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Proteome Profiler Mouse Cytokine Array Kit, Panel A

Catalog #: ARY006
406 Citations 36 Reviews Datasheet / COA / SDS
Contains 4 membranes - each spotted in duplicate with 40 different cytokine antibodies
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ARY006
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The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample.
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Proteome Profiler Mouse Cytokine Array Kit, Panel A Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected mouse cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and plasma.

Troubleshooting Guide

Key Benefits

  • Detects 40 mouse cytokines, chemokines, and acute phase proteins simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR* and chemiluminescence detection
*If using a LI-COR, additional reagents and protocol modifications are required.  Refer to protocol.

Principle of the Assay

The Proteome Profiler Mouse Cytokine Array Kit, Panel A is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

 

 

Kit Contents
  • Rectangular 4-Well Multi-dish
  • 4 Mouse Cytokine Array, Panel A nitrocellulose membranes spotted with 40 different antibodies to mouse cytokines, chemokines, and acute phase proteins
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Mouse Cytokine Array, Panel A
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.  

 
Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
CXCL13/BLC/BCA-1 IL-5 M-CSF
C5a IL-6 CCL2/JE/MCP-1
G-CSF IL-7 CCL12/MCP-5
GM-CSF IL-10 CXCL9/MIG
CCL1/I-309 IL-12 p70 CCL3/MIP-1 alpha
CCL11/Eotaxin IL-13 CCL4/MIP-1 beta
ICAM-1 IL-16 CXCL2/MIP-2
IFN-gamma IL-17 CCL5/RANTES
IL-1 alpha/IL-1F1 IL-23 CXCL12/SDF-1
IL-1 beta/IL-1F2 IL-27 CCL17/TARC
IL-1ra/IL-1F3 CXCL10/IP-10 TIMP-1
IL-2 CXCL11/I-TAC TNF-alpha
IL-3 CXCL1/KC TREM-1
IL-4    

 

Assays for Analytes represented in the Mouse Cytokine Array Kit, Panel A

Analyte Quantikine® ELISA Kits DuoSet® ELISA Development Systems
CXCL13/BLC/BCA-1 MCX130 DY470
C5a   DY2150
G-CSF MCS00 DY414
GM-CSF MGM00 DY415
CCL1/I-309/TCA-3   DY845
CCL11/Eotaxin MME00 DY420
ICAM-1 MIC100 DY796
IFN-gamma MIF00 DY485
IL-1 alpha MLA00 DY400
IL-1 beta MLB00B DY401
IL-1ra MRA00 DY480
IL-2 M2000 DY402
IL-3 M3000 DY403
IL-4 M4000B DY404
IL-5 M5000 DY405
IL-6 M6000B DY406
IL-7 M7000 DY407
IL-10 M1000b DY417
IL-12 p70 M1270 DY419
IL-13 M1300CB DY413
IL-16    
IL-17 M1700 DY421
IL-23 M2300 DY1887
IL-27 M2728  
CXCL10/IP-10/CRG-2 MCX100 DY466
CXCL11/I-TAC   DY572
CXCL1/KC MKC00B DY453
M-CSF MMC00 DY416
CCL2/MCP-1/JE MJE00 DY479
CCL12/MCP-5 MCC120 DY428
CXCL9/MIG MCX900 DY492
CCL3/MIP-1 alpha MMA00 DY450
CCL4/MIP-1 beta MMB00 DY451
CXCL2/MIP-2 MM200 DY452
CCL5/RANTES MMR00 DY478
CXCL12/SDF-1 MCX120 DY460
CCL17/TARC   DY529
TIMP-1 MTM100 DY980
TNF-alpha MTA00 DY410
TREM-1 MTRM10 DY1187

Specifications

Source
N/A
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Mouse

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Scientific Data

N/A The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample. View Larger

The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample. Mouse splenocytes treated with 100 ng/mL LPS for 20 hours were mixed with a cocktail of biotinylated detection antibodies, and then incubated with the Mouse Cytokine Array. The array was then incubated with streptavidin-horseradish peroxidase followed by chemiluminescent detection. After detection, the array data were quantitated to generate a protein profile (histogram). The table shows the analytes detected and their location on the membrane.

