Home / Osterix/Sp7 / Human Osterix/Sp7 Antibody

Human Osterix/Sp7 Antibody

Catalog #: MAB7547
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7547
MAB7547-SP
Detection of Human Osterix/Sp7 by Western Blot.
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Product Details
Citations (5)
FAQs
Supplemental Products
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Osterix/Sp7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Osterix/Sp7 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 764704
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Osterix/Sp7
Met19-Leu288
Accession # Q8TDD2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Osterix/Sp7 antibody by Western Blot. View Larger

Detection of Human Osterix/Sp7 by Western Blot. Western blot shows lysates of Saos-2 human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB7547) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Osterix/Sp7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Osterix/Sp7 antibody in Saos-2 Human Cell Line by Immunocytochemistry (ICC). View Larger

Osterix/Sp7 in Saos‑2 Human Cell Line. Osterix/Sp7 was detected in immersion fixed Saos-2 human osteosarcoma cell line using Mouse Anti-Human Osterix/Sp7 Monoclonal Antibody (Catalog # MAB7547) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osterix/Sp7

Osterix, also known as Sp7, is an approximately 45 kDa transcription factor that is required for osteogenesis and bone homeostasis. Osterix cooperates with BMP-6 in the differentiation of osteoblasts from mesenchymal stem cells by regulating the transcription of several genes involved in osteoblast differentiation and function (i.e. SATB2, Collagen V, and SOST). The transcription of Osterix is induced by BMP-2, IGF-I, and parathyroid hormone, while its activity is regulated by Akt and p38 MAPK-mediated phoshorylation. Osterix contains a transactivation domain (aa 141-210) and three zinc finger domains (aa 294-318, aa 324-348, and aa 354-376). Alternative splicing generates a short isoform that lacks the N-terminal 18 amino acids. Within aa 19-288, human Osterix shares 94% aa sequence identity with mouse and rat Osterix.

Long Name
Sp7 Transcription Factor
Entrez Gene IDs
121340 (Human); 170574 (Mouse); 300260 (Rat)
Alternate Names
MGC126598; Osterix; OSX; OSXosterix; Sp7 transcription factor; Sp7; transcription factor Sp7; Zinc finger protein osterix

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Citations for Human Osterix/Sp7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. L-PRF Secretome from Both Smokers/Nonsmokers Stimulates Angiogenesis and Osteoblast Differentiation In Vitro
    Authors: Ríos, S;González, LG;Saez, CG;Smith, PC;Escobar, LM;Martínez, CE;
    Biomedicines
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Western Blot, Immunocytochemistry
  2. Developmentally Engineered Callus Organoid Bioassemblies Exhibit Predictive In Vivo Long Bone Healing
    Authors: Gabriella Nilsson Hall, Luís Freitas Mendes, Charikleia Gklava, Liesbet Geris, Frank P. Luyten, Ioannis Papantoniou
    Advanced Science
  3. Impact of Four Protein Additives in Cryogels on Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells
    Authors: Victor Häussling, Sebastian Deninger, Laura Vidoni, Helen Rinderknecht, Marc Ruo beta, Christian Arnscheidt et al.
    Bioengineering (Basel)
  4. Donor Site Location Is Critical for Proliferation, Stem Cell Capacity, and Osteogenic Differentiation of Adipose Mesenchymal Stem/Stromal Cells: Implications for Bone Tissue Engineering
    Authors: Marie K. Reumann, Caren Linnemann, Romina H. Aspera-Werz, Sigrid Arnold, Manuel Held, Claudine Seeliger et al.
    International Journal of Molecular Sciences
  5. Gold nanoparticles promote osteogenic differentiation of human periodontal ligament stem cells via the p38 MAPK signaling pathway
    Authors: Chenguang Niu, Keyong Yuan, Rui Ma, Li Gao, Wenxin Jiang, Xuchen Hu et al.
    Molecular Medicine Reports

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