Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Knockout Validated, Immunohistochemistry, Western Blot, Neutralization, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Proximity Ligation Assay, In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TIMP-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TIMP-1
Cys24-Ala207
Accession # Q6FGX5

Specificity

Detects human TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse TIMP‑1 and recombinant rat TIMP-1 is observed and less than 1% cross-reactivity with recombinant human (rh) TIMP-2, rhTIMP-3, and rhTIMP‑4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human TIMP‑1 Antibody

Detection of Human TIMP-1 antibody by Western Blot.

Detection of Human TIMP‑1 by Western Blot.

Western blot shows lysates of human lung tissue and human prostate tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP-1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human TIMP-1 antibody by Western Blot.

Detection of Human TIMP‑1 by Western Blot.

Western blot shows lysates of PC-3 human prostate cancer cell line, HT-29 human colon adenocarcinoma cell line, and SK-OV-3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 5 µg/mL of Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP-1 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TIMP-1 antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P).

TIMP‑1 in Human Colon Cancer Tissue.

TIMP-1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and extracellular space. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human TIMP-1 antibody by Simple WesternTM.

Detection of Human TIMP‑1 by Simple WesternTM.

Simple Western lane view shows lysates of PC-3 human prostate cancer cell line, HT-29 human colon adenocarcinoma cell line, and human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for TIMP-1 at approximately 42-45 kDa (as indicated) using 50 µg/mL of Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human TIMP-1 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human TIMP‑1 Specificity by Using Knockout Cell Line.

Western blot shows lysates of SK-OV-3 human ovarian adenocarcinoma cell line and TIMP-1 knockout SK-OV-3 cell line (KO). PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP-1 at approximately 25 kDa (as indicated) in the parental SK-OV-3 cell line, but is not detectable in knockout SK-OV-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization of TIMP‑1 Activity by Human TIMP‑1 Antibody.

Neutralization of TIMP‑1 Activity by Human TIMP‑1 Antibody.

Recombinant Human MMP-2 (0.2 µg/mL, Catalog # 902-MP) activity is measured in the presence of Recombinant Human TIMP-1 (0.1 µg/mL, Catalog # 970-TM) that has been preincubated with increasing concentrations of Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970). The ND50 is typically 1 µg/mL.

Detection of Human TIMP-1 by Immunohistochemistry-Paraffin

Detection of Human TIMP-1 by Immunohistochemistry-Paraffin

IHC staining result investigating TR4 level and macrophage infiltration in tumor tissues of PCa patients, as well as TIMP-1/MMP2/MMP9 signaling. IHC staining was performed using TR4 antibody (1:300),CD68 antibody (1:200),TIMP-1 antibody (1:200),MMP2 antibody (1:200),MMP9 antibody (1:200). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25623427), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human TIMP-1 by Proximity Ligation Assay

Detection of Human TIMP-1 by Proximity Ligation Assay

Univariate survival analysis of disease free survival (DFS) in plasma for all 465 patients. A) MMP-9:TIMP-1 measured by ELISA. B) MMP-9:TIMP-1 measured by PLA. Patients are divided into four groups of equal size (Q1-Q4) according to increasing plasma MMP-9:TIMP-1 levels; Q1 being the group with the lowest level. Image collected and cropped by CiteAb from the following open publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-598), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human TIMP-1 by Proximity Ligation Assay

Detection of Human TIMP-1 by Proximity Ligation Assay

Univariate survival analysis of disease free survival (DFS) in plasma for all 465 patients. A) MMP-9:TIMP-1 measured by ELISA. B) MMP-9:TIMP-1 measured by PLA. Patients are divided into four groups of equal size (Q1-Q4) according to increasing plasma MMP-9:TIMP-1 levels; Q1 being the group with the lowest level. Image collected and cropped by CiteAb from the following open publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-598), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TIMP‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue

Knockout Validated

TIMP‑1 is specifically detected in SK‑OV‑3 human ovarian adenocarcinoma parental cell line but is not detectable in TIMP‑1 knockout SK‑OV‑3 cell line.

Simple Western

50 µg/mL
Sample: PC‑3 human prostate cancer cell line, HT‑29 human colon adenocarcinoma cell line, and human lung tissue

Western Blot

1-5 µg/mL
Sample: Human lung tissue, human prostate tissue, PC‑3 human prostate cancer cell line, HT‑29 human colon adenocarcinoma cell line, and SK‑OV‑3 human ovarian adenocarcinoma cell line

Neutralization

Measured by its ability to neutralize Recombinant Human TIMP-1 (0.1 µg/mL, Catalog # 970-TM) inhibition of Recombinant Human MMP‑2 (0.2 µg/mL, Catalog # 902-MP) cleavage of the fluorogenic peptide substrate Mca‑PLGL-Dpa-AR-NH2 (5 µM, Catalog # ES001). The Neutralization Dose (ND50) is typically 1 µg/mL.

Reviewed Applications

Read 3 reviews rated 4.3 using AF970 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIMP-1

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with Pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.

Long Name

Tissue Inhibitors of Metalloproteinases 1

Alternate Names

TIMP1

Entrez Gene IDs

7076 (Human); 21857 (Mouse); 116510 (Rat)

Gene Symbol

TIMP1

UniProt

Q6FGX5

Additional TIMP-1 Products

Product Documents for Human TIMP‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TIMP‑1 Antibody

For research use only

Citations for Human TIMP‑1 Antibody

Customer Reviews for Human TIMP‑1 Antibody (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human TIMP-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: mesenchymal stem cells derived extracellular vesicles
    Species: Human
    Verified Customer | Posted 03/21/2022
    Negative control: murine macrophages. MSC-EV: mesenchymal stem cells derived extracellular vesicles
    Human TIMP‑1 Antibody AF970
  • Human TIMP-1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Frozen human skin sections
    Species: Human
    Verified Customer | Posted 07/18/2019
    TIMP1 (green) was detected in human skin (melanoma) using TIMP1 antibody (1:40; 1 h) and secondary anti-goat-AlexaFluor488 (1:400; 30 min) in PBS. Nuclei were stained with Dapi (blue).
    Human TIMP‑1 Antibody AF970
  • Human TIMP-1 Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2017

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