NucA nuclease Antibody Summary
Met1-Asn266
Accession # P13717
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of NucA nuclease by Western Blot. Western blot shows recombinant NucA nuclease protein. PVDF membrane was probed with 2 µg/mL of Mouse Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100633) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for NucA nuclease at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of NucA nuclease by Simple WesternTM. Simple Western lane view shows recombinant NucA nuclease protein, loaded at 0.2 mg/mL. A specific band was detected for NucA nuclease at approximately 35 kDa (as indicated) using 10 µg/mL of Mouse Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100633). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NucA nuclease
Product Datasheets
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