Mouse Siglec-G Antibody

Catalog #: MAB7775 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7775
MAB7775-SP

All Siglec-G Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Siglec-G Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Siglec-G in ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Siglec-F or recombinant human Siglec-10 is observed.

Source

Monoclonal Rat IgG_2A Clone # 805903

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Siglec-G
Glu20-Gln525
Accession # NP_766488

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

Mouse CD11b⁺ splenocytes

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-G

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by a V-type Ig-like domain which mediates sialic acid binding, followed by varying numbers of C2-type Ig-like domains. Mouse Siglec-G, the apparent ortholog of human Siglec-10, is a 110-120 kDa, 688 amino acid (aa) type I transmembrane protein mainly expressed on mouse B1-type B cells. It controls B1 cell survival, selection, expansion and calcium signaling by negatively regulating B cell receptor signals. A potentially secreted 269 aa variant diverges after the first two Ig-like domains. Mouse Siglec-G shares approximately 63% aa sequence identity with human Siglec-10 within the extracellular domain.

Long Name

Sialic Acid Binding Ig-like Lectin G

Entrez Gene IDs

243958 (Mouse)

Alternate Names

PRO940;Sialic acid-binding Ig-like lectin 10;SIGLEC10;SIGLEC-10;SLG2; Siglec-10; SiglecG; Siglec-G

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse Siglec-G Antibody

There are currently no reviews for this product. Be the first to review Mouse Siglec-G Antibody and earn rewards!

Have you used Mouse Siglec-G Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review