Human/Cynomolgus Monkey B7-H6 Antibody

Catalog #: MAB10162 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10162-100
MAB10162-SP

Detection of Cynomolgus Monkey B7‑H6 by Western Blot.

3 Images

All B7-H6 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Cynomolgus Monkey B7-H6 Antibody Summary

Species Reactivity

Human, Cynomolgus Monkey

Specificity

Detects human and cynomolgus monkey B7‑H6 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1002910

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived Cynomolgus Monkey B7‑H6
Asp25-Ser262

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Cynomolgus Monkey B7‑H6 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line mock transfected or transfected with cynomologus monkey B7-H6. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Cynomolgus Monkey B7-H6 Monoclonal Antibody (Catalog # MAB10162) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for B7-H6 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

View Larger

Detection of B7-H6 in HEK293 Human Cell Line Transfected with Human B7-H6 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with Human B7-H6 and eGFP was stained with (A) Mouse Anti-Human/Cynomolgus Monkey B7-H6 Monoclonal Antibody (Catalog # MAB10162) or (B) Mouse IgG2B isotype control antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

View Larger

Detection of B7-H6 in HEK293 Human Cell Line Transfected with Cynomologus Monkey B7-H6 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with Cynomolgus Monkey B7-H6 and eGFP was stained with (A) Mouse Anti-Human/Cynomolgus Monkey B7-H6 Monoclonal Antibody (Catalog # MAB10162) or (B) Mouse IgG2B isotype control antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-H6

B7-H6 is a 55 kDa glycosylated member of the B7 family of immune costimulatory proteins (1, 2). Mature B7-H6 consists of an extracellular domain (ECD) that with one Ig-like V domain and one Ig-like C1 domain, a transmembrane segment, and a cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, cynomolgous B7-H6 shares 81%, 81%, 77% and 80% amino acid sequence identity with chimpanzee, orangutan, gibbon, and human B7-H6, respectively. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). B7-H6 is up-regulated on inflammatory monocytes and neutrophils, and a 30 kDa soluble fragment can be released by ADAM-10 or ADAM-17 mediated shedding (8, 9). The expression of B7-H6 on tumor cells correlates with cancer progression and patient's survival in human ovarian cancer (10).

References