Human/Mouse/Rat PRL-3 Antibody Summary
Ala2-Met173
Accession # O75365
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat PRL‑3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.3 µg/mL of Human/Mouse/Rat PRL-3 Monoclonal Antibody (Catalog # MAB3219) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PRL-3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PRL-3/PTP4A3
Phosphatase of Regenerating Liver-3 (PRL-3), also known as PTP4A3, is an enzyme that removes phosphate modifications from tyrosine residues on proteins. Prenylation at the C-terminal end targets PRL-3 to membrane compartments in the cell, especially to early endosomes and the plasma membrane. Elevated levels of PRL-3 are observed in metastatic colorectal, ovarian, liver and skin cancers. PRL-3 levels may correlate with cell migration rates and tumor invasiveness.
Product Datasheets
Citations for Human/Mouse/Rat PRL-3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
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Phosphatase and Pseudo-Phosphatase Functions of Phosphatase of Regenerating Liver 3 (PRL-3) Are Insensitive to Divalent Metals In Vitro
Authors: Jeffery T. Jolly, Ty C. Cheatham, Jessica S. Blackburn
ACS Omega
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Development and characterization of nanobodies that specifically target the oncogenic Phosphatase of Regenerating Liver-3 (PRL-3) and impact its interaction with a known binding partner, CNNM3
Authors: Smith, CN;Kihn, K;Williamson, ZA;Chow, KM;Hersh, LB;Korotkov, KV;Deredge, D;Blackburn, JS;
PloS one
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
Credentialing and Pharmacologically Targeting PTP4A3 Phosphatase as a Molecular Target for Ovarian Cancer
Authors: John S. Lazo, Elizabeth R. Sharlow, Robert Cornelison, Duncan J. Hart, Danielle C. Llaneza, Anna J. Mendelson et al.
Biomolecules
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Tumor necrosis factor alpha-induced microRNA-18a activates rheumatoid arthritis synovial fibroblasts through a feedback loop in NF-kappaB signaling.
Authors: Trenkmann M, Brock M, Gay R, Michel B, Gay S, Huber L
Arthritis Rheum, 2013-04-01;65(4):916-27.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Phosphatase of regenerating liver-3 as a convergent therapeutic target for lymph node metastasis in esophageal squamous cell carcinoma.
Authors: Ooki A, Yamashita K, Kikuchi S, Sakuramoto S, Katada N, Watanabe M
Int. J. Cancer, 2010-08-01;127(3):543-54.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Expression and prognostic impact of PRL-3 in lymph node metastasis of gastric cancer: its molecular mechanism was investigated using artificial microRNA interference.
Authors: Wang Z, He YL, Cai SR, Zhan WH, Li ZR, Zhu BH, Chen CQ, Ma JP, Chen ZX, Li W, Zhang LJ
Int. J. Cancer, 2008-09-15;123(6):1439-47.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
A selective phosphatase of regenerating liver phosphatase inhibitor suppresses tumor cell anchorage-independent growth by a novel mechanism involving p130Cas cleavage.
Authors: Daouti S, Li WH, Qian H, Huang KS, Holmgren J, Levin W, Reik L, McGady DL, Gillespie P, Perrotta A, Bian H, Reidhaar-Olson JF, Bliss SA, Olivier AR, Sergi JA, Fry D, Danho W, Ritland S, Fotouhi N, Heimbrook D, Niu H
Cancer Res., 2008-02-15;68(4):1162-9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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