Human/Mouse EphB6 Antibody

Name: Human/Mouse EphB6 Antibody
Brand: R&D Systems
SKU: MAB3384
Rating: 5 (1 reviews)

Catalog #: MAB3384 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3384
MAB3384-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse EphB6 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse EphB6 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) EphB6 is observed, 25% cross-reactivity with recombinant human (rh) EphA10 is observed, and no cross-reactivity with rhEphA1, 2, 5, rhEphB3, 4, rmEphA1, 3, 4, 6, 7, 8, rmEphB2, 3, recombinant rat (rr) EphA5, or rrEphB1 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 465323

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human EphB6
Val17-Thr579
Accession # O15197

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human EphB6 Fc Chimera (Catalog # 3384-B6)
Recombinant Mouse EphB6 Fc Chimera (Catalog # 611-B6)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphB6

EphB6, also known as Hep and Mep, is a 110 kDa type I transmembrane glycoprotein in the Eph receptor tyrosine kinase family. EphB6 is expressed in brain, pancreas, thymus, kidney, and on peripheral T cells. Depending on concentration, Ephrin-B2 can either inhibit or promote EphB6 mediated cell adhesion and migration. EphB6 participates in T cell activation, and its level of expression is inversely correlated with tumor aggressiveness in a variety of malignancies. Within the ECD, human EphB6 shares 91% aa sequence identity with mouse and rat EphB6.

Long Name

Eph Receptor B6

Entrez Gene IDs

2051 (Human); 13848 (Mouse)

Alternate Names

EC 2.7.10.1; EPH receptor B6; EphB6; ephrin type-B receptor 6; Hep; HEPTyrosine-protein kinase-defective receptor EPH-6; Mep; MGC129910; MGC129911