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Human S100B Antibody

Catalog #: AF1820
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1820
AF1820-SP
S100B in Human Alzheimer'S Brain.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human S100B Antibody Summary

Species Reactivity
Human
Specificity
Detects human S100B in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) S100A1, rhS100A4, and rhS100A6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human S100B
Met1-Glu92
Accession # P04271
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human S100B
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry S100B antibody in Human Alzheimer's Brain by Immunohistochemistry (IHC-P). View Larger

S100B in Human Alzheimer'S Brain. S100B was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Goat Anti-Human S100B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1820) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human S100B antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human S100B by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for S100B at approximately 4 kDa (as indicated) using 10 µg/mL of Goat Anti-Human S100B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1820). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100B

S100B belongs to the S100 subgroup of the EF-hand family of Ca2+ binding proteins. It is a homodimer that is expressed primarily in the brain by astrocytes, oligodendrocytes and Schwann cells. S100B has multiple intracellular functions, but can also be secreted from cells to exert extracellular functions. Some of the extracellular functions of S100B may be mediated by RAGE (receptor for advanced glycation end products). Blood levels of S100B can be used to monitor the extent of brain injury and malignant melanoma.

Long Name
S100 Calcium Binding Protein B
Entrez Gene IDs
6285 (Human)
Alternate Names
beta (neural); NEF; S100 beta; S100 calcium binding protein B; S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S-100 calcium-binding protein, beta chain, 10protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100; S100B; S100beta

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Citations for Human S100B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Astrocyte-derived MFG-E8 facilitates microglial synapse elimination in Alzheimer's disease mouse models
    Authors: Sokolova, D;Ghansah, SA;Puletti, F;Georgiades, T;De Schepper, S;Zheng, Y;Crowley, G;Wu, L;Rueda-Carrasco, J;Koutsiouroumpa, A;Muckett, P;Freeman, OJ;Khakh, BS;Hong, S;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Zinc finger and SCAN domain-containing 18 suppresses the proliferation, self-renewal, and drug resistance of glioblastoma cells
    Authors: Wang, Y;Peng, J;Song, C;Yang, Y;Qin, T;
    Heliyon
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. PLD3 affects axonal spheroids and network defects in Alzheimer's disease
    Authors: P Yuan, M Zhang, L Tong, TM Morse, RA McDougal, H Ding, D Chan, Y Cai, J Grutzendle
    Nature, 2022-11-30;612(7939):328-337.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Disease Progression-Dependent Effects of TREM2 Deficiency in a Mouse Model of Alzheimer's Disease.
    Authors: Jay T, Hirsch A, Broihier M, Miller C, Neilson L, Ransohoff R, Lamb B, Landreth G
    J Neurosci, 2017-01-18;37(3):637-647.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Increased serum S100B levels in chronic schizophrenic patients on long-term clozapine or typical antipsychotics.
    Authors: Qi LY, Xiu MH, Chen da C, Wang F, Kosten TA, Kosten TR, Zhang XY
    Neurosci. Lett., 2009-06-17;462(2):113-7.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  6. Glial Cell Line-derived Neurotrophic Factor-overexpressing Human Neural Stem/Progenitor Cells Enhance Therapeutic Efficiency in Rat with Traumatic Spinal Cord Injury
    Authors: Kyujin Hwang, Kwangsoo Jung, Il-Sun Kim, Miri Kim, Jungho Han, Joohee Lim et al.
    Experimental Neurobiology

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