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Mouse Sca-1/Ly6 Antibody

Catalog #: AF1226
27 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1226
AF1226-SP
Sca‑1/Ly6 in Mouse Splenocytes.
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Product Details
Citations (27)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Sca-1/Ly6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Sca-1/Ly6 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Sca-1 C-terminally truncated Ly-6E allele
Leu27-Gly119
Accession # CAA28351
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Sca‑1/Ly6
Flow Cytometry
2.5 µg/106 cells
Mouse splenocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Sca‑1/Ly6 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

Sca‑1/Ly6 in Mouse Splenocytes. Sca-1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse Sca-1/Ly6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Western Blot Detection of Mouse Sca-1/Ly6 by Western Blot View Larger

Detection of Mouse Sca-1/Ly6 by Western Blot Sca1, a marker of mammary stem/progenitor cells, is increased in DNIIR and Wnt5a-/- glands. mRNA and protein were extracted from several wild type (WT) and MT-DNIIR (DNIIR) mice. (a) Sca1 mRNA levels were determined by semi-quantitative RT-PCR and (b) protein levels were determined by western blot. Sca1 levels were increased in DNIIR glands relative to controls. (c) Sca1 protein levels were determined in WT and Wnt5a null (-/-) glands using western blot analysis. Sca1 levels were increased in Wnt5a-null tissue relative to controls. 18S and beta -Tubulin ( beta -Tub) were used as normalisation controls. DNIIR = dominant-negative transforming growth factor-beta type II receptor; RT-PCR = reverse transcripase polymerase chain reaction; Wnt = Wingless-related protein family. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/19344510), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Sca-1/Ly6 by Western Blot View Larger

Detection of Mouse Sca-1/Ly6 by Western Blot Sca1, a marker of mammary stem/progenitor cells, is increased in DNIIR and Wnt5a-/- glands. mRNA and protein were extracted from several wild type (WT) and MT-DNIIR (DNIIR) mice. (a) Sca1 mRNA levels were determined by semi-quantitative RT-PCR and (b) protein levels were determined by western blot. Sca1 levels were increased in DNIIR glands relative to controls. (c) Sca1 protein levels were determined in WT and Wnt5a null (-/-) glands using western blot analysis. Sca1 levels were increased in Wnt5a-null tissue relative to controls. 18S and beta -Tubulin ( beta -Tub) were used as normalisation controls. DNIIR = dominant-negative transforming growth factor-beta type II receptor; RT-PCR = reverse transcripase polymerase chain reaction; Wnt = Wingless-related protein family. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/19344510), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Sca-1/Ly6 by Western Blot View Larger

Detection of Mouse Sca-1/Ly6 by Western Blot Ectopic expression of Wnt5a results in low expression of K6 and K14.Expression of molecular markers for basal and luminal progenitors in MMTV-Wnt1 and MMTV-Wnt1;MMTV-Wnt5a mammary glands was compared by western blot. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. Each lane contains protein isolated from a separate mouse. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0113247), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Sca-1/Ly6 by Western Blot View Larger

Detection of Mouse Sca-1/Ly6 by Western Blot Sca1, a marker of mammary stem/progenitor cells, is increased in DNIIR and Wnt5a-/- glands. mRNA and protein were extracted from several wild type (WT) and MT-DNIIR (DNIIR) mice. (a) Sca1 mRNA levels were determined by semi-quantitative RT-PCR and (b) protein levels were determined by western blot. Sca1 levels were increased in DNIIR glands relative to controls. (c) Sca1 protein levels were determined in WT and Wnt5a null (-/-) glands using western blot analysis. Sca1 levels were increased in Wnt5a-null tissue relative to controls. 18S and beta -Tubulin ( beta -Tub) were used as normalisation controls. DNIIR = dominant-negative transforming growth factor-beta type II receptor; RT-PCR = reverse transcripase polymerase chain reaction; Wnt = Wingless-related protein family. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/19344510), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Sca-1/Ly6 by Western Blot View Larger