N/A The Mouse Cytokine Array detects multiple analytes in cell culture lysates and supernates. View Larger

The Mouse Cytokine Array detects multiple analytes in cell culture lysates and supernates. (A. and B.) Lysates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µg of lysate was used for each array shown. (C. and D.) Supernates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µL of supernate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

N/A The Mouse Cytokine Array detects multiple analytes in mouse serum and tissue lysates. View Larger

The Mouse Cytokine Array detects multiple analytes in mouse serum and tissue lysates. (A and B.) Samples of mouse serum from two different vendors. 200 µL of serum was used for each array shown. (C. and D.) Lysates of mouse lung tissue and mouse stomach tissue. 200 µg of lysate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

Citations for Proteome Profiler Mouse Cytokine Array Kit, Panel A

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

406 Citations: Showing 1 - 10
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  1. Immunocompatible elastomer with increased resistance to the foreign body response
    Authors: Zhou, X;Lu, Z;Cao, W;Zhu, Z;Chen, Y;Ni, Y;Liu, Z;Jia, F;Ye, Y;Han, H;Yao, K;Liu, W;Wang, Y;Ji, J;Zhang, P;
    Nature communications  2024-08-30
  2. RNA shielding of P65 is required to potentiate oncogenic inflammation in TET2 mutated clonal hematopoiesis
    Authors: Ben-Crentsil, NA;Mohammed Ismail, W;Balasis, ME;Newman, H;Quintana, A;Binder, M;Kruer, T;Neupane, S;Ferrall-Fairbanks, MC;Fernandez, J;Lasho, TL;Finke, CM;Ibrahim, ML;McGraw, KL;Wysota, M;Aldrich, AL;Ryder, CB;Letson, CT;Traina, J;McLemore, AF;Droin, N;Shastri, A;Yun, S;Solary, E;Sallman, DA;Beg, AA;Ma, L;Gaspar-Maia, A;Patnaik, MM;Padron, E;
    Cancer discovery  2024-08-27
  3. The intersection of endocrine signaling and neuroimmune communication regulates neonatal nociception
    Authors: Fadaka, AO;Dourson, AJ;Hofmann, MC;Gupta, P;Raut, NGR;Jankowski, MP;
    bioRxiv : the preprint server for biology  2024-08-07
  4. Adenosine triggers early astrocyte reactivity that provokes microglial responses and drives the pathogenesis of sepsis-associated encephalopathy in mice
    Authors: Guo, Q;Gobbo, D;Zhao, N;Zhang, H;Awuku, NO;Liu, Q;Fang, LP;Gampfer, TM;Meyer, MR;Zhao, R;Bai, X;Bian, S;Scheller, A;Kirchhoff, F;Huang, W;
    Nature communications  2024-07-27
  5. Targeting conserved TIM3+VISTA+ tumor-associated macrophages overcomes resistance to cancer immunotherapy
    Authors: Vanmeerbeek, I;Naulaerts, S;Sprooten, J;Laureano, RS;Govaerts, J;Trotta, R;Pretto, S;Zhao, S;Cafarello, ST;Verelst, J;Jacquemyn, M;Pociupany, M;Boon, L;Schlenner, SM;Tejpar, S;Daelemans, D;Mazzone, M;Garg, AD;
    Science advances  2024-07-19
  6. Sphinganine recruits TLR4 adaptors in macrophages and promotes inflammation in murine models of sepsis and melanoma
