Human/Rat Cortactin Antibody

Catalog #: AF6096 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6096
AF6096-SP

Detection of Human and Rat Cortactin by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat Cortactin Antibody Summary

Species Reactivity

Human, Rat

Specificity

Detects human and rat Cortactin in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Cortactin
Met1-Gly85
Accession # Q14247

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Knockout Validated

Cortactin is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Cortactin knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Rat Cortactin by Western Blot. Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, MDA-MB-453 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Rat Cortactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6096) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Cortactin at approximately 80-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated

Western Blot Shows Human Cortactin Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human Cortactin Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Cortactin knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Rat Cortactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6096) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cortactin at approximately 80 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cortactin

Cortactin (also EMS1 and Amplaxin) is an 80‑85 kDa multidomain scaffold-associated protein. It is an actin-binding cytoplasmic protein found at intercellular junctions and near lamellipodia. It is also found in tumor invadopodia, a somewhat analogous structure to lamellipodia. Here, Cortactin appears to coordinate actin assembly with MMP secretion, thus facilitating tumor invasiveness. Human Cortactin is 550 amino acids (aa) in length and contains six-plus Cortactin regions (aa 80‑324) that regulate actin polymerization, and one SH3 domain (aa 496‑548) that binds to N-WASP. Cortactin is highly phosphorylated on Tyr and Ser/Thr, and undergoes acetylation.

Entrez Gene IDs

2017 (Human); 13043 (Mouse); 60465 (Rat)

Alternate Names

1110020L01Rik; Amplaxin; Cortactin; CTTN; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 srcsubstrate); EMS1; EMS1amplaxin; FLJ34459; Oncogene EMS1; src substrate cortactin