Detection of Mouse Sca-1/Ly6 by Western Blot Wnt5a expressing tumors demonstrate a decrease in markers of the basal tumor subtype.(A) Western blot using protein lysates isolated from the epithelium of MMTV-Wnt1 and MMTV-1;MMTV-Wnt5a mammary tumors. The expression of molecular markers of basal and luminal tumor subtypes were compared. Keratin 6 and Keratin 5 were strongly down-regulated in Wnt5a expressing tumors. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. (B–D) Immunostaining for K6. Sections from MMTV-Wnt1 (B) and MMTV-1;MMTV-Wnt5a (C) tumors were stained with anti-Keratin 6 antibody using immunofluorescence (K6 = green; nuclei = blue). The percentage of cells expressing K6 was determined and graphed (D). Values are means +/− standard error (n = 6 MMTV-Wnt1, 3 fields per tumor; n = 5 MMTV-Wnt1;MMTV-Wnt5a, 3 fields per tumor). MMTV-Wnt1;MMTV-Wnt5a tumors demonstrated a significant decrease in K6-expressing cells as measured by T-test (* = p<0.05). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0113247), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Sca-1/Ly6 by Western Blot View Larger

Detection of Mouse Sca-1/Ly6 by Western Blot Sca1, a marker of mammary stem/progenitor cells, is increased in DNIIR and Wnt5a-/- glands. mRNA and protein were extracted from several wild type (WT) and MT-DNIIR (DNIIR) mice. (a) Sca1 mRNA levels were determined by semi-quantitative RT-PCR and (b) protein levels were determined by western blot. Sca1 levels were increased in DNIIR glands relative to controls. (c) Sca1 protein levels were determined in WT and Wnt5a null (-/-) glands using western blot analysis. Sca1 levels were increased in Wnt5a-null tissue relative to controls. 18S and beta -Tubulin ( beta -Tub) were used as normalisation controls. DNIIR = dominant-negative transforming growth factor-beta type II receptor; RT-PCR = reverse transcripase polymerase chain reaction; Wnt = Wingless-related protein family. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/19344510), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sca-1/Ly6

This antibody reacts with Sca-1, an 18 kDa phosphatidylinositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly-6) family (1). Sca-1 is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly‑6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non‑hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).

References
  1. Van de Rijn, M. et al. (1989) Proc. Natl.Acad. Sci. USA 86:4634.
  2. Spangrude, G.I. et al. (1988) Science 241:58.
  3. Spangrude, G.I. et al. (1993) Blood 82:3327.
  4. Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
  5. Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
  6. Yamamoto, Y. et al. (1996) Blood 88:445.
  7. Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
  8. Welm, B.E. et al. (2002) Dev. Biol. 245:42.
  9. Codias, E.K. et al. (1990) J. Immunol. 144:2197.
  10. Malek, T.R. et al. (1986) J. Exp. Med. 164:709 .
  11. Codias, E.K. et al. (1990) J. Immunol. 145:1407.
  12. Flood, P.M. et al. (1990) J. Exp. Med. 172:115.
Long Name
Stem Cell Antigen-1/Lymphocyte Antigen 6
Entrez Gene IDs
110454 (Mouse)
Alternate Names
Ly6; Ly-6; Ly-6A.2; Ly-6A/E; Ly-6E.1; lymphocyte antigen 6 complex, locus A; lymphocyte antigen 6A-2/6E-1; Sca1; Sca-1; Stem cell antigen 1; TAP; T-cell-activating protein

