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Mouse CD69 Antibody

Catalog #: MAB2386
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2386
MAB2386-SP
Product Details
Citations (1)
FAQs
Supplemental Products
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD69 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD69 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD69 is observed.
Source
Monoclonal Rat IgG2B Clone # 310106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD69
Gly64-Arg199
Accession # P37217
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CD69 under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
CTLL‑2 mouse cytotoxic T cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD69

CD69, also known as Leu 23, AIM, EA-1 and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells and platelets.

Entrez Gene IDs
969 (Human); 12515 (Mouse)
Alternate Names
activation inducer molecule (AIM/CD69); Activation inducer molecule; AIM; BL-AC/P26; CD69 antigen (p60, early T-cell activation antigen); CD69 antigen; CD69 molecule; CD69; CLEC2CC-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; EA-1; early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leu23; Leukocyte surface antigen Leu-23; MLR-3; p60

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Product Datasheet
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Citation for Mouse CD69 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Rai acts as a negative regulator of autoimmunity by inhibiting antigen receptor signaling and lymphocyte activation.
    Authors: Savino MT, Ortensi B, Ferro M, Ulivieri C, Fanigliulo D, Paccagnini E, Lazzi S, Osti D, Pelicci G, Baldari CT
    J. Immunol., 2009-01-01;182(1):301-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Affinity Purification

FAQs

  1. What is the light chain isotype for Catalog # MAB2386?

    • The light chain isotype of  Catalog # MAB2386 is kappa.

  2. What is the light chain of the Mouse CD69 Antibody, Catalog #s MAB2386 and FAB2386, Clone # 1034124?

    • Catalog #s MAB2386 and FAB2386 have a kappa light chain.

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