Mouse FGFR3 Antibody

Catalog #: MAB710
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB710
MAB710-SP

FGF R3 Inhibition of FGF acidic-dependent Cell Proliferation and Neutral-ization by Mouse FGF R3 Antibody.

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse FGFR3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse FGF R3 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with the (IIIb) and (IIIc) isoforms of recombinant human FGF R3 is observed. Neutralizes the bioactivity of mouse FGF R3. Functions equally as well in the neutralization of recombinant mouse (rm) FGF R3 alpha (IIIb) and rmFGF R3 alpha (IIIc).

Source

Monoclonal Rat IgG_2A Clone # 136812

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse FGF R3
Glu21-Tyr369 (Asn310Ile, Ala354 del) (predicted)
Accession # NP_001156689

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize FGF R3-mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 3-12 µg/mL in the presence of 12 ng/mL Recombinant Mouse FGF R3 (IIIc) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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FGF R3 Inhibition of FGF acidic-dependent Cell Proliferation and Neutral-ization by Mouse FGF R3 Antibody. Recombinant Mouse FGF R3 (IIIc) Fc Chimera (Catalog # 710-MF) inhibits Recom-binant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recom-binant Human FGF acidic (0.3 ng/mL) activity elicited by Recom-binant Mouse FGF R3 (IIIc) Fc Chimera (12 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse FGF R3 Mono-clonal Antibody (Catalog # MAB710). The ND₅₀ is typically 3-12 µg/mL in the presence of heparin (10 µg/mL).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR3

Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGF R1‑4, are known. All four genes for FGF Rs encode proteins with an N‑terminal signal peptide, three immunoglobulin (Ig)‑like domains, an acid‑box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine‑kinase domain. Multiple forms of FGF R1‑3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform. Only the alpha isoform has been identified for FGF R3 and FGF R4. Additional splicing events for FGF R1‑3, involving the C‑terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). A IIIa isoform which is a secreted FGF binding protein containing only the N‑terminal half of the IgIII domain plus some intron sequences has also been reported for FGF R1. Mutations in FGF R1‑3 have been found in patients with birth defects involving craniosynostosis. The complex patterns of expression of these receptors as well as the specificity of their interactions with the various FGF ligand family members are being investigated.

References

Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
Burke, D. et al. (1998) Trends Biochem. Sci. 23:59.

Long Name

Fibroblast Growth Factor Receptor 3

Entrez Gene IDs

2261 (Human); 14184 (Mouse)

Alternate Names

CD333; CEK; EC 2.7.10; FGF R3; FGFR3; fibroblast growth factor receptor 3; HSFGFR3EX; JTK4; JTK4thanatophoric dwarfism

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Citations for Mouse FGFR3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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