Human/Mouse PRMT1 Antibody Summary
Lys218-Arg361
Accession # Q99873
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse PRMT1 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line and Hepa 1-6 mouse hepatoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human PRMT1 Polyclonal Antibody (Catalog # AF6016) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PRMT1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PRMT1
PRMT/PRMT1 (Protein-Arginine Methyltransferase 1; also HRMT1L2 and HMT2) is a 42‑45 kDa type I member of the arginine N‑methyltransferase family of enzymes. It is ubiquitously expressed and dimethylates single arginine residue nitrogen atoms in a distributive manner (i.e.-with substrate release following each methlyation step). PRMT1 forms homodimers, and heterodimers with PRMT3, and exists as part of a 300‑400 kDa complex in vivo. Human PRMT1 is 361 amino acids in length and contains one adenosyl-L-methionine binding site (aa 85‑153) and a phosphorylation site at Tyr299. There are multiple splice variants that show distinct substrate specificities. There is an alternate start site 10 aa upstream of the standard start site, and a single Met, plus a five aa and 11 aa substiuition for aa 1‑19. One isoform has a 2 aa substitution for aa 1‑20 accompanied by a deketion of aa 52‑67. Over aa 218‑361, human PRMT1 is 100% identical in aa sequence to mouse PRMT1.
Product Datasheets
Citations for Human/Mouse PRMT1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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A super-enhancer-regulated RNA-binding protein cascade drives pancreatic cancer
Authors: Corina E. Antal, Tae Gyu Oh, Stefan Aigner, En-Ching Luo, Brian A. Yee, Tania Campos et al.
Nat Commun
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L-Arginine/Nitric Oxide Pathway Is Altered in Colorectal Cancer and Can Be Modulated by Novel Derivatives from Oxicam Class of Non-Steroidal Anti-Inflammatory Drugs
Authors: M Krzystek-K, B Szcz??niak, I Szczuka, P Fortuna, M Zawadzki, A Kubiak, M Mierzcha?a, MG Fleszar, ? Lewandowsk, P Serek, N Jamrozik, K Neubauer, J Wi?niewski, R Kempi?ski, W Witkiewicz, I Bednarz-Mi
Cancers (Basel), 2020-09-11;12(9):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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