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Human S1P1/EDG-1 Antibody

Catalog #: MAB2016
11 Citations Datasheet / COA / SDS
Clone 218713 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB2016-SP
MAB2016-500
MAB2016-100
Detection of S1P1/EDG‑1 in CHO Chinese Hamster Cell Line Transfected with Human S1P1/EDG-1 and eGFP by Flow Cytometry.
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Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human S1P1/EDG-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human S1P1/EDG‑1 by flow cytometry.
Source
Monoclonal Mouse IgG2B Clone # 218713
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human S1P1/EDG‑1
Met1-Ser382
Accession # P21453
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of S1P<sub>1</sub>/EDG-1 antibody in CHO Chinese Hamster Cell Line Transfected with Human S1P<sub>1</sub>/EDG-1 and eGFP antibody by Flow Cytometry. View Larger

Detection of S1P1/EDG‑1 in CHO Chinese Hamster Cell Line Transfected with Human S1P1/EDG-1 and eGFP by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with either (A) human S1P1/EDG-1 or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB2016) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Human S1P1/EDG-1 by Flow Cytometry View Larger

Detection of Human S1P1/EDG-1 by Flow Cytometry S1PR1 expression by OECs is dynamically altered by microenvironment.OEC expression of S1PR1 under normoxia and hypoxia was qualitatively confirmed by immunocytochemistry (A). The percentage of cells expressing S1PR1 on the cell surface, as confirmed with flow cytometry, was enhanced under hypoxia as compared to the normoxic control (B). Combined stimuli of S1P and VEGF resulted in greater S1PR1 mRNA production after 24h in normoxia (C). Combined stimuli also resulted in a greater proportion of S1PR1+ OECs under both normoxia and hypoxia (D and E). Scale bars represent 40 μm. Data are mean ± SD (n = 4) and normalized to the average untreated control values for either normoxia or hypoxia (C, E; indicated by dashed line) accordingly. An asterisk indicates statistically significant differences (P<0.05) between conditions in normoxia or hypoxia respectively and N.S. displays no statistically significant difference between conditions. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0123437), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S1P1/EDG-1

S1P1, also known as EDG-1, is a member of the endothelial differentiation gene family. It is a high affinity G protein-coupled receptor for the bioactive lipid, Sphingosine-1-phosphate. S1P1 signaling regulates endothelial cell survival, cytoskeletal remodeling, chemotaxis, and angiogenesis.

Long Name
Endothelial Differentiation Gene 1
Entrez Gene IDs
1901 (Human); 13609 (Mouse)
Alternate Names
CD363 antigen; CD363; CHEDG1; D1S3362; EDG1; EDG-1; EDG1edg-1; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; FLJ58121; S1P receptor 1; S1P receptor Edg-1; S1P1; S1P1ECGF1; S1PR1; sphingosine 1-phosphate receptor 1; sphingosine 1-phosphate receptor EDG1; Sphingosine 1-phosphate receptor Edg-1; sphingosine-1-phosphate receptor 1

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Citations for Human S1P1/EDG-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Development and application of a high-content virion display human GPCR array
    Authors: Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al.
    Nature Communications
  2. Arsenic Trioxide and (-)-Gossypol Synergistically Target Glioma Stem-Like Cells via Inhibition of Hedgehog and Notch Signaling
    Authors: Linder B, Wehle A, Hehlgans S et al.
    Cancers (Basel)
  3. Sphingosine-1-phosphate receptor 1/5 selective agonist alleviates ocular vascular pathologies
    Authors: Nakamura, S;Yamamoto, R;Matsuda, T;Yasuda, H;Nishinaka, A;Takahashi, K;Inoue, Y;Kuromitsu, S;Shimazawa, M;Goto, M;Narumiya, S;Hara, H;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. The GPCR adaptor protein Norbin regulates S1PR1 trafficking and the morphology, cell cycle and survival of PC12 cells
    Authors: Johansen, VBI;Hampson, E;Tsonou, E;Pantarelli, C;Chu, JY;Crossland, L;Okkenhaug, H;Massey, AJ;Hornigold, DC;Welch, HCE;Chetwynd, SA;
    Scientific reports
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. An S1P1 agonist, ASP4058, suppresses intracranial aneurysm through promoting endothelial integrity and blocking macrophage transmigration
    Authors: R Yamamoto, T Aoki, H Koseki, M Fukuda, J Hirose, K Tsuji, K Takizawa, S Nakamura, H Miyata, N Hamakawa, H Kasuya, K Nozaki, Y Hirayama, I Aramori, S Narumiya
    Br. J. Pharmacol., 2017-05-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Hypoxia augments outgrowth endothelial cell (OEC) sprouting and directed migration in response to sphingosine-1-phosphate (S1P).
    Authors: Williams P, Stilhano R, To V, Tran L, Wong K, Silva E
    PLoS ONE, 2015-04-15;10(4):e0123437.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Expression of functional sphingosine-1 phosphate receptor-1 is reduced by B cell receptor signaling and increased by inhibition of PI3 kinase delta but not SYK or BTK in chronic lymphocytic leukemia cells.
    Authors: Till K, Pettitt A, Slupsky J
    J Immunol, 2015-01-28;194(5):2439-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Sphingosine-1-phosphate (S1P) displays sustained S1P1 receptor agonism and signaling through S1P lyase-dependent receptor recycling.
    Authors: Gatfield J, Monnier L, Studer R, Bolli M, Steiner B, Nayler O
    Cell Signal, 2014-04-02;26(7):1576-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. New B-cell CD molecules.
    Authors: Matesanz-Isabel J, Sintes J, Llinas L, de Salort J, Lazaro A, Engel P
    Immunol. Lett., 2010-10-07;134(2):104-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
    Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
    Immunol. Lett., 2010-10-07;134(2):167-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
    Authors: Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C
    FASEB J., 2010-05-19;24(10):3782-95.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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