Human GLP-1 Antibody

Catalog #: MAB12491 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB12491-100
MAB12491-SP

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Human GLP-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GLP-1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 970307

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human GLP-1 synthetic peptide
Accession # P01275

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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GLP-1/Glucagon in Human Pancreas. GLP-1/Glucagon was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human GLP-1 Monoclonal Antibody (Catalog # MAB12491) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLP-1

Glucagon is a preprotein which is cleaved into four distinct peptides, including the hormone GLP-1 (aa 98-128). GLP-1 is a secreted hormone with multiple effects upon the intestine (gastric motility), pancreas (glucose dependent insulin release) and hypothalamic pituitary axis (modulates LH, THS, CRH, ocytocin and vasopressin secretion). It also affects plasma glucagon levels. GLP-1 is expressed in enteroendocrine L cells and neurons of the caudal brainstem which project to the forbain, the amygdala and the hypothalamus. Recent studies show pancreatic intra-islet GLP-1 expression, which is regulated by cytokines, hyperglycemia and cell injury.

Long Name

Glucagon-like Peptide 1

Entrez Gene IDs

2641 (Human)

Alternate Names

glicentin-related polypeptide; GLP1; GLP-1; glucagon-like peptide 1; GRPP