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Mouse IL-6 Antibody

Catalog #: AF-406-NA
48 Citations 6 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-406-NA
AF-406-SP
Detection of Mouse IL‑6 by Western Blot.
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Product Details
Citations (48)
FAQs
Supplemental Products
Reviews (6)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-6 in direct ELISAs and Western blots.  In direct ELISAs, approximately 100% cross-reactivity with recombinant rat IL‑6 and recombinant cotton rat IL-6 is observed and less than 15% cross-reactivity with recombinant human IL-6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL-6
Phe25-Thr211
Accession # P08505
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
Immersion fixed mouse splenocytes treated with PMA and ionomycin
Neutralization
Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND50) is typically 0.01-0.03 µg/mL in the presence of 0.25 ng/mL Recombinant Mouse IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IL-6 antibody by Western Blot. View Larger

Detection of Mouse IL‑6 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry IL-6 antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr). View Larger

IL‑6 in Mouse Thymus. IL-6 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Neutralization Cell Proliferation Induced by IL‑6 and Neutralization by Mouse IL‑6 Antibody. View Larger

Cell Proliferation Induced by IL‑6 and Neutralization by Mouse IL‑6 Antibody. Recombinant Mouse IL-6 (Catalog # 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-406-NA). The ND50 is typically 0.01-0.03 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature mouse IL-6 is 187 amino acids (aa) in length that is typically expressed as a 22-28 kDA molecular weight protein. Mouse IL-6 shares 39% and 85% aa sequence identity with human and rat IL-6, respectively. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Long Name
Interleukin 6
Entrez Gene IDs
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names
B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

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Citations for Mouse IL-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