    Authors: Hering, M;Madi, A;Sandhoff, R;Ma, S;Wu, J;Mieg, A;Richter, K;Mohr, K;Knabe, N;Stichling, D;Poschet, G;Bestvater, F;Frank, L;Utikal, J;Umansky, V;Cui, G;
    Nature communications  2024-07-18
  7. Tumor cell-intrinsic MELK enhanced CCL2-dependent immunosuppression to exacerbate hepatocarcinogenesis and confer resistance of HCC to radiotherapy
    Authors: Tang, B;Zhu, J;Shi, Y;Wang, Y;Zhang, X;Chen, B;Fang, S;Yang, Y;Zheng, L;Qiu, R;Weng, Q;Xu, M;Zhao, Z;Tu, J;Chen, M;Ji, J;
    Molecular cancer  2024-07-05
  8. Cardiotrophin-1 therapy reduces disease severity in a murine model of glomerular disease
    Authors: Perretta-Tejedor, N;Price, KL;Jafree, DJ;Pomeranz, G;Kolatsi-Joannou, M;Martínez-Salgado, C;Long, DA;Vasilopoulou, E;
    Physiological reports  2024-07-01
  9. Cell cycle-dependent centrosome clustering precedes proplatelet formation
    Authors: Becker, IC;Wilkie, AR;Nikols, E;Carminita, E;Roweth, HG;Tilburg, J;Sciaudone, AR;Noetzli, LJ;Fatima, F;Couldwell, G;Ray, A;Mogilner, A;Machlus, KR;Italiano, JE;
    Science advances  2024-06-21
  10. Inhibition of EGFR/ErbB does not protect against C. difficile toxin B
    Authors: Siddiqi, U;Lunnemann, HM;Childress, KO;Shupe, JA;Rutherford, SA;Farrow, MA;Washington, MK;Coffey, RJ;Lacy, DB;Markham, NO;
    bioRxiv : the preprint server for biology  2024-05-14
  11. Colon impairments and inflammation driven by an altered gut microbiota leads to social behavior deficits rescued by hyaluronic acid and celecoxib
    Authors: Agranyoni, O;Sur, D;Amidror, S;Shidlovsky, N;Bagaev, A;Yissachar, N;Pinhasov, A;Navon-Venezia, S;
    BMC medicine  2024-04-29
  12. The Effect and Mechanism of Astragalus Polysaccharides on T Cells and Macrophages in Inhibiting Prostate Cancer
    Authors: Wu, CY;Yang, YH;Lin, YS;Shu, LH;Liu, HT;Lu, CK;Wu, YH;Wu, YH;
    Biomedical journal  2024-04-25
  13. Pharmacological expansion of type 2 alveolar epithelial cells promotes regenerative lower airway repair
    Authors: Shao, S;Zhang, N;Specht, GP;You, S;Song, L;Fu, Q;Huang, D;You, H;Shu, J;Domissy, A;Li, S;Nguyen-Tran, V;Joseph, SB;Chatterjee, AK;Chen, JJ;Schultz, PG;Bollong, MJ;
    Proceedings of the National Academy of Sciences of the United States of America  2024-04-16
  14. The Hippo kinases control inflammatory Hippo signaling and restrict bacterial infection in phagocytes
    Authors: St Louis, BM;Quagliato, SM;Su, Y-T;Dyson, G;Lee, P-C;
    mBio  2024-04-16
  15. Early-life exercise induces immunometabolic epigenetic modification enhancing anti-inflammatory immunity in middle-aged male mice
    Authors: Zhang, N;Wang, X;Feng, M;Li, M;Wang, J;Yang, H;He, S;Xia, Z;Shang, L;Jiang, X;Sun, M;Wu, Y;Ren, C;Zhang, X;Li, J;Gao, F;
    Nature communications  2024-04-10
  16. Dietary emulsifier polysorbate 80 exposure accelerates age-related cognitive decline
    Authors: Zhang, L;Yin, Z;Liu, X;Jin, G;Wang, Y;He, L;Li, M;Pang, X;Yan, B;Jia, Z;Ma, J;Wei, J;Cheng, F;Li, D;Wang, L;Han, Z;Liu, Q;Chen, F;Cao, H;Lei, P;
    Brain, behavior, and immunity  2024-03-31
