Human MMP-2 Antibody

Name: Human MMP-2 Antibody
Brand: R&D Systems
SKU: AF902
Rating: 4.3 (4 reviews)

Catalog #: AF902
21 Citations 4 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF902
AF902-SP

Detection of Human MMP‑2 by Western Blot.

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All MMP-2 Antibodies

Product Details

Citations (21)

FAQs

Supplemental Products

Reviews (4)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MMP-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MMP-2 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human MMP‑2
Ile34-Cys660
Accession # P08253

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human MMP‑2 (Catalog # 902-MP), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human MMP‑2 by Western Blot. Western blot shows lysate of U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for MMP-2 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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MMP‑2 in Human Ovarian Cancer Tissue. MMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human MMP-2 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF902) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry

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MMP‑2 in Human Ovary. MMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian array using Goat Anti-Human MMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western

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Detection of Human MMP‑2 by Simple Western^TM. Simple Western lane view shows lysates of U-118-MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for MMP-2 at approximately 78 kDa (as indicated) using 10 µg/mL of Goat Anti-Human MMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-2

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites.

Long Name

Matrix Metalloproteinase 2

Entrez Gene IDs

4313 (Human); 17390 (Mouse)

Alternate Names

72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human MMP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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Schwann Cell Stimulation of Pancreatic Cancer Cells: A Proteomic Analysis
Authors: Aysha Ferdoushi, Xiang Li, Nathan Griffin, Sam Faulkner, M. Fairuz B. Jamaluddin, Fangfang Gao et al.
Frontiers in Oncology
A systematic review and critical evaluation of immunohistochemical associations in hidradenitis suppurativa.
Authors: Frew John W, Hawkes Jason E, Krueger James G
F1000Research
Tissue- and Cell-Specific Co-localization of Intracellular Gelatinolytic Activity and Matrix Metalloproteinase 2.
Authors: Solli AI, Fadnes B, Winberg JO et al.
J Histochem Cytochem
Elevated Serum Gastrin Is Associated with Melanoma Progression: Putative Role in Increased Migration and Invasion of Melanoma Cells
Authors: Varga, AJ;Nemeth, IB;Kemeny, L;Varga, J;Tiszlavicz, L;Kumar, D;Dodd, S;Simpson, AWM;Buknicz, T;Beynon, R;Simpson, D;Krenacs, T;Dockray, GJ;Varro, A;
International journal of molecular sciences
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot
Gold Nanoparticles Inhibit Extravasation of Canine Osteosarcoma Cells in the Ex Ovo Chicken Embryo Chorioallantoic Membrane Model
Authors: Ma?ek, A;Wojnicki, M;Borkowska, A;Wójcik, M;Zió?ek, G;Lechowski, R;Zabielska-Koczyw?s, K;
International journal of molecular sciences
Species: Canine
Sample Types: Cell Lysates
Applications: Western Blot
Protease-dependent defects in N-cadherin processing drive PMM2-CDG pathogenesis
Authors: EJ Klaver, L Dukes-Rims, B Kumar, ZJ Xia, T Dang, MA Lehrman, P Angel, RR Drake, HH Freeze, R Steet, H Flanagan-S
JCI Insight, 2021-12-22;0(0):.
Species: Zebrafish
Sample Types: Tissue Homogenates
Applications: Western Blot
TIMP-2 regulates proliferation, invasion and STAT3-mediated cancer stem cell-dependent chemoresistance in ovarian cancer cells
Authors: RM Escalona, M Bilandzic, P Western, E Kadife, G Kannouraki, JK Findlay, N Ahmed
BMC Cancer, 2020-10-06;20(1):960.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Overexpression of microRNA-367 inhibits angiogenesis in ovarian cancer by downregulating the expression of LPA1
Authors: Q Zheng, X Dai, W Fang, Y Zheng, J Zhang, Y Liu, D Gu
Cancer Cell Int, 2020-10-02;20(0):476.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Glycosaminoglycans influence enzyme activity of MMP2 and MMP2/TIMP3 complex formation - Insights at cellular and molecular level
Authors: G Ruiz-Gómez, S Vogel, S Möller, MT Pisabarro, U Hempel
Sci Rep, 2019-03-20;9(1):4905.
Species: Human
Sample Types: Whole Cells
Applications: ICC
EDTA/gelatin zymography method to identify C1s versus activated MMP-9 in plasma and immune complexes of patients with systemic lupus erythematosus
Authors: E Ugarte-Ber, E Martens, L Boon, J Vandooren, D Blockmans, P Proost, G Opdenakker
J. Cell. Mol. Med., 2018-10-24;23(1):576-585.