48 Citations: Showing 1 - 10
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  1. Blockade of IL-6 signaling alleviates atherosclerosis in Tet2-deficient clonal hematopoiesis
    Authors: Wenli Liu, Mustafa Yalcinkaya, Inés Fernández Maestre, Malgorzata Olszewska, Patrick B. Ampomah, J. Brett Heimlich et al.
    Nature Cardiovascular Research
  2. DAPT, a potent Notch inhibitor regresses actively growing abdominal aortic aneurysm via divergent pathways
    Authors: Chetan P. Hans, Neekun Sharma, Rishabh Dev, Jones M. Blain, Jeff Tonniges, Gunjan Agarwal
    Clinical Science
  3. EphA2 Is a Clinically Relevant Target for Breast Cancer Bone Metastatic Disease
    Authors: David B Vaught, Alyssa R Merkel, Conor C Lynch, James Edwards, Mohammed Noor Tantawy, Timothy Hilliard et al.
    JBMR Plus
  4. TRAF3IP2 mediates aldosterone/salt-induced cardiac hypertrophy and fibrosis.
    Authors: Sakamuri SS, Valente AJ, Siddesha JM et al.
    Mol. Cell. Endocrinol.
  5. Oestrogen promotes healing in a bacterial LPS model of delayed cutaneous wound repair
    Authors: Rachel Crompton, Helen Williams, David Ansell, Laura Campbell, Kirsty Holden, Sheena Cruickshank et al.
    Laboratory Investigation
  6. I kappa B epsilon Is a Key Regulator of B Cell Expansion by Providing Negative Feedback on cRel and RelA in a Stimulus-Specific Manner
    Authors: Bryce N. Alves, Rachel Tsui, Jonathan Almaden, Maxim N. Shokhirev, Jeremy Davis-Turak, Jessica Fujimoto et al.
    The Journal of Immunology
  7. Covalently grafted human serum albumin coating mitigates the foreign body response against silicone implants in mice
    Authors: Zhou, X;Hao, H;Chen, Y;Cao, W;Zhu, Z;Ni, Y;Liu, Z;Jia, F;Wang, Y;Ji, J;Peng Zhang, ;
    Bioactive materials
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. MTHFD2 reprograms macrophage polarization by inhibiting PTEN
    Authors: Shang, M;Ni, L;Shan, X;Cui, Y;Hu, P;Ji, Z;Shen, L;Zhang, Y;Zhou, J;Bing Chen, ;Wang, T;Yu, Q;
    Cell reports
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. The study of direct and indirect effects of radiofrequency ablation on tumor microenvironment in liver tumor animal model
    Authors: AN Jiang, B Wang, S Wang, K Zhao, H Wu, K Yan, W Wu, W Yang
    BMC Cancer, 2022-06-17;22(1):663.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Effect of treatment with conditioned media derived from C2C12 myotube on adipogenesis and lipolysis in 3T3-L1 adipocytes
    Authors: K Tamura, N Goto-Inoue, K Miyata, Y Furuichi, NL Fujii, Y Manabe
    PLoS ONE, 2020-08-05;15(8):e0237095.
    Species: Mouse
    Sample Types: Cell Culture Lysates, Cell Lysates
    Applications: Western Blot
  11. Targeted Disruption of Bone Marrow Stromal Cell-Derived Gremlin1 Limits Multiple Myeloma Disease Progression In Vivo
    Authors: KC Clark, DR Hewett, V Panagopoul, N Plakhova, KS Opperman, AL Bradey, KM Mrozik, K Vandyke, S Mukherjee, GCG Davies, DL Worthley, ACW Zannettino
    Cancers (Basel), 2020-08-03;12(8):.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  12. The microRNA-34a-Induced Senescence-Associated Secretory Phenotype (SASP) Favors Vascular Smooth Muscle Cells Calcification
    Authors: E Zuccolo, I Badi, F Scavello, I Gambuzza, L Mancinelli, F Macrì, CC Tedesco, F Veglia, AR Bonfigli, F Olivieri, A Raucci
    Int J Mol Sci, 2020-06-23;21(12):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Identification of hub genes and key pathways of dietary advanced glycation end products?induced non?alcoholic fatty liver disease by bioinformatics analysis and animal experiments
    Authors: J Wang, H Liu, G Xie, W Cai, J Xu
    Mol Med Rep, 2019-12-09;21(2):685-694.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Glucose Ingestion Inhibits Endurance Exercise-Induced IL-6 Producing Macrophage Infiltration in Mice Muscle
    Authors: T Tominaga, S Ma, K Saitou, K Suzuki
    Nutrients, 2019-06-30;11(7):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. MicroRNA-29b Enhances Osteoclast Survival by Targeting BCL-2-Modifying Factor after Lipopolysaccharide Stimulation
    Authors: OJ Sul, M Rajasekara, HJ Park, JH Suh, HS Choi
    Oxid Med Cell Longev, 2019-04-10;2019(0):6018180.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  16. RIPK1 inhibition attenuates experimental autoimmune arthritis via suppression of osteoclastogenesis
    Authors: J Jhun, SH Lee, SY Kim, J Ryu, JY Kwon, HS Na, K Jung, SJ Moon, ML Cho, JK Min
    J Transl Med, 2019-03-15;17(1):84.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. Clemastine Alleviates Depressive-Like Behavior Through Reversing the Imbalance of Microglia-Related Pro-inflammatory State in Mouse Hippocampus
    Authors: WJ Su, T Zhang, CL Jiang, W Wang
    Front Cell Neurosci, 2018-11-13;12(0):412.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  18. Evidence for acute contraction-induced myokine secretion by C2C12 myotubes
    Authors: Y Furuichi, Y Manabe, M Takagi, M Aoki, NL Fujii
    PLoS ONE, 2018-10-24;13(10):e0206146.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  19. BATF regulates collagen-induced arthritis by regulating T helper cell differentiation
    Authors: SH Park, J Rhee, SK Kim, JA Kang, JS Kwak, YO Son, WS Choi, SG Park, JS Chun
    Arthritis Res. Ther., 2018-08-02;20(1):161.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. 3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress via ERK1/2 Phosphorylation
    Authors: LL Xu, T Liu, L Wang, L Li, YF Wu, CC Li, B Di, QD You, ZY Jiang
    Oxid Med Cell Longev, 2018-05-20;2018(0):3271617.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Inhibiting the NLRP3 inflammasome with MCC950 promotes non-phlogistic clearance of amyloid-? and cognitive function in APP/PS1 mice
    Authors: C Dempsey
    Brain Behav. Immun, 2016-12-18;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  22. CD8+ T cells produce a dialyzable antigen-specific activator of dendritic cells
    J Leukoc Biol, 2016-08-11;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  23. Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism
    Authors: C Zhao, W Zhang, Y Zhao, Y Yang, H Luo, G Ji, E Dong, H Deng, S Lin, Y Wei, H Yang
    Sci Rep, 2016-01-14;6(0):19404.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Lipocalin 2 is a regulator of macrophage polarization and NF-kappaB/STAT3 pathway activation.
    Authors: Guo H, Jin D, Chen X