  17. Developing inhibitory peptides against SARS-CoV-2 envelope protein
    Authors: Bekdash, R;Yoshida, K;Nair, MS;Qiu, L;Ahdout, J;Tsai, HY;Uryu, K;Soni, RK;Huang, Y;Ho, DD;Yazawa, M;
    PLoS biology  2024-03-01
  18. Identification of IGF-1 Effects on White Adipose Tissue and Hippocampus in Alzheimer's Disease Mice via Transcriptomic and Cellular Analysis
    Authors: Kim, YK;Jo, D;Arjunan, A;Ryu, Y;Lim, YH;Choi, SY;Kim, HK;Song, J;
    International journal of molecular sciences  2024-02-22
  19. Plasticity-induced repression of Irf6 underlies acquired resistance to cancer immunotherapy in pancreatic ductal adenocarcinoma
    Authors: Kim, IK;Diamond, MS;Yuan, S;Kemp, SB;Kahn, BM;Li, Q;Lin, JH;Li, J;Norgard, RJ;Thomas, SK;Merolle, M;Katsuda, T;Tobias, JW;Baslan, T;Politi, K;Vonderheide, RH;Stanger, BZ;
    Nature communications  2024-02-20
  20. Tumor cell derived osteopontin and prostaglandin E2 synergistically promote the expansion of myeloid derived suppressor cells during the tumor immune escape phase
    Authors: Mamand, DR;Bazaz, S;Mohammad, DK;Saher, O;Wiklander, OPB;Sadeghi, B;Hassan, M;El-Andaloussi, S;Abedi-Valugerdi, M;
    International immunopharmacology  2024-02-15
  21. Neutralizing IL-16 enhances the efficacy of targeting Aurora-A therapy in colorectal cancer with high lymphocyte infiltration through restoring anti-tumor immunity
    Authors: Yang, SJ;Chang, ST;Chang, KC;Lin, BW;Chang, KY;Liu, YW;Lai, MD;Hung, LY;
    Cell death & disease  2024-01-30
  22. CHCHD4-TRIAP1 regulation of innate immune signaling mediates skeletal muscle adaptation to exercise
    Authors: Ma, J;Wang, PY;Zhuang, J;Son, AY;Karius, AK;Syed, AM;Nishi, M;Wu, Z;Mori, MP;Kim, YC;Hwang, PM;
    Cell reports  2023-12-28
  23. Heterogeneous ferroptosis susceptibility of macrophages caused by focal iron overload exacerbates rheumatoid arthritis
    Authors: Liu, Y;Luo, X;Chen, Y;Dang, J;Zeng, D;Guo, X;Weng, W;Zhao, J;Shi, X;Chen, J;Dong, B;Zhong, S;Ren, J;Li, Y;Wang, J;Zhang, J;Sun, J;Xu, H;Lu, Y;Brand, D;Zheng, SG;Pan, Y;
    Redox biology  2023-12-20
  24. Amyloid-? specific regulatory T cells attenuate Alzheimer's disease pathobiology in APP/PS1 mice
    Authors: Yeapuri, P;Machhi, J;Lu, Y;Abdelmoaty, MM;Kadry, R;Patel, M;Bhattarai, S;Lu, E;Namminga, KL;Olson, KE;Foster, EG;Mosley, RL;Gendelman, HE;
    Molecular neurodegeneration  2023-12-18
  25. Multipotent Mesenchymal Cells Homing and Differentiation on Poly(?-caprolactone) Blended with 20% Tricalcium Phosphate and Polylactic Acid Incorporating 10% Hydroxyapatite 3D-Printed Scaffolds via a Commercial Fused Deposition Modeling 3D Device
    Authors: De Angelis, N;Amaroli, A;Lagazzo, A;Barberis, F;Zarro, PR;Cappelli, A;Sabbieti, MG;Agas, D;
    Biology  2023-11-28
  26. Lymphatic Dysfunction Models an Autoimmune Emphysema Phenotype of Chronic Obstructive Pulmonary Disease
    Authors: Summers, B;Kim, K;Lu, TM;Houghton, S;Trivedi, A;Quintero, JR;Cala-Garcia, J;Pannellini, T;Polverino, F;Lis, R;Reed, HO;
