Human/Mouse/Rat MP1 Antibody

Catalog #: MAB4367 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4367-SP	Ships in 5-7 Days	25 ug / $149
MAB4367	In Stock	100 ug / $419

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Detection of Human/Mouse/Rat MP1 by Western Blot.

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Human/Mouse/Rat MP1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat MP1 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 448406

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human MP1
Ala2-Ser124
Accession # Q9UHA4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Citations (9)

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat MP1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, Balb/3T3 mouse embryonic fibroblast cell line, NRK rat normal kidney cells, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat MP1 Monoclonal Antibody (Catalog # MAB4367) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MP1 (2 ng) was included. A specific band for MP1 was detected at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MP1

MP1 (MEK Partner 1), gene name LAMTOR3 (late endosomal/lysosomal adaptor and MAPK and MTOR activator 3), was initially identified as a scaffold protein that tethers MEK1 and ERK1 to facilitate their activation. MP1 and other MAPK scaffolds promote phosphorylation efficiency and specificity, localize their bound pathway components to particular subcellular sites, and serve as nodes of signal integration for regulation of MAPK pathways by other signaling events. With the assistance of adaptor protein p14, the bound MP1 complex localizes to late endosomes and is thought to regulate endosomal traffic and cellular proliferation during tissue homeostasis.

Long Name

MEK-binding Protein 1

Entrez Gene IDs

8649 (Human); 56692 (Mouse); 362045 (Rat)

Alternate Names

LAMTOR3; Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3; late endosomal/lysosomal adaptor, MAPK and MTOR activator 3; MAP2K1IP1; Mapbp; MAPK scaffold protein 1; MAPKSP1; MEK binding partner 1; MEK partner 1; MEK-binding partner 1; mitogen-activated protein kinase kinase 1 interacting protein 1; Mitogen-activated protein kinase kinase 1-interacting protein 1; Mitogen-activated protein kinase scaffold protein 1; MP1; PRO0633; Ragulator3