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Mouse CCL24/Eotaxin-2/MPIF-2 Antibody

Catalog #: AF528
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF528
AF528-SP
Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutralization by Mouse CCL24/Eotaxin‑2 Antibody.
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Citations (7)
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Mouse CCL24/Eotaxin-2/MPIF-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects CCL24/Eotaxin‑2/MPIF‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human Eotaxin-2 is observed, and less than 1% cross-reactivity with recombinant mouse Eotaxin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CCL24/Eotaxin‑2/MPIF‑2
Ile24-Val119
Accession # Q9JKC0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2 (Catalog # 528-MP)
Neutralization
Measured by its ability to neutralize CCL24/Eotaxin‑2/MPIF‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 0.02 µg/mL Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutralization by Mouse CCL24/Eotaxin‑2 Antibody. View Larger

Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutralization by Mouse CCL24/Eotaxin‑2 Antibody. Recombinant Mouse CCL24/Eotaxin-2 (Catalog # 1017-MP) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL24/Eotaxin-2 (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL24/ Eotaxin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF528). The ND50 is typically 0.5-2.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL24/Eotaxin-2/MPIF-2

Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) residue precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to eotaxin/CCL11 and Eotaxin-3/CCL26. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by eotaxin.

Entrez Gene IDs
6369 (Human); 56221 (Mouse)
Alternate Names
CCL24; chemokine (C-C motif) ligand 24; Ckb-6; Eosinophil chemotactic protein 2; Eotaxin-2; member 24; MPIF2; MPIF-2; MPIF2Myeloid progenitor inhibitory factor 2; MPIF-2Small-inducible cytokine A24; SCYA24CK-beta-6

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Citations for Mouse CCL24/Eotaxin-2/MPIF-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Alendronate Attenuates Eosinophilic Airway Inflammation Associated with Suppression of Th2 Cytokines, Th17 Cytokines, and Eotaxin-2
    Authors: Oh Sasaki, Mitsuru Imamura, Yusuke Yamazumi, Hiroaki Harada, Taku Matsumoto, Katsuhide Okunishi et al.
    The Journal of Immunology
  2. CD200R signaling contributes to unfavorable tumor microenvironment through regulating production of chemokines by tumor-associated myeloid cells
    Authors: Cho-Hao Lin, Fatemeh Talebian, Yang Yang, Jianmin Zhu, Jin-Qing Liu, Bolin Zhao et al.
    iScience
  3. TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus
    Authors: Y Kannan, LJ Entwistle, VS Pelly, J Perez-Llor, AW Walker, SC Ley, MS Wilson
    PLoS Pathog., 2017-07-31;13(7):e1006536.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. Macrophages and cardiac fibroblasts are the main producers of eotaxins and regulate eosinophil trafficking to the heart
    Eur J Immunol, 2016-10-25;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. CCR3 is a target for age-related macular degeneration diagnosis and therapy.
    Authors: Takeda A, Baffi JZ, Kleinman ME, Cho WG, Nozaki M, Yamada K, Kaneko H, Albuquerque RJ, Dridi S, Saito K, Raisler BJ, Budd SJ, Geisen P, Munitz A, Ambati BK, Green MG, Ishibashi T, Wright JD, Humbles AA, Gerard CJ, Ogura Y, Pan Y, Smith JR, Grisanti S, Hartnett ME, Rothenberg ME, Ambati J
    Nature, 2009-06-14;460(7252):225-30.
    Species: Mouse
    Sample Types: Vitreous Humor
    Applications: Neutralization
  7. A20-binding inhibitor of NF-kappa B (ABIN) 2 negatively regulates allergic airway inflammation
    Authors: Sonia Ventura, Florencia Cano, Yashaswini Kannan, Felix Breyer, Michael J. Pattison, Mark S. Wilson et al.
    Journal of Experimental Medicine

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