    bioRxiv : the preprint server for biology  2023-11-02
  27. A dual role of lysophosphatidic acid type 2 receptor (LPAR2) in nonsteroidal anti-inflammatory drug-induced mouse enteropathy
    Authors: Hutka, B;Várallyay, A;László, SB;Tóth, AS;Scheich, B;Paku, S;Vörös, I;Pós, Z;Varga, ZV;Norman, DD;Balogh, A;Benyó, Z;Tigyi, G;Gyires, K;Zádori, ZS;
    Acta pharmacologica Sinica  2023-10-10
  28. Role Of The C-C Motif Chemokine Ligand 5 (CCL5) And Its Receptor, C-C Motif Chemokine Receptor 5 (CCR5) In The Genesis Of Aldosterone-induced Hypertension, Vascular Dysfunction, And End-organ Damage
    Authors: Costa, RM;Cerqueira, DM;Bruder-Nascimento, A;Alves, JV;Awata, WAC;Singh, S;Kufner, A;Cifuentes-Pagano, E;Pagano, PJ;Ho, J;Bruder-Nascimento, T;
    bioRxiv : the preprint server for biology  2023-09-23
  29. Critical requirement of SOS1 for tumor development and microenvironment modulation in KRASG12D-driven lung adenocarcinoma
    Authors: Baltanás, FC;García-Navas, R;Rodríguez-Ramos, P;Calzada, N;Cuesta, C;Borrajo, J;Fuentes-Mateos, R;Olarte-San Juan, A;Vidaña, N;Castellano, E;Santos, E;
    Nature communications  2023-09-20
  30. Epicutaneous Sensitization to the Phytocannabinoid ?-Caryophyllene Induces Pruritic Inflammation
    Authors: Inan, S;Ward, SJ;Baltazar, CT;Peruggia, GA;Javed, E;Nayak, AP;
    International journal of molecular sciences  2023-09-20
  31. Contrasting roles of different mismatch repair proteins in basal-like breast cancer
    Authors: Mo, J;Borcherding, N;Jo, S;Tithi, TI;Cho, E;Cash, KE;Honda, M;Wang, L;Ahmed, KK;Weigel, R;Spies, M;Kolb, R;Zhang, W;
    bioRxiv : the preprint server for biology  2023-09-13
  32. The Presence of Pre-Existing Endometriotic Lesions Promotes the Growth of New Lesions in the Peritoneal Cavity
    Authors: Mihai, IT;Rudzitis-Auth, J;Menger, MD;Laschke, MW;
    International journal of molecular sciences  2023-09-08
  33. Fiber deprivation and microbiome-borne curli shift gut bacterial populations and accelerate disease in a mouse model of Parkinson's disease
    Authors: Schmit, KJ;Garcia, P;Sciortino, A;Aho, VTE;Pardo Rodriguez, B;Thomas, MH;Gérardy, JJ;Bastero Acha, I;Halder, R;Cialini, C;Heurtaux, T;Ostahi, I;Busi, SB;Grandmougin, L;Lowndes, T;Singh, Y;Martens, EC;Mittelbronn, M;Buttini, M;Wilmes, P;
    Cell reports  2023-09-06
  34. Heterotypic Cell Culture from Mouse Bone Marrow under Simulated Microgravity: Lessons for Stromal Lineage Functions
    Authors: Markina, E;Tyrina, E;Ratushnyy, A;Andreeva, E;Buravkova, L;
    International journal of molecular sciences  2023-09-06
  35. Naegleria fowleri Extracellular Vesicles Induce Proinflammatory Immune Responses in BV-2 Microglial Cells
    Authors: Lê, HG;Kang, JM;Võ, TC;Yoo, WG;Na, BK;
    International journal of molecular sciences  2023-09-03
  36. CUL4B functions as a tumor suppressor in KRAS-driven lung tumors by inhibiting the recruitment of myeloid-derived suppressor cells
    Authors: Liu, X;Tian, F;Cui, J;Gong, L;Xiang, L;Fan, B;Liu, S;Zhan, J;Zhou, Y;Jiang, B;Wang, M;Sun, G;Gong, Y;Zou, Y;
