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Human Dectin-1/CLEC7A Antibody

Catalog #: AF1859
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1859
AF1859-SP
Product Details
Citations (8)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Dectin-1/CLEC7A Antibody Summary

Species Reactivity
Human
Specificity
Detects human Dectin-1/CLEC7A in direct ELISAs and Western blots. In this format, less than 2% cross‑reactivity with recombinant mouse Dectin-1 and recombinant human Dectin-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Dectin-1/CLEC7A (R&D Systems, Catalog # 1859-DC)
Thr66-Met201
Accession # NP_072092
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<2.0 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Dectin‑1/CLEC7A (Catalog # 1859-DC)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 75 ng/mL of biotinylated Laminarin to immobilized Recombinant Human Dectin-1/CLEC7A (Catalog # 1859-DC) coated at 250 ng/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3-5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5-7). Yeast beta -glucan is accessible to Dectin-1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8 - 10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11-13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15-16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29-39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

References
  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
  3. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
  4. Yokota, K. et al. (2001) Gene 272:51.
  5. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
  6. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
  7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
  8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
  9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
  10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
  11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
  12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
  13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
  14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
  15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
  16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Entrez Gene IDs
64581 (Human); 56644 (Mouse)
Alternate Names
Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1

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Citations for Human Dectin-1/CLEC7A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Glycosphingolipids Mediate Pneumocystis Cell Wall beta -Glucan Activation of the IL-23/IL-17 Axis in Human Dendritic Cells
    Authors: Eva M. Carmona, Theodore J. Kottom, Deanne M. Hebrink, Teng Moua, Raman-Deep Singh, Richard E. Pagano et al.
    American Journal of Respiratory Cell and Molecular Biology
  2. Human Dectin-1 is O-glycosylated and serves as a ligand for C-type lectin receptor CLEC-2
    Authors: S Haji, T Ito, C Guenther, M Nakano, T Shimizu, D Mori, Y Chiba, M Tanaka, SK Mishra, JA Willment, GD Brown, M Nagae, S Yamasaki
    Elife, 2022-12-08;11(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Osteopontin contributes to effective neutrophils recruitment, IL-1? production, and apoptosis in Aspergillus fumigatus keratitis
    Authors: G Zhao, M Hu, C Li, J Lee, K Yuan, G Zhu, C Che
    Immunol. Cell Biol., 2018-02-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Differential susceptibility of Dectin-1 isoforms to functional inactivation by neutrophil and fungal proteases
    Authors: JS Griffiths, A Thompson, M Stott, A Benny, NA Lewis, PR Taylor, J Forton, S Herrick, SJ Orr, EP McGreal
    FASEB J., 2018-01-22;0(0):fj201701145R.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  5. Enhanced immunogenicity of a tricomponent mannan tetanus toxoid conjugate vaccine targeted to dendritic cells via Dectin-1 by incorporating beta-glucan.
    Authors: Lipinski T, Fitieh A, St Pierre J, Ostergaard H, Bundle D, Touret N
    J Immunol, 2013-03-20;190(8):4116-28.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. The modular nature of dendritic cell responses to commensal and pathogenic fungi.
    Authors: Rizzetto, Lisa, Buschow, Sonja I, Beltrame, Luca, Figdor, Carl G, Schierer, Stephan, Schuler, Gerold, Cavalieri, Duccio
    PLoS ONE, 2012-08-03;7(8):e42430.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Protein kinase Cdelta is a critical component of Dectin-1 signaling in primary human monocytes.
    Authors: Elsori DH, Yakubenko VP, Roome T, Thiagarajan PS, Bhattacharjee A, Yadav SP, Cathcart MK
    J. Leukoc. Biol., 2011-06-07;90(3):599-611.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  8. Costimulation of dectin-1 and DC-SIGN triggers the arachidonic acid cascade in human monocyte-derived dendritic cells.
    Authors: Valera I, Fernandez N, Trinidad AG, Alonso S, Brown GD, Alonso A, Crespo MS
    J. Immunol., 2008-04-15;180(8):5727-36.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

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