    Mol Endocrinol, 2014-08-15;28(10):1616-28.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Respiratory tract epithelial cells express retinaldehyde dehydrogenase ALDH1A and enhance IgA production by stimulated B cells in the presence of vitamin A.
    Authors: Rudraraju R, Jones B, Surman S, Sealy R, Thomas P, Hurwitz J
    PLoS ONE, 2014-01-22;9(1):e86554.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  26. Dysregulation of suppressor of cytokine signaling 3 in keratinocytes causes skin inflammation mediated by interleukin-20 receptor-related cytokines.
    Authors: Uto-Konomi A, Miyauchi K, Ozaki N
    PLoS ONE, 2012-07-05;7(7):e40343.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  27. IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells.
    Authors: Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS
    J. Clin. Invest., 2011-09-19;121(10):3846-59.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  28. Tumor development in murine ulcerative colitis depends on MyD88 signaling of colonic F4/80+CD11b(high)Gr1(low) macrophages.
    Authors: Schiechl G, Bauer B, Fuss I, Lang SA, Moser C, Ruemmele P, Rose-John S, Neurath MF, Geissler EK, Schlitt HJ, Strober W, Fichtner-Feigl S
    J. Clin. Invest., 2011-04-25;121(5):1692-708.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  29. Uropathogenic Escherichia coli-induced inflammation alters mouse urinary bladder contraction via an interleukin-6-activated inducible nitric oxide synthase-related pathway.
    Authors: Weng TI, Wu HY, Lin PY, Liu SH
    Infect. Immun., 2009-05-26;77(8):3312-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  30. Foxa1 and Foxa2 regulate bile duct development in mice.
    Authors: Li Z, White P, Tuteja G, Rubins N, Sackett S, Kaestner KH
    J. Clin. Invest., 2009-05-11;119(6):1537-45.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC-Fr, Neutralization
  31. The critical role of invading peripheral macrophage-derived interleukin-6 in vincristine-induced mechanical allodynia in mice.
    Authors: Kiguchi N, Maeda T, Kobayashi Y, Kondo T, Ozaki M, Kishioka S
    Eur. J. Pharmacol., 2008-07-10;592(1):87-92.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  32. Particulate matter exposure induces persistent lung inflammation and endothelial dysfunction.
    Authors: Tamagawa E, Bai N, Morimoto K, Gray C, Mui T, Yatera K, Zhang X, Xing L, Li Y, Laher I, Sin DD, Man SF, van Eeden SF
    Am. J. Physiol. Lung Cell Mol. Physiol., 2008-05-09;295(1):L79-85.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC
  33. Neuritogenic effects of T cell-derived IL-3 on mouse splenic sympathetic neurons in vivo.
    Authors: Kannan-Hayashi Y, Okamura K, Hattori S, Kuwamura M, Higuchi E, Terayama H, Moriyama M, Mukamoto M, Okada M, Ohsugi Y, Nakamura Y
    J. Immunol., 2008-03-15;180(6):4227-34.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  34. Mechanisms of interleukin-6 synthesis and release induced by interleukin-1 and cell depolarisation in neurones.
    Authors: Tsakiri N, Kimber I, Rothwell NJ, Pinteaux E
    Mol. Cell. Neurosci., 2007-09-11;37(1):110-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  35. Interleukin-6 as a mechanism for the adverse effects of social stress on acute Theiler's virus infection.
    Authors: Meagher MW, Johnson RR, Young EE, Vichaya EG, Lunt S, Hardin EA, Connor MA, Welsh CJ
    Brain Behav. Immun., 2007-06-25;21(8):1083-95.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  36. High pressure promotes monocyte adhesion to the vascular wall.
    Authors: Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S
    Circ. Res., 2007-03-29;100(8):1226-33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  37. Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells.
    Authors: Kurooka M, Kaneda Y
    Cancer Res., 2007-01-01;67(1):227-36.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  38. The sex steroid precursor DHEA accelerates cutaneous wound healing via the estrogen receptors.
    Authors: Mills SJ, Ashworth JJ, Gilliver SC, Hardman MJ, Ashcroft GS
    J. Invest. Dermatol., 2005-11-01;125(5):1053-62.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  39. The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
    Authors: Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
    Arch. Dermatol. Res., 2001-11-01;293(10):508-14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  40. A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
    Authors: Shih CC, Hu MC, Hu J
    Blood, 2000-03-15;95(6):1957-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  41. CCN2 participates in overload-induced skeletal muscle hypertrophy
    Authors: Jennifer M. Petrosino, Jacob Z. Longenecker, Colin D. Angell, Scott A. Hinger, Colton R. Martens, Federica Accornero
    Matrix Biology
  42. Efferocytosis induces macrophage proliferation to help resolve tissue injury
    Authors: Gerlach BD, Ampomah PB, Yurdagul A Et al.
    Cell metabolism
  43. Neuroectodermal Stem Cells Grafted into the Injured Spinal Cord Induce Both Axonal Regeneration and Morphological Restoration via Multiple Mechanisms
    Authors: Krisztián Pajer, Tamás Bellák, Heinz Redl, Antal Nógrádi
    Journal of Neurotrauma
  44. Cytokine signaling by grafted neuroectodermal stem cells rescues motoneurons destined to die
    Authors: Krisztián Pajer, Georg A. Feichtinger, Gábor Márton, Sonja Sabitzer, Dieter Klein, Heinz Redl et al.
    Experimental Neurology
  45. Social disruption induced priming of CNS inflammatory response to Theiler's virus is dependent upon stress induced IL-6 release
    Authors: E.G. Vichaya, E.E. Young, M.A. Frazier, J.L. Cook, C.J. Welsh, M.W. Meagher
    Journal of Neuroimmunology
  46. The inflammatory signalling mediator TAK1 mediates lymphocyte recruitment to lipopolysaccharide-activated murine mesenchymal stem cells through interleukin-6
    Authors: Beatrice Oelze, Kirsten Elger, Patrik Schadzek, Laura Burmeister, Anika Hamm, Sandra Laggies et al.
    Molecular and Cellular Biochemistry
  47. Vitronectin mitigates stroke-increased neurogenesis only in female mice and through FAK-regulated IL-6
    Authors: Cuihong Jia, Matthew P. Keasey, Hannah M. Malone, Chiharu Lovins, Theo Hagg
    Experimental Neurology
  48. IL-6 Trans-Signaling Drives Murine Crescentic GN.
    Authors: Braun GS, Nagayama Y, Maruta Y et al.
    J. Am. Soc. Nephrol.