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Citations for Mouse Sca-1/Ly6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Smart Hydrogels for the Augmentation of Bone Regeneration by Endogenous Mesenchymal Progenitor Cell Recruitment
    Authors: Philipp S. Lienemann, Queralt Vallmajo‐Martin, Panagiota Papageorgiou, Ulrich Blache, Stéphanie Metzger, Anna‐Sofia Kiveliö et al.
    Advanced Science
  2. Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair
    Authors: EC Jeffery, TLA Mann, JA Pool, Z Zhao, SJ Morrison
    Cell Stem Cell, 2022-10-21;29(11):1547-1561.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. CXCL12/CXCR4-Rac1-mediated migration of osteogenic precursor cells contributes to pathological new bone formation in ankylosing spondylitis
    Authors: H Cui, Z Li, S Chen, X Li, D Chen, J Wang, Z Li, W Hao, F Zhong, K Zhang, Z Zheng, Z Zhan, H Liu
    Science Advances, 2022-04-06;8(14):eabl8054.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Bone marrow-independent adventitial macrophage progenitor cells contribute to angiogenesis
    Authors: F Kleefeldt, B Upcin, H Bömmel, C Schulz, G Eckner, J Allmanritt, J Bauer, B Braunger, U Rueckschlo, S Ergün
    Cell Death & Disease, 2022-03-09;13(3):220.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  5. Fecal microbiota transplantation ameliorates atherosclerosis in mice with C1q/TNF-related protein 9 genetic deficiency
    Authors: ES Kim, BH Yoon, SM Lee, M Choi, EH Kim, BW Lee, SY Kim, CG Pack, YH Sung, IJ Baek, CH Jung, TB Kim, JY Jeong, CH Ha
    Experimental & Molecular Medicine, 2022-02-03;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Distinct Expression Patterns of Cxcl12 in Mesenchymal Stem Cell Niches of Intact and Injured Rodent Teeth
    Authors: P Pagella, C Nombela-Ar, TA Mitsiadis
    International Journal of Molecular Sciences, 2021-03-16;22(6):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2017-11-13;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms
    Authors: L Golle, HU Gerth, K Beul, B Heitplatz, P Barth, M Fobker, H Pavenstädt, GS Di Marco, M Brand
    Sci Rep, 2017-08-25;7(1):9444.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Dipeptidyl Peptidase-4 Regulates Hematopoietic Stem Cell Activation in Response to Chronic Stress
    Authors: E Zhu, L Hu, H Wu, L Piao, G Zhao, A Inoue, W Kim, C Yu, W Xu, YK Bando, X Li, Y Lei, CN Hao, K Takeshita, WS Kim, K Okumura, T Murohara, M Kuzuya, XW Cheng
    J Am Heart Assoc, 2017-07-14;6(7):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  10. Systemic application of sirolimus prevents neointima formation not via a direct anti-proliferative effect but via its anti-inflammatory properties
    Authors: JM Daniel, J Dutzmann, H Brunsch, J Bauersachs, R Braun-Dull, DG Sedding
    Int. J. Cardiol., 2017-03-14;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Pluripotent stem cells derived from mouse and human white mature adipocytes.
    Authors: Jumabay M, Abdmaulen R, Ly A, Cubberly M, Shahmirian L, Heydarkhan-Hagvall S, Dumesic D, Yao Y, Bostrom K
    Stem Cells Transl Med, 2014-01-06;3(2):161-71.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  12. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013-12-12;504(7479):277-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  13. Age dependence of lung mesenchymal stromal cell dynamics following pneumonectomy.
    Authors: Paxson J, Gruntman A, Davis A, Parkin C, Ingenito E, Hoffman A
    Stem Cells Dev, 2013-08-30;22(24):3214-25.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Structural basis of digoxin that antagonizes RORgamma t receptor activity and suppresses Th17 cell differentiation and interleukin (IL)-17 production.
    Authors: Fujita-Sato S, Ito S, Isobe T, Ohyama T, Wakabayashi K, Morishita K, Ando O, Isono F
