Human Tryptase alpha /TPS1 Antibody

Catalog #: AF2370 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2370
AF2370-SP

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Tryptase alpha /TPS1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Tryptase alpha /TPS1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 60% cross‑reactivity with recombinant human (rh) TPSB2 is observed and 10% cross-reactivity with rhTPSG1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Tryptase alpha /TPS1
Ile31-Pro275
Accession # AAA86934

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Tryptase alpha /TPS1

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Tryptase alpha /TPS1, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptase alpha/TPS1

Tryptases are serine proteases with trypsin-like specificity. Together with chymases and Cathepsin G, tryptases are important players in mast cell mediation of inflammatory and allergic responses. Tryptase beta-1, also known as mast cell protease 7 (MCPT7), exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of a diverse array of protease inhibitors in plasma. Tryptase epsilon, also known as brain-specific serine protease 4 (BSSP-4) and brain serine protease 2 (BSP-2), is encoded by the PRSS22 gene and preferentially expressed in epithelium-rich tissues such as lung and eye. Tryptase gamma-1, also called transmembrane tryptase, is encoded by TPSG1, one of many serine protease genes clustered on human chromosome 16p13.3.

Entrez Gene IDs

7177 (Human)

Alternate Names

alpha-Tryptase; EC:3.4.21.59; epididymis secretory sperm binding protein; mast cell alpha II tryptase; mast cell beta I tryptase; mast cell tryptase; TPS1; TPS2; TPSAB1; TPSB1; TPSB2; Tryptase 1; tryptase alpha II; Tryptase alpha; Tryptase alpha-1; tryptase beta I; tryptase beta-1; Tryptase beta-2; Tryptase I; tryptase II; tryptase-1; tryptase-2; tryptase-I; tryptase-III