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Reviews for Mouse IL-6 Antibody

Average Rating: 3.8 (Based on 6 Reviews)

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Mouse IL-6 Antibody
By Anonymous on 02/14/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: A20 mouse B cell lymphoma cell line Species: Mouse
Immunocytochemistry/Immunofluorescence Mouse IL-6 Antibody AF-406-NA
Mouse IL-6 Antibody
By Anonymous on 07/06/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Connective tissue Species: Rat
Immunocytochemistry/Immunofluorescence Mouse IL-6 Antibody AF-406-NA
Mouse IL-6 Antibody
By Anonymous on 04/06/2021
Application: WB Sample Tested: Adipose tissue Species: Human and Human Endothelial cell

Protein from adipose tissue that was isolated with WT and KO mice, were subjected to immunoblotting for IL-6.

Western Blot Mouse IL-6 Antibody AF-406-NA
Mouse IL-6 Antibody
By Anonymous on 12/18/2020
Application: ELISA Sample Tested: Serum Species: Mouse
ELISA Mouse IL-6 Antibody AF-406-NA
Mouse IL-6 Antibody
By Tina Yuan on 11/23/2020
Application: IHC Sample Tested: mouse bone tissue Species: Mouse
Immunohistochemistry Mouse IL-6 Antibody AF-406-NA
Mouse IL-6 Antibody
By Anonymous on 10/13/2020
Application: WB Sample Tested: Pancreas tissue Species: Mouse

***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Western Blot Mouse IL-6 Antibody AF-406-NA