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Human IGFBP-4 Antibody

Catalog #: AF804
4 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF804
AF804-SP
IGFBP‑4 in Human Placenta.
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Citations (4)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IGFBP-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGFBP‑4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IGFBP-4 is observed, and less than 1% cross-reactivity with recombinant human (rh) IGFBP-1, rhIGFBP-2, rhIGFBP-3, rhIGFBP-5 and rhIGFBP-6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGFBP‑4
Asp22-Glu258
Accession # AAA62670
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IGFBP‑4 (Catalog # 804-GB)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IGFBP‑4 inhibition of IGF‑II-dependent proliferation in the MCF‑7 human breast cancer cell line. Karey, K. P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 12-60 µg/mL in the presence of 0.3 μg/mL Recombinant Human IGFBP‑4 and 14 ng/mL Recombinant Human IGF‑II.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry IGFBP-4 antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

IGFBP‑4 in Human Placenta. IGFBP-4 was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using 5 µg/mL Goat Anti-Human IGFBP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF804) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization IGFBP‑4 Inhibition of IGF‑II-dependent Cell Proliferation and Neutralization by Human IGFBP‑4 Antibody. View Larger

IGFBP‑4 Inhibition of IGF‑II-dependent Cell Proliferation and Neutralization by Human IGFBP‑4 Antibody. Recombinant Human IGFBP-4 (Catalog # 804-GB) inhibits Recombinant Human IGF-II (Catalog # 292-G2) induced proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF-II (14 ng/mL) activity elicited by Recombinant Human IGFBP-4 (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGFBP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF804). The ND50 is typically 12-60 µg/mL.

Immunohistochemistry Detection of Human IGFBP-4 by Immunohistochemistry View Larger

Detection of Human IGFBP-4 by Immunohistochemistry Immunoreactivity (red colour) against IGFBP-4, −5, and PAPPA2 in the syncytiotrophoblast of first trimester placental villi. Serial cross sections of placental villi at 5 and 6 weeks of gestation (7 and 13 weeks not shown) were stained for IGFBP-4, IGFBP-5, and PAPP-A2. Negative controls used non-specific goat IgG in the place of the primary antibodies. FB = fetal blood vessel, CM = chorionic mesoderm, ST = syncytiotrophoblast. Scale bars denote 100 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25475528), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Human IGFBP-4 by Immunohistochemistry View Larger

Detection of Human IGFBP-4 by Immunohistochemistry Immunoreactivity (red colour) against IGFBP-4, −5, and PAPPA2 in the syncytiotrophoblast of first trimester placental villi. Serial cross sections of placental villi at 5 and 6 weeks of gestation (7 and 13 weeks not shown) were stained for IGFBP-4, IGFBP-5, and PAPP-A2. Negative controls used non-specific goat IgG in the place of the primary antibodies. FB = fetal blood vessel, CM = chorionic mesoderm, ST = syncytiotrophoblast. Scale bars denote 100 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25475528), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-4

Human IGF binding protein 4 (IGFBP-4) was isolated from human plasma based on its ability to bind immobilized IGF-I. Human IGFBP-4 cDNA encodes a 258 amino acid (aa) residue precursor protein with a predicted 21 aa residue signal peptide that is processed to generate the 237 aa residue mature human IGFBP-4. The human IGFBP-4 contains a potential N-linked glycosylation site and shares approximately 90% amino acid sequence identity with both the mouse and rat IGFBP-4.

According to the nomenclature of IGFBPs defined at the 4th International Symposium of IGFs (1997, Tokyo), six high-affinity IGF binding proteins
(IGFBP-1, -2, -3, ‑4, ‑5, ‑6), and four IGFBP-related proteins (IGFBPr-1, - 2, -3, -4) have been identified. All IGFBPs have a high cysteine content and share conserved cysteine residues that are clustered in the amino- and carboxy-terminal third of the molecule. IGFBPs have been shown to either inhibit or enhance the biological activities of IGF, or act in an IGF-independent manner. Post-translational modification of IGFBPs, including phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins for IGF and may indirectly regulate IGF actions.

References
  1. Jones, J. I. and D.R. Clemons (1995) Endocrine Rev. 16:3.
  2. Kelly, K.M. et al. (1996) Intl. J. Biochem. Cell Biol. 28:619.
  3. Kim, H-S. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12981.
Long Name
Insulin-like Growth Factor Binding Protein 4
Entrez Gene IDs
3487 (Human); 16010 (Mouse)
Alternate Names
BP-4; HT29-IGFBP; IBP4insulin-like growth factor-binding protein 4; IGF-binding protein 4; IGFBP4; IGFBP-4; IGFBP-4IBP-4; insulin-like growth factor binding protein 4

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Citations for Human IGFBP-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. EWS-FLI-1 creates a cell surface microenvironment conducive to IGF signaling by inducing pappalysin-1
    Authors: P Jayabal, PJ Houghton, Y Shiio
    Genes Cancer, 2017-11-01;8(11):762-770.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells.
    Authors: Crosley, Erin J, Dunk, Caroline, Beristain, Alexande, Christians, Julian K
    Reprod Biol Endocrinol, 2014-12-04;12(0):123.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Fetal baboon sex-specific outcomes in adipocyte differentiation at 0.9 gestation in response to moderate maternal nutrient reduction.
    Authors: Tchoukalova Y, Krishnapuram R, White U, Burk D, Fang X, Nijland M, Nathanielsz P
    Int J Obes (Lond), 2013-06-10;38(2):224-30.
    Species: Primate - Papio cynocephalus (Yellow Baboon)
    Sample Types: Whole Cells
    Applications: Western Blot
  4. The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
    Authors: McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A
    Gastroenterology, 2006-03-06;130(6):1754-63.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human IGFBP-4 Antibody
By Natalie Haywood on 04/10/2019
Application: WB Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human