Human IL-36 alpha /IL-1F6 Antibody

Catalog #: MAB1078
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1078
MAB1078-SP

Detection of Human IL‑36 alpha /IL‑1F6 by Western Blot.

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Human IL-36 alpha /IL-1F6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑36 alpha /IL‑1F6 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-1 receptor antagonist, rhIL-18, rhIL-36Ra, rhIL-36 beta or recombinant mouse IL-36 alpha is observed.

Source

Monoclonal Mouse IgG_2B Clone # 162122

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IL‑36 alpha /IL‑1F6
Met1-Phe158
Accession # Q9UHA7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IL‑36 alpha /IL‑1F6 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-36a/IL-1F6. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-36a/IL-1F6 Monoclonal Antibody (Catalog # MAB1078) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-36a/IL-1F6 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36 alpha/IL-1F6

Human interleukin 1 family member #6 [IL-1F6; also IL-36 alpha and FIL-1 epsilon (epsilon)] is a member of the IL-1 family of proteins (1‑3, 6). IL-1 family members include IL-1 beta, IL-1 alpha, IL‑1ra, IL-18 and IL-1F5 through F10 (4). All family members show a 12 beta -strand, beta -trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-36 alpha is synthesized as a 158 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (1‑3). It appears to be actively secreted (1). When found in cell lysate, it presents as an 18 kDa monomer (2). Human to mouse, full length IL-36 alpha has 54% aa identity. Within the family, IL-36 alpha is 30% aa identical to IL-1ra, and 27%, 31%, 36%, 46%, 57% and 28% aa identical to IL-1 beta, IL-36Ra, IL-1F7, IL-36 beta, IL-36 gamma, and IL-1F10, respectively. Cells reported to express IL-36 alpha include monocytes, B cells and T cells (1, 4). Notably, IL-36 alpha is the only novel IL-1 family member found to be expressed on T-cells. The receptor for IL-36 alpha is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (5). Recombinant IL-36 alpha, along with IL-36 beta and IL-36 gamma, has been shown to act as an agonist by activating the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner. This suggests that IL-36 alpha may signal in a similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.

References

Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
Dunn, E. et al. (2001) Trends Immunol. 22:533.
Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Dinarello, C. et al. (2010) Nat. Immunol. 11:973.

Long Name

Interleukin 36 alpha/Interleukin 1 Family 6

Entrez Gene IDs

27179 (Human); 54448 (Mouse)

Alternate Names

FIL1 epsilon; FIL1; FIL1(EPSILON); FIL1E; IL-1 epsilon; IL1(EPSILON); IL1E; IL-1E; IL-1F6 (FIL-1-epsilon); IL1F6; IL-1F6; IL36 alpha; IL-36 alpha; IL36A; interleukin 1 family, member 6 (epsilon); interleukin 1, epsilon; Interleukin 36, Alpha; Interleukin-1 epsilon; interleukin-1 family member 6; Interleukin-36 Alpha

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Citation for Human IL-36 alpha /IL-1F6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Interleukin-1 family cytokines and their regulatory proteins in normal pregnancy and pre-eclampsia.
Authors: Southcombe J, Redman C, Sargent I, Granne I
Clin Exp Immunol, 2015-06-29;181(3):480-90.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot