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Rat TIMP-1 Antibody

Catalog #: AF580
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF580
AF580-SP
Detection of TIMP‑1 in Rat Brain.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat TIMP-1 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) TIMP‑1 and recombinant mouse TIMP-1 is observed and less than 1% cross-reactivity with rhTIMP-2, rhTIMP-3, and rhTIMP-4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat TIMP-1
Cys24-Ala217
Accession # P30120
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat TIMP‑1 (Catalog # 580-RT)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed paraffin-embedded sections of Rat Brain.
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Rat TIMP‑1 (Catalog # 580-RT), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Detection of TIMP‑1 in Rat Brain. TIMP‑1 was detected in perfusion fixed paraffin-embedded sections of Rat Brain using Goat Anti-Rat TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF580) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIMP-1

Tissue inhibitors of metalloproteinases (TIMPs) are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family: TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 32‑34 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.

Long Name
Tissue Inhibitors of Metalloproteinases 1
Entrez Gene IDs
7076 (Human); 21857 (Mouse); 116510 (Rat)
Alternate Names
CLGI; Collagenase inhibitor; collagenase inhibitor); EPATIMP-1; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; TIMP1; TIMP-1; TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity; Tissue inhibitor of metalloproteinases 1

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Citations for Rat TIMP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Secretome Analysis of Mesenchymal Stem Cell Factors Fostering Oligodendroglial Differentiation of Neural Stem Cells In Vivo
    Authors: Iria Samper Agrelo, Jessica Schira-Heinen, Felix Beyer, Janos Groh, Christine Bütermann, Veronica Estrada et al.
    International Journal of Molecular Sciences
  2. Functional in vivo assessment of stem cell-secreted pro-oligodendroglial factors
    Authors: Patrick Schira-Heinen, Iria Samper Schira-Heinen, IriaSamper Estrada, Veronica Estrada
    Neural Regeneration Research
  3. CCN3/NOV small interfering RNA enhances fibrogenic gene expression in primary hepatic stellate cells and cirrhotic fat storing cell line CFSC
    Authors: Erawan Borkham-Kamphorst, Claudia R. van Roeyen, Eddy Van de Leur, Jürgen Floege, Ralf Weiskirchen
    Journal of Cell Communication and Signaling
  4. Myelin repair is fostered by the corticosteroid medrysone specifically acting on astroglial subpopulations
    Authors: M Silva Oliv, J Schira-Hei, L Reiche, S Han, VCM de Amorim, I Lewen, J Gruchot, P Göttle, R Akkermann, K Azim, P Küry
    EBioMedicine, 2022-08-08;83(0):104204.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: IHC
  5. TIMP-1 inhibits the proteolytic processing of Reelin in experimental epilepsy.
    Authors: Tinnes, Stefanie, Ringwald, Julia, Haas, Carola A
    FASEB J, 2013-03-14;27(7):2542-52.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. Systemic delivery of TNF-related apoptosis-inducing ligand (TRAIL) elevates levels of tissue inhibitor of metalloproteinase-1 (TIMP-1) and prevents type 1 diabetes in nonobese diabetic mice.
    Authors: Kang S, Park EJ, Joe Y, Seo E, Park MK, Seo SY, Chung HY, Yoo YH, Kim DK, Lee HJ
    Endocrinology, 2010-11-03;151(12):5638-46.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Platelet-derived growth factor isoform expression in carbon tetrachloride-induced chronic liver injury.
    Authors: Borkham-Kamphorst E, Kovalenko E, van Roeyen CR, Gassler N, Bomble M, Ostendorf T, Floege J, Gressner AM, Weiskirchen R
    Lab. Invest., 2008-07-28;88(10):1090-100.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Effect of VEGF-A on expression of profibrotic growth factor and extracellular matrix genes in the retina.
    Authors: Kuiper EJ, Hughes JM, Van Geest RJ, Vogels IM, Goldschmeding R, Van Noorden CJ, Schlingemann RO, Klaassen I
    Invest. Ophthalmol. Vis. Sci., 2007-09-01;48(9):4267-76.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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