    J. Biol. Chem., 2011-07-06;286(36):31409-17.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Stromal cell-derived factor-1 enhances distraction osteogenesis-mediated skeletal tissue regeneration through the recruitment of endothelial precursors.
    Authors: Fujio M, Yamamoto A, Ando Y, Shohara R, Kinoshita K, Kaneko T, Hibi H, Ueda M
    Bone, 2011-06-29;49(4):693-700.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  16. Three-dimensional culture of mouse renal carcinoma cells in agarose macrobeads selects for a subpopulation of cells with cancer stem cell or cancer progenitor properties.
    Authors: Smith BH, Gazda LS, Conn BL
    Cancer Res., 2011-01-24;71(3):716-24.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Prominin-1/CD133+ lung epithelial progenitors protect from bleomycin-induced pulmonary fibrosis.
    Authors: Germano D, Blyszczuk P, Valaperti A, Kania G, Dirnhofer S, Landmesser U, Luscher TF, Hunziker L, Zulewski H, Eriksson U
    Am. J. Respir. Crit. Care Med., 2009-02-20;179(10):939-49.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. A Gata6-Wnt pathway required for epithelial stem cell development and airway regeneration.
    Authors: Zhang Y, Goss AM, Cohen ED, Kadzik R, Lepore JJ, Muthukumaraswamy K, Yang J, DeMayo FJ, Whitsett JA, Parmacek MS, Morrisey EE
    Nat. Genet., 2008-06-08;40(7):862-70.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  19. Intestinal epithelial cell up-regulation of LY6 molecules during colitis results in enhanced chemokine secretion.
    Authors: Flanagan K, Modrusan Z, Cornelius J, Chavali A, Kasman I, Komuves L, Mo L, Diehl L
    J. Immunol., 2008-03-15;180(6):3874-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  20. The recruitment of primitive Lin(-) Sca-1(+), CD34(+), c-kit(+) and CD271(+) cells during the early intraperitoneal foreign body reaction.
    Authors: Vranken I, De Visscher G, Lebacq A, Verbeken E, Flameng W
    Biomaterials, 2007-11-26;29(7):797-808.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  21. Identification of pulmonary Oct-4+ stem/progenitor cells and demonstration of their susceptibility to SARS coronavirus (SARS-CoV) infection in vitro.
    Authors: Ling TY, Kuo MD, Li CL, Yu AL, Huang YH, Wu TJ, Lin YC, Chen SH, Yu J
    Proc. Natl. Acad. Sci. U.S.A., 2006-06-13;103(25):9530-5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  22. Identification of bronchioalveolar stem cells in normal lung and lung cancer.
    Authors: Kim CF, Jackson EL, Woolfenden AE, Lawrence S, Babar I, Vogel S, Crowley D, Bronson RT, Jacks T
    Cell, 2005-06-17;121(6):823-35.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  23. Epidermal beta -catenin activation remodels the dermis via paracrine signalling to distinct fibroblast lineages
    Authors: Beate M. Lichtenberger, Maria Mastrogiannaki, Fiona M. Watt
    Nature Communications
  24. Genetic lineage tracing of targeted cell populations during enthesis healing
    Authors: Helen L. Moser, Anton Ph. Doe, Kristen Meier, Simon Garnier, Damien Laudier, Haruhiko Akiyama et al.
    Journal of Orthopaedic Research®
  25. Wnt5a Suppresses Tumor Formation and Redirects Tumor Phenotype in MMTV-Wnt1 Tumors
    Authors: Stephanie L. Easter, Elizabeth H. Mitchell, Sarah E. Baxley, Renee Desmond, Andra R. Frost, Rosa Serra
    PLoS ONE
  26. Tgfbeta signaling is critical for maintenance of the tendon cell fate
    Authors: Tan GK, Pryce BA, Stabio A et al.
    Elife
  27. Bone marrow-derived cells rescue salivary gland function in mice with head and neck irradiation
    Authors: Yoshinori Sumita, Younan Liu, Saeed Khalili, Ola M. Maria, Dengsheng Xia, Sharon Key et al.
    The International Journal of Biochemistry & Cell Biology

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Mouse Sca-1/Ly6 Antibody
By Adam Guess on 11/08/2016
Application: Flow Sample Tested: bone marrow Species: Mouse
Flow Cytometry Mouse Sca-1/Ly6 Antibody AF1226