Home / IL-6 / Porcine IL-6 Antibody

Porcine IL-6 Antibody

Catalog #: AF686
9 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF686
AF686-SP
Cell Proliferation Induced by IL‑6 and Neutralization by Porcine IL‑6 Antibody.
1 Image
Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Porcine IL-6 Antibody Summary

Species Reactivity
Porcine
Specificity
Detects porcine IL-6 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human IL‑6, recombinant mouse IL‑6, and recombinant rat IL‑6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant porcine IL-6
Pro29-Met212
Accession # P26893
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Porcine IL‑6 (Catalog # 686-PI)

Porcine IL-6 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Porcine IL‑6 Biotinylated Antibody (Catalog # BAF686)

Standard: Recombinant Porcine IL-6 Protein (Catalog # 686-PI)

Neutralization
Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line [Nordan, R.P. and M. Potter (1986) Science 233:566]. The Neutralization Dose (ND50) is typically 0.15-0.6 µg/mL in the presence of 12.5 ng/mL Recombinant Porcine IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑6 and Neutralization by Porcine IL‑6 Antibody. View Larger

Cell Proliferation Induced by IL‑6 and Neutralization by Porcine IL‑6 Antibody. Recombinant Porcine IL‑6 (Catalog # 686-PI) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑6 (12.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF686). The ND50 is typically 0.15-0.6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6

Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1 - 4). Mature porcine IL-6 is 183 amino acids (aa) in length and shares 61%, 42%, and 42% aa sequence identity with human, mouse, and rat IL-6 (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL‑6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8).

References
  1. Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.
  2. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.
  3. Jones, S.A. (2005) J. Immunol. 175:3468.
  4. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.
  5. Mathialagan, N. et al. (1992) Mol. Reprod. Dev. 32:324.
  6. Murakami, M. et al. (1993) Science 260:1808.
  7. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
  8. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
Long Name
Interleukin 6
Entrez Gene IDs
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names
B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Porcine IL-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
Filter your results:

Filter by:

  1. Development and validation of a multiplex fluorescent microsphere immunoassay assay for detection of porcine cytokines
    Authors: SA Hall, SH Ison, C Owles, J Coe, DA Sandercock, AJ Zanella
    MethodsX, 2019-05-17;6(0):1218-1227.
    Species: Porcine
    Sample Types: Tissue Homogenates
    Applications: ELISA Capture
  2. TRAF6 is a novel NS3-interacting protein that inhibits classical swine fever virus replication
    Authors: H Lv, W Dong, Z Cao, X Li, J Wang, G Qian, Q Lv, C Wang, K Guo, Y Zhang
    Sci Rep, 2017-07-27;7(1):6737.
    Species: Porcine
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  3. Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets
    Authors: S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts
    Infect. Immun., 2017-03-23;85(4):.
    Species: Porcine
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  4. Atorvastatin treatment improves survival and effects of implanted mesenchymal stem cells in post-infarct swine hearts.
    Authors: Yang YJ, Qian HY, Huang J, Geng YJ, Gao RL