Age-related lung tissue remodeling due to the local distribution of MMP-2, TIMP-2, TGF-? and Hsp70
Authors: Z Vitenberga, M Pilmane
Biotech Histochem, 2018-01-12;0(0):1-10.
Species: Human
Sample Types: Whole Tissue

Humanin, a cytoprotective peptide, is expressed in carotid atherosclerotic [corrected] plaques in humans.
Authors: Zacharias DG, Kim SG, Massat AE, Bachar AR, Oh YK, Herrmann J, Rodriguez-Porcel M, Cohen P, Lerman LO, Lerman A
PLoS ONE, 2012-02-06;7(2):e31065.
Species: Human
Sample Types: Whole Cells
Applications: ICC

ErbB2-enhanced invasiveness of H-Ras MCF10A breast cells requires MMP-13 and uPA upregulation via p38 MAPK signaling.
Authors: Yong HY, Kim IY, Kim JS, Moon A
Int. J. Oncol., 2010-02-01;36(2):501-7.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot

Fibroblast-conditioned media promote human sarcoma cell invasion.
Authors: Bittner JG, Wilson M, Shah MB, Albo D, Feig BW, Wang TN
Surgery, 2008-09-14;145(1):42-7.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008-04-19;7(6):2406-14.
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development

Claudin-1 overexpression in melanoma is regulated by PKC and contributes to melanoma cell motility.
Authors: Leotlela PD, Wade MS, Duray PH, Rhode MJ, Brown HF, Rosenthal DT, Dissanayake SK, Earley R, Indig FE, Nickoloff BJ, Taub DD, Kallioniemi OP, Meltzer P, Morin PJ, Weeraratna AT
Oncogene, 2006-12-11;26(26):3846-56.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Differential expression and activity of matrix metalloproteinases during flow-modulated vein graft remodeling.
Authors: Berceli SA, Jiang Z, Klingman NV, Pfahnl CL, Abouhamze ZS, Frase CD, Schultz GS, Ozaki CK
J. Vasc. Surg., 2004-05-01;39(5):1084-90.
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHC-P

Complex Evaluation of Tissue Factors in Pediatric Cholesteatoma
Authors: Kristaps Dambergs, Gunta Sumeraga, Māra Pilmane
Children (Basel)

Inhibition of Tyrosine Kinase Receptor Tie2 Reverts HCV-Induced Hepatic Stellate Cell Activation
Authors: Samuel Martín-Vílchez, Yolanda Rodríguez-Muñoz, Rosario López-Rodríguez, Ángel Hernández-Bartolomé, María Jesús Borque-Iñurrita, Francisca Molina-Jiménez et al.
PLoS ONE

Glucocorticoids indirectly decrease colon cancer cell proliferation and invasion via effects on cancer-associated fibroblasts
Authors: Zuzanna Drebert, Elly De Vlieghere, Jolien Bridelance, Olivier De Wever, Karolien De Bosscher, Marc Bracke et al.
Experimental Cell Research

Tumour Necrosis Factor-alpha and Matrix Metalloproteinase-2 are Expressed Strongly in Hidradenitis Suppurativa
Authors: Elga Mozeika, Mara Pilmane, Birgit Meinecke Nürnberg, Gregor B E Jemec
Acta Dermato Venereologica