
    Cells
  26. Retromer has a selective function in cargo sorting via endosome transport carriers
    Authors: Cui Y, Carosi JM, Yang Z et al.
    J. Cell Biol.
  27. Platelets, endothelial cells and leukocytes contribute to the exercise-triggered release of extracellular vesicles into the circulation
    Authors: Alexandra Brahmer, Elmo Neuberger, Leona Esch-Heisser, Nils Haller, Malene Moeller Jorgensen, Rikke Baek et al.
    Journal of Extracellular Vesicles
  28. High Resolution Proteomic Analysis of the Cervical Cancer Cell Lines Secretome Documents Deregulation of Multiple Proteases
    Authors: Kalliopi I Pappa, Georgia Kontostathi, Manousos Makridakis, Vasiliki Lygirou, Jerome Zoidakis, George Daskalakis et al.
    Cancer Genomics & Proteomics
  29. Chemoproteomic profiling reveals that cathepsin D off-target activity drives ocular toxicity of beta -secretase inhibitors
    Authors: Andrea M. Zuhl, Charles E. Nolan, Michael A. Brodney, Sherry Niessen, Kevin Atchison, Christopher Houle et al.
    Nature Communications
  30. Quantitative correlative microscopy reveals the ultrastructural distribution of endogenous endosomal proteins
    Authors: Jan van der Beek, Cecilia de Heus, Nalan Liv, Judith Klumperman
    Journal of Cell Biology
  31. Reevaluating cathepsin D as a biomarker for breast cancer serum activity levels versus histopathology
    Authors: Daniel E. Abbott, Naira V. Margaryan, Jacqueline S. Jeruss, Seema Khan, Virginia Kaklamani, David J. Winchester et al.
    Cancer Biol Ther

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