Human CCL5/RANTES Antibody

Name: Human CCL5/RANTES Antibody
Brand: R&D Systems
SKU: AB-278-NA
Rating: 5 (1 reviews)

Catalog #: AB-278-NA
2 Citations 1 Review Datasheet / COA / SDS

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AB-278-NA

Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody.

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All CCL5/RANTES Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL5/RANTES Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCL5/RANTES in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse C10 is observed.

Source

Polyclonal Goat IgG

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human CCL5/RANTES
Ser24-Ser91
Accession # P13501

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CCL5/RANTES (Catalog # 278-RN)

Neutralization

Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in human monocytes. The Neutralization Dose (ND₅₀) is typically 10-30 µg/mL in the presence of 0.2 µg/mL Recombinant Human CCL5/RANTES.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody. Recombinant Human CCL5/ RANTES (Catalog # 278-RN) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through to the membrane was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human CCL5/ RANTES (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL5/RANTES Polyclonal Antibody (Catalog # AB-278-NA). The ND₅₀ is typically 10-30 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL5/RANTES

CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta ‑chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1‑3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature human CCL5 shares 75%‑84% aa seqeuence identity with canine, cotton rat, feline, mouse, and rat CCL5 (5). CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (6, 7). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (8). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (9). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (10). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1‑mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (11‑13). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (14‑16). CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (17).

References

Schall, T.J. et al. (1990) Nature 347:669.
Bacon, K.B. et al. (1995) Science 269:1727.
Fischer, F.R. et al. (2001) J. Immunol. 167:1637.
Schall, T.J. et al. (1992) Eur. J. Immunol. 22:1477.
Schall, T.J. et al. (1988) J. Immunol. 141:1018.
Appay, V. and S.L. Rowland-Jones (2001) Trends Immunol. 22:83.
Levy, J.A. (2009) J. Immunol. 182:3945.
Randolph-Habecker, J.R. et al. (2002) Cytokine 19:37.
DeVico, A.L. and Gallo, R.C. (2004) Nat. Rev. Microbiol. 2:401.
Proost, P. et al. (1998) J. Biol. Chem. 273:7222.
Appay, V. et al. (1999) J. Biol. Chem. 274:27505.
Proudfoot, A.E.I. et al. (2003) Proc. Natl. Acad. Sci. 100:1885.
von Hundelshausen, P. et al. (2005) Blood 105:924.
von Hundelshausen, P. et al. (2001) Circulation 103:1772.
Zernecke, A. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1897.
Baltus, T. et al. (2003) Blood 102:1985.
Soria, G. and A. Ben-Baruch (2008) Cancer Lett. 267:271.

Entrez Gene IDs

6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)

Alternate Names

CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES

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Citations for Human CCL5/RANTES Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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