    Oncogene  2023-08-31
  37. Red cabbage juice-mediated gut microbiota modulation improves intestinal epithelial homeostasis and ameliorates colitis
    Authors: Jean Wilson, E;Sirpu Natesh, N;Ghadermazi, P;Pothuraju, R;Shanmugam, M;Prajapati, DR;Pandey, S;Kaifi, JT;Dodam, JR;Bryan, J;Lorson, CL;Watrelot, AA;Foster, JM;Mansel, TJ;Joshua Chan, SH;Batra, SK;Subbiah, J;Rachagani, S;
    bioRxiv : the preprint server for biology  2023-08-24
  38. Pyruvate dehydrogenase operates as an intramolecular nitroxyl generator during macrophage metabolic reprogramming
    Authors: Palmieri, EM;Holewinski, R;McGinity, CL;Pierri, CL;Maio, N;Weiss, JM;Tragni, V;Miranda, KM;Rouault, TA;Andresson, T;Wink, DA;McVicar, DW;
    Nature communications  2023-08-22
  39. Progranulin Maintains Blood Pressure and Vascular Tone Dependent on EphrinA2 and Sortilin1 Receptors and Endothelial Nitric Oxide Synthase Activation
    Authors: Bruder-Nascimento, A;Awata, WMC;Alves, JV;Singh, S;Costa, RM;Bruder-Nascimento, T;
    Journal of the American Heart Association  2023-08-15
  40. Tumor-derived interleukin-1 receptor antagonist exhibits immunosuppressive functions and promotes pancreatic cancer
    Authors: Fan, YC;Fong, YC;Kuo, CT;Li, CW;Chen, WY;Lin, JD;Bürtin, F;Linnebacher, M;Bui, QT;Lee, KD;Tsai, YC;
    Cell & bioscience  2023-08-10
  41. Adenosine metabolized from extracellular ATP ameliorates organ injury by triggering A2BR signaling
    Authors: Kelestemur, T;Németh, ZH;Pacher, P;Beesley, J;Robson, SC;Eltzschig, HK;Haskó, G;
    Respiratory research  2023-07-13
  42. A novel co-culture model for investigation of the effects of LPS-induced macrophage-derived cytokines on brain endothelial cells
    Authors: Yang, J;Li, Y;Bhalla, A;Maienschein-Cline, M;Fukuchi, KI;
    PloS one  2023-07-13
  43. An LRP1-binding motif in cellular prion protein replicates cell-signaling activities of the full-length protein
    Authors: Mantuano, E;Zampieri, C;Azmoon, P;Gunner, CB;Heye, KR;Gonias, SL;
    JCI insight  2023-06-27
  44. Anti-inflammatory effects of Allium cepa L. peel extracts via inhibition of JAK-STAT pathway in LPS-stimulated RAW264.7 cells
    Authors: Lee, HS;Kwon, YJ;Seo, EB;Kim, SK;Lee, H;Lee, JT;Chang, PS;Choi, YJ;Lee, SH;Ye, SK;
    Journal of ethnopharmacology  2023-06-27
  45. Optineurin deficiency impairs autophagy to cause interferon beta overproduction and increased survival of mice following viral infection
    Authors: Fukushi, M;Ohsawa, R;Okinaka, Y;Oikawa, D;Kiyono, T;Moriwaki, M;Irie, T;Oda, K;Kamei, Y;Tokunaga, F;Sotomaru, Y;Maruyama, H;Kawakami, H;Sakaguchi, T;
    PloS one  2023-06-23
  46. Inflammatory and alternatively activated macrophages independently induce metaplasia but cooperatively drive pancreatic precancerous lesion growth
    Authors: Geou-Yarh Liou, Alicia K. Fleming Martinez, Heike R. Döppler, Ligia I. Bastea, Peter Storz
    iScience  2023-06-16
