Human/Mouse ERK5/BMK1 Antibody Summary
Met1-Glu432
Accession # Q13164
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse ERK5/BMK1 by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse ERK5/BMK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2848) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ERK5/BMK1 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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ERK5/BMK1 in HeLa Human Cell Line. ERK5/BMK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse ERK5/BMK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2848) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ERK5/BMK1
Extracellular signal-regulated kinase 5 (ERK5), also known as big mitogen-activated protein kinase 1 (BMK1) and MAPK7, is activated by a several mechanisms, including receptor tyrosine kinases, G protein-coupled receptors, and osmotic stress. Like ERK1 and ERK2, ERK5 contains the conserved Thr-Glu-Tyr activation motif in its activation loop. Unlike these ERKs, however, ERK5 contains a unique C-terminal domain that regulates its activation and nuclear translocation. ERK5’s upstream MAPK kinase has been identified as MEK5.
Product Datasheets
Citations for Human/Mouse ERK5/BMK1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Memantine protects against ischemia/reperfusion-induced brain endothelial permeability
Authors: Y Liu, Y Huang, Y Xu, P Qu, M Wang
IUBMB Life, 2018-03-15;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Statin regulated ERK5 stimulates tight junction formation and reduces permeability in human cardiac endothelial cells
Authors: Emma L. Wilkinson, James E. Sidaway, Michael J. Cross
Journal of Cellular Physiology
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Erk5 is a mediator to TGFbeta1-induced loss of phenotype and function in human podocytes.
Authors: Badshah I, Baines D, Dockrell M
Front Pharmacol, 2014-04-21;5(0):71.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
TGF-beta activates ERK5 in human renal epithelial cells.
Authors: Browne JA, Pearson AL, Zahr RA, Niculescu-Duvaz I, Baines DL, Dockrell ME
Biochem. Biophys. Res. Commun., 2008-06-25;373(3):440-4.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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