Mouse CD24 Antibody Summary
Accession # P24807
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD24 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse CD24 Monoclonal Antibody (Catalog # MAB8547) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
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CD24 in Mouse Stomach. CD24 was detected in immersion fixed paraffin-embedded sections of mouse stomach using Rat Anti-Mouse CD24 Monoclonal Antibody (Catalog # MAB8547) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to parietal cells in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD24
CD24, also known as Heat Stable Antigen (HSA), is a heavily and variably glycosylated 35-60 kDa GPI-linked sialoprotein. CD24 is expressed on B cells, T cells, keratinocytes, and myofiber synaptic nuclei and is upregulated in a wide variety of cancers. It is also a marker for exosomes released into the urine and amniotic fluid. CD24 binds to P-Selectin on activated platelets and vascular endothelial cells. CD24 ligation induces tumor cell apoptosis and is important for the deletion of autoreactive T cells. Mature mouse CD24 shares 30% aa sequence identity with human CD24.
Product Datasheets
Citations for Mouse CD24 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Urine-Derived Renal Epithelial Cells (URECs) from Transplanted Kidneys as a Promising Immunomodulatory Cell Population
Authors: Pizzuti, V;Donadei, C;Balducelli, E;Conte, D;Gessaroli, E;Paris, F;Bini, C;Demetri, M;Di Nunzio, M;Corradetti, V;Alviano, F;La Manna, G;Comai, G;
Cells
Species: Human
Sample Types: Whole Cells
Applications: ICC -
HIV-1 Nef down-modulates C-C and C-X-C chemokine receptors via ubiquitin and ubiquitin-independent mechanism.
Authors: Chandrasekaran, Prabha, Moore, Victoria, Buckley, Monica, Spurrier, Joshua, Kehrl, John H, Venkatesan, Sundarar
PLoS ONE, 2014-01-29;9(1):e86998.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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