  47. p53 inhibitor iASPP is an unexpected suppressor of KRAS and inflammation-driven pancreatic cancer
    Authors: Miller, P;Akama-Garren, EH;Owen, RP;Demetriou, C;Carroll, TM;Slee, E;Al Moussawi, K;Ellis, M;Goldin, R;O'Neill, E;Lu, X;
    Cell death and differentiation  2023-06-03
  48. MPC-n (IgG) improves long-term cognitive impairment in the mouse model of repetitive mild traumatic brain injury
    Authors: Zhang, C;Wei, C;Huang, X;Hou, C;Liu, C;Zhang, S;Zhao, Z;Liu, Y;Zhang, R;Zhou, L;Li, Y;Yuan, X;Zhang, J;
    BMC medicine  2023-05-30
  49. Tumour extracellular vesicles and particles induce liver metabolic dysfunction
    Authors: Wang, G;Li, J;Bojmar, L;Chen, H;Li, Z;Tobias, GC;Hu, M;Homan, EA;Lucotti, S;Zhao, F;Posada, V;Oxley, PR;Cioffi, M;Kim, HS;Wang, H;Lauritzen, P;Boudreau, N;Shi, Z;Burd, CE;Zippin, JH;Lo, JC;Pitt, GS;Hernandez, J;Zambirinis, CP;Hollingsworth, MA;Grandgenett, PM;Jain, M;Batra, SK;DiMaio, DJ;Grem, JL;Klute, KA;Trippett, TM;Egeblad, M;Paul, D;Bromberg, J;Kelsen, D;Rajasekhar, VK;Healey, JH;Matei, IR;Jarnagin, WR;Schwartz, RE;Zhang, H;Lyden, D;
    Nature  2023-05-24
  50. Myeloid Cell Derived IL1? Contributes to Pulmonary Vascular Remodeling in Heart Failure with Preserved Ejection Fraction
    Authors: Agrawal, V;Kropski, JA;Gokey, JJ;Kobeck, E;Murphy, M;Murray, KT;Fortune, NL;Moore, CS;Meoli, DF;Monahan, K;Su, YR;Blackwell, T;Gupta, DK;Talati, MH;Gladson, S;Carrier, EJ;West, JD;Hemnes, AR;
    bioRxiv : the preprint server for biology  2023-05-18
  51. Progerinin, an Inhibitor of Progerin, Alleviates Cardiac Abnormalities in a Model Mouse of Hutchinson-Gilford Progeria Syndrome
    Authors: Kang, SM;Seo, S;Song, EJ;Kweon, O;Jo, AH;Park, S;Woo, TG;Kim, BH;Oh, GT;Park, BJ;
    Cells  2023-04-24
  52. Irradiation plus myeloid-derived suppressor cell-targeted therapy for overcoming treatment resistance in immunologically cold urothelial carcinoma
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    Eur. J. Immunol., 2011-05-27;41(7):2000-9.  2011-05-27
  386. Involvement of adenosine A2A receptors in engulfment-dependent apoptotic cell suppression of inflammation.
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    J. Immunol., 2011-05-18;186(12):7144-55.  2011-05-18
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    Am. J. Pathol., 2011-05-07;179(1):180-8.  2011-05-07
  388. TNF-alpha promotes LPA1- and LPA3-mediated recruitment of leukocytes in vivo through CXCR2 ligand chemokines.
    Authors: Zhao C, Sardella A, Chun J, Poubelle PE, Fernandes MJ, Bourgoin SG
    J. Lipid Res., 2011-04-26;52(7):1307-18.  2011-04-26
  389. Tumors Suppress In Situ Proliferation of Cytotoxic T Cells by Promoting Differentiation of Gr-1+ Conventional Dendritic Cells through IL-6.
    Authors: Diao J, Zhao J, Winter E
    J. Immunol., 2011-03-23;186(9):5058-67.  2011-03-23
  390. Reduced VEGF production, angiogenesis, and vascular regrowth contribute to the antitumor properties of dual mTORC1/mTORC2 inhibitors.
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FAQs

  1. Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

    • The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

View all Proteome Profiler Antibody Array FAQs
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Reviews for Proteome Profiler Mouse Cytokine Array Kit, Panel A

Average Rating: 4.8 (Based on 36 Reviews)

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Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Mary-Kate Hayward on 04/12/2024

Performed well for mammary tumor lysates

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Khuynh Nguyen on 08/15/2023

The kit works very well

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/25/2023
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Andres Ocampo on 10/11/2022

Mouse Cytokine Antibody Array was used in serum from Prolactin Receptor wild type baby mice after the mother's high-fat diet feeding for 21 days of lactation, and results were compared with Control Diet (CD). A low-level pro-inflammatory mechanism could be confirmed due to the modulation of inflammation-associated cytokines.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 03/01/2022
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Martin Torres on 10/01/2021

The array was used to test the anti-inflammatory potential from Octopus vulgaris ink extracts on LPS-stimulated RAW 264.7 cells. Results obtained were in agreement with other biochemical and in vitro test. We have used this kit in several LPS-challenged murine macrophages. Fig. A shows the expression level of each cytokine against the control (LPS only-treated macrophages); B shows a bioinformatic arrangement of the modulated cytokines, and C shows a predictive analysis from the results obtained in this kit.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Martin Torres on 09/23/2021

Pro and anti-inflammatory cytokines were evaluated in RAW264.7 macrophages treated with LPS (1 microgram/mL) and a South American berry (ABJ). Results obtained showed the modulation of several cytokines indicated in this kit. We have conducted several evaluations of these cytokines using this kit and results never disappoint. What we like the most is that this assay allows the evaluation of several cytokines that would take a lot of time by conventional techniques such as ELISA or Western Blot.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 09/23/2021

20 ul serum was used to perform the experiment.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 08/31/2021
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anil Singh on 08/25/2021

mouse serum 200ul used for cancer model

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Gaurav Kumar on 08/17/2021

Used serum to see the role of gene in pavreatic cancer

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Allison Scarpitti on 05/10/2021

Phenomenal results, consistent performance and excellent translation to use with Streptavidin-800. On step 11, I swapped the Streptavidin-HRP for a 1:2,000 ratio of Streptavidin-800. Then followed with the wash steps and the result is as posted.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 03/06/2021
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By jinxiu PAN on 02/24/2020

150UL SERUM WAS USED

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/12/2020

mouse tissues

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/12/2020

serum from knock-out mice

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/08/2020
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 12/10/2019

Followed protocol exactly, 10 min exposure on film wasn't great, boosted to 20 min exposure, still not good. 5 min on digital imager and positive control spots were saturated! Much more sensitive!

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 06/06/2019

T cells isolated and stimulated with various agonists.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 04/05/2019

good!

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Ivan Luzardo on 03/13/2019

This array was used to measure the modulation of inflammation-associated cytokines in LPS-stimulated RAW264.7 macrophages treated with several doses of the non-digestible fraction from a corn/bean snack.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Brian Englar on 02/07/2019
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 12/26/2018
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Ivan Luzardo on 12/11/2018
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 11/11/2018

High quality results with simple and straightforward protocol. No optimization needed.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 07/19/2018

instructions easy to follow. I needed a little longer than the 10 minute exposure due to low protein concentrations from my very small tissues.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 04/26/2018
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Adam Guess on 10/20/2017
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 09/27/2017

Very sensitive cytokine assay!

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 07/14/2017

performed on cell conditoned media from mouse fibroblasts

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Michelle Chen on 05/10/2017
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Camila Idelí Morales Fénero on 03/15/2017

Assay tested in 200 ug of protein from pool of intestines of 10 dpf zebrafish larvae.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/27/2017

Mouse BALF samples from pooled control and treatment groups in 2 separate arrays to determine the altered cytokine response.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 01/27/2017

Bronchoalveolar lavage fluid from C57BL/6J mouse

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 10/06/2016
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Kihong Lim on 09/29/2016
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006