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Human MMP-9 Antibody

Catalog #: AF911
24 Citations 5 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF911
AF911-SP
MMP‑9 in NS0 Mouse Cell Line.
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Product Details
Citations (24)
FAQs
Supplemental Products
Reviews (5)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MMP-9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human and mouse MMP-9 in Western blots, dot blots, and human MMP-9 by immunocytochemistry. In Western blots, less than 1% cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -10, -12, and -13 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human MMP‑9 Western Blot Standard (Catalog # WBC018)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑9 (Catalog # 911-MP), see our available Western blot detection antibodies
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry MMP‑9 antibody in NS0 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

MMP‑9 in NS0 Mouse Cell Line. MMP‑9 was detected in immersion fixed NS0 mouse myeloma cell line transfected with MMP-9 using 5 µg/mL Human MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry MMP-9 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

MMP‑9 in Human Ovarian Cancer Tissue. MMP-9 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry MMP-9 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

MMP‑9 in MCF‑7 Human Cell Line. MMP-9 was detected in immersion fixed MCF-7 human breast cancer cell line using Human MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry MMP-9 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

MMP‑9 in Human Ovarian Cancer Tissue. MMP-9 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Proximity Ligation Assay Detection of Human MMP-9 by Proximity Ligation Assay View Larger

Detection of Human MMP-9 by Proximity Ligation Assay Univariate survival analysis of disease free survival (DFS) in plasma for all 465 patients. A) MMP-9:TIMP-1 measured by ELISA. B) MMP-9:TIMP-1 measured by PLA. Patients are divided into four groups of equal size (Q1-Q4) according to increasing plasma MMP-9:TIMP-1 levels; Q1 being the group with the lowest level. Image collected and cropped by CiteAb from the following open publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-598), licensed under a CC-BY license. Not internally tested by R&D Systems.

Proximity Ligation Assay Detection of Human MMP-9 by Proximity Ligation Assay View Larger

Detection of Human MMP-9 by Proximity Ligation Assay Univariate survival analysis of disease free survival (DFS) in plasma for all 465 patients. A) MMP-9:TIMP-1 measured by ELISA. B) MMP-9:TIMP-1 measured by PLA. Patients are divided into four groups of equal size (Q1-Q4) according to increasing plasma MMP-9:TIMP-1 levels; Q1 being the group with the lowest level. Image collected and cropped by CiteAb from the following open publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-598), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-9

Pro-MMP-9 is an inactive proenzyme secreted by multiple cell types. The N-terminal pro region is proteolytically removed, resulting in active MMP-9 which can degrade collagens and elastin as well as several non-ECM molecules.

Long Name
Matrix Metalloproteinase 9
Entrez Gene IDs
4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)
Alternate Names
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase

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Citations for Human MMP-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Ubiquitin-specific protease TRE17/USP6 promotes tumor cell invasion through the regulation of glycoprotein CD147 intracellular trafficking
    Authors: Y Ogura, N Ohbayashi, Y Kanaho, A Kawaguchi, Y Funakoshi
    The Journal of Biological Chemistry, 2022-08-02;0(0):102335.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Zymography
  2. ADAM8-Dependent Extracellular Signaling in the Tumor Microenvironment Involves Regulated Release of Lipocalin 2 and MMP-9
    Authors: L Cook, M Sengelmann, B Winkler, C Nagl, S Koch, U Schlomann, EP Slater, MA Miller, EP von Strand, B Dörsam, C Preu beta er, JW Bartsch
    International Journal of Molecular Sciences, 2022-02-10;23(4):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Splice-switching of the insulin receptor pre-mRNA alleviates tumorigenic hallmarks in rhabdomyosarcoma.
    Authors: Safiya K, Matias M, Daniel J et al.
    NPJ Precis Oncol.
  4. Acute elevation of interleukin 6 and matrix metalloproteinase 9 during the onset of pituitary apoplexy in Cushing’s disease
    Authors: Takako Araki, Jutarat Sangtian, Darin Ruanpeng, Ramachandra Tummala, Brent Clark, Lynn Burmeister et al.
    Pituitary
  5. CD44 modulates metabolic pathways and altered ROS-mediated Akt signal promoting cholangiocarcinoma progression
    Authors: M Thanee, H Dokduang, Y Kittirat, J Phetcharab, P Klanrit, A Titapun, N Namwat, N Khuntikeo, A Wangwiwats, H Saya, W Loilome
    PLoS ONE, 2021-03-29;16(3):e0245871.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. miR‑205 suppresses cell migration, invasion and EMT of colon cancer by targeting mouse double minute 4
    Authors: Yujing Fan, Kuanyu Wang
    Molecular Medicine Reports
  7. EDTA/gelatin zymography method to identify C1s versus activated MMP-9 in plasma and immune complexes of patients with systemic lupus erythematosus
    Authors: E Ugarte-Ber, E Martens, L Boon, J Vandooren, D Blockmans, P Proost, G Opdenakker
    J. Cell. Mol. Med., 2018-10-24;23(1):576-585.
  8. Progranulin modulates cholangiocarcinoma cell proliferation, apoptosis, and motility via the PI3K/pAkt pathway
    Authors: Minerva Daya, Watcharin Loilome, Anchalee Techasen, Malinee Thanee, Prakasit Sa-Ngiamwibool, Attapol Titapun et al.
    OncoTargets and Therapy
  9. Interleukin-6 as a potential molecular target in esophageal squamous cell carcinoma
    Authors: ZHI-FEI ZHAO, JIAN-XIONG LI, RUI YE, XUAN WU, LING-LING GAO, BAO-LONG NIU
    Oncology Letters
  10. TREM-1 Mediates Inflammatory Injury and Cardiac Remodeling Following Myocardial Infarction
    Authors: Amir Boufenzer, Jérémie Lemarié, Tabassome Simon, Marc Derive, Youcef Bouazza, Nguyen Tran et al.
    Circulation Research
  11. RAGE mediates S100A7-induced breast cancer growth and metastasis by modulating the tumor microenvironment.
    Authors: Nasser M, Wani N, Ahirwar D, Powell C, Ravi J, Elbaz M, Zhao H, Padilla L, Zhang X, Shilo K, Ostrowski M, Shapiro C, Carson W, Ganju R
    Cancer Res, 2015-01-08;75(6):974-85.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  12. Anti-vascular endothelial growth factor therapy-induced glioma invasion is associated with accumulation of Tie2-expressing monocytes
    Authors: Konrad Gabrusiewicz, Dan Liu, Nahir Cortes-Santiago, Mohammad B. Hossain, Charles A. Conrad, Kenneth D. Aldape et al.
    Oncotarget
  13. Plasma levels of the MMP-9:TIMP-1 complex as prognostic biomarker in breast cancer: a retrospective study.
    Authors: Thorsen S, Christensen S, Wurtz S, Lundberg M, Nielsen B, Vinther L, Knowles M, Gee N, Fredriksson S, Moller S, Brunner N, Schrohl A, Stenvang J
    BMC Cancer, 2013-12-13;13(0):598.
    Species: Human
    Sample Types: Plasma
    Applications: Proximity Ligation Assay (PLA)
  14. Pivotal Role of Matrix Metalloproteinase 13 in Extracellular Matrix Turnover in Idiopathic Pulmonary Fibrosis
    Authors: Takwi Nkyimbeng, Clemens Ruppert, Takayuki Shiomi, Bhola Dahal, György Lang, Werner Seeger et al.
    PLoS ONE
  15. Up-Regulation of Cyclooxygenase 2 and Matrix Metalloproteinases-2 and -9 in Cutaneous Squamous Cell Carcinoma: Active Role of Inflammation and Tissue Remodeling in Carcinogenesis
    Authors: Jeong-Hoon Lee, Mei Shan Piao, Jee-Young Choi, Sook Jung Yun, Jee-Bum Lee, Seung-Chul Lee
    Annals of Dermatology
  16. Humanin, a cytoprotective peptide, is expressed in carotid atherosclerotic [corrected] plaques in humans.
    Authors: Zacharias DG, Kim SG, Massat AE, Bachar AR, Oh YK, Herrmann J, Rodriguez-Porcel M, Cohen P, Lerman LO, Lerman A
    PLoS ONE, 2012-02-06;7(2):e31065.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Proteolytic action of kallikrein-related peptidase 7 produces unique active matrix metalloproteinase-9 lacking the C-terminal hemopexin domains
    Authors: Vishnu C. Ramani, Gur P. Kaushal, Randy S. Haun
    Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
  18. GDF-15 is an inhibitor of leukocyte integrin activation required for survival after myocardial infarction in mice.
    Authors: Kempf T, Zarbock A, Widera C, Butz S, Stadtmann A, Rossaint J, Bolomini-Vittori M, Korf-Klingebiel M, Napp LC, Hansen B, Kanwischer A, Bavendiek U, Beutel G, Hapke M, Sauer MG, Laudanna C, Hogg N, Vestweber D, Wollert KC
    Nat. Med., 2011-04-24;17(5):581-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  19. TRE17/USP6 oncogene translocated in aneurysmal bone cyst induces matrix metalloproteinase production via activation of NF-kappaB.
    Authors: Ye Y, Pringle LM, Lau AW
    Oncogene, 2010-04-26;29(25):3619-29.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Fibroblast-conditioned media promote human sarcoma cell invasion.
    Authors: Bittner JG, Wilson M, Shah MB, Albo D, Feig BW, Wang TN
    Surgery, 2008-09-14;145(1):42-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  22. Molecular profiling of cervical cancer progression.
    Authors: Hagemann T, Bozanovic T, Hooper S, Ljubic A, Slettenaar VI, Wilson JL, Singh N, Gayther SA, Shepherd JH, Van Trappen PO
    Br. J. Cancer, 2007-01-29;96(2):321-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  23. Differential proteolytic enzyme activity in eosinophilic and neutrophilic asthma.
    Authors: Simpson JL, Scott RJ, Boyle MJ, Gibson PG
    Am. J. Respir. Crit. Care Med., 2005-05-18;172(5):559-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  24. Differential expression and activity of matrix metalloproteinases during flow-modulated vein graft remodeling.
    Authors: Berceli SA, Jiang Z, Klingman NV, Pfahnl CL, Abouhamze ZS, Frase CD, Schultz GS, Ozaki CK
    J. Vasc. Surg., 2004-05-01;39(5):1084-90.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC-P

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Reviews for Human MMP-9 Antibody

Average Rating: 4.2 (Based on 5 Reviews)

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Human MMP-9 Antibody
By Anonymous on 07/13/2018
Application: WB Sample Tested: HeLa cells Species: Human
Human MMP-9 Antibody
By Anonymous on 07/02/2018
Application: WB Sample Tested: Purified Human MMP9,Purified MMP9 Species: Human
Western Blot Human MMP-9 Antibody AF911
Human MMP-9 Antibody
By Balaji Mahender on 12/06/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human
Human MMP-9 Antibody
By Anonymous on 11/10/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used as the detection in an ELISA built to measure MMP-9 in human serum samples. MAB936 was used as the capture antibody in the immunoassay.

ELISA Human MMP-9 Antibody AF911
Human MMP‑9 Antibody
By Anonymous on 02/27/2016
Application: WB Sample Tested: Human cell conditioned medium Species: Human
Western Blot Human MMP‑9 Antibody AF911