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Mouse p53 Antibody

Catalog #: MAB1746
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1746
MAB1746-SP
Immunoprecipitation of Mouse p53.
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Product Details
Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse p53 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse p53 in Western blots.
Source
Monoclonal Rat IgG2B Clone # 197643
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse p53
Leu28-Phe325
Accession # NP_035770
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunoprecipitation
1-3 µg/500 µg cell lysate
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunoprecipitation Immunoprecipitation of Mouse p53. View Larger

Immunoprecipitation of Mouse p53. Balb/3T3 mouse embryonic fibroblast cell line was untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 6 hours. p53 was immunoprecipitated from 500 µg of cell lysate following incubation with 1-3 µg Rat Anti-Mouse p53 Monoclonal Antibody (Catalog # MAB1746) for 1 hour on ice. p53-antibody complexes were absorbed using goat anti-rat IgG sepharose (Invitrogen). Immunoprecipitated p53 was detected by Western blot using a 1:5000 dilution of Human/Mouse/Rat p53 Horseradish Peroxidase-conjugated Antigen Affinity-purified Polyclonal Antibody (HAF1355). View our recommended buffer recipes for immunoprecipitation.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p53

The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.

Entrez Gene IDs
7157 (Human); 22059 (Mouse); 24842 (Rat)
Alternate Names
Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53

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Citations for Mouse p53 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Combined HDAC1 and HDAC2 Depletion Promotes Retinal Ganglion Cell Survival After Injury Through Reduction of p53 Target Gene Expression
    Authors: Frédéric Lebrun-Julien, Ueli Suter
    ASN Neuro
  2. Cancer cells same as zombies reprogram normal cells via the secreted microenvironment
    Authors: Rabiee, S;Hoveizi, E;Barati, M;Salehzadeh, A;Joghataei, MT;Tavakol, S;
    PloS one
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Early-life nutrition influences thymic growth in male mice that may be related to the regulation of longevity.
    Authors: Chen JH, Tarry-Adkins JL, Heppolette CA, Palmer DB, Ozanne SE
    Clin. Sci., 2009-12-14;118(6):429-38.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Adverse effects of reduced oxygen tension on the proliferative capacity of rat kidney and insulin-secreting cell lines involve DNA damage and stress responses.
    Authors: Chen JH, Jones RH, Tarry-Adkins J, Smith NH, Ozanne SE
    Exp. Cell Res., 2008-07-28;314(16):3075-80.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

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