Human TCF-12/HTF4 Antibody

Catalog #: MAB6928 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6928
MAB6928-SP

Detection of Human TCF-12/HTF4 by Western Blot.

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All TCF-12/HTF4 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TCF-12/HTF4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TCF-12/HTF4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human TCF1, -2, -3, or -5 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 690124

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TCF-12/HTF4
Met108-Glu521
Accession # Q99081

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TCF-12/HTF4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TCF-12/HTF4 Monoclonal Antibody (Catalog # MAB6928) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TCF-12/HTF4 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCF-12/HTF4

TCF-12 (transcription factor 12), also called HTF4, bHLHb20 and HEB, is a 682 amino acid (aa) DNA binding protein of the E protein family of helix-loop-helix (HLH) transcription factors. E proteins, including TCF-12 and E2A, mediate lymphocyte differentiation and also function in neurogenesis. Homo-oligomerization is required for activity; hetero-oligomers are also formed. The region of human TCF-12 used as an immunogen excludes the HLH domain and shares 94% aa sequence identity with mouse and rat TCF-12. Potential isoforms of 706 and 648 aa contain an insertion of 24 aa after aa 396, while isoforms of 648, 624 and 512 aa show alternate N‑terminal sequences that merge with the 682 aa form at aa 75, 75 and 194, respectively.

Long Name

Transcription Factor 12

Entrez Gene IDs

6938 (Human); 21406 (Mouse); 25720 (Rat)

Alternate Names

BHLHB20; bHLHb20E-box-binding protein; Class B basic helix-loop-helix protein 20; HEB; HEBDNA-binding protein HTF4; helix-loop-helix transcription factor 4; HsT17266; HTF4; HTF4TCF-12; TCF12; TCF-12; transcription factor 12; Transcription factor HTF-4