Human PTHLH/PTHrP Antibody Summary
Ala37-Arg175
Accession # P12272
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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PTHLH/PTHrP in Human Colon. PTHLH/PTHrP was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human PTHLH/PTHrP Monoclonal Antibody (Catalog # MAB6734) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTHLH/PTHrP
The parathyroid hormone-like hormone (PTHLH), also known as PTHrP, has been recognized in patients suffering from tumors associated with the common neoplastic syndrome humoral hypercalcemia of malignancy (HHM). The 18 kDa protein is a 177 amino acid (aa) precursor that belongs to the parathyroid hormone family. It contains a 24 aa signal sequence followed by a 10 aa propeptide (aa 25-34). The mature chain runs from aa 37-177. Of note, residues 108-129 constitute a nuclear localization signal (NLS) providing evidence that the protein not only acts as a secreted factor, but in an intracrine manner as well. A second isoform is missing aa 176-177, and a 34 aa substitution for aa 176-177 produces a third isoform. There is a strong homology displayed between PTHLH and human parathyroid hormone (PTH) among the first 13 aa. Studies show that the similarity in this area is 67% at the aa and nucleotide levels. Indeed, eight of the first 13 aa of PTHLH and PTH are identical. Human PTHLH and mouse PTHLH share 86% aa sequence identity.
Product Datasheets
Citations for Human PTHLH/PTHrP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures.
Authors: Juhasz T, Szentleleky E, Somogyi CS et al.
Int J Mol Sci.
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EZH2 engages TGFbeta signaling to promote breast cancer bone metastasis via integrin beta1-FAK activation
Authors: L Zhang, J Qu, Y Qi, Y Duan, YW Huang, Z Zhou, P Li, J Yao, B Huang, S Zhang, D Yu
Nature Communications, 2022-05-10;13(1):2543.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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We used this mouse-derived antibody to try western detection. 25ug was dissolved in 10ml TBST. In a variety of lung cancer cell lines, we detected some bands, but the signal was relatively weak. There were some differences between groups after long-term exposure. If you use this For WB experiments with antibodies, it is recommended to increase the detection concentration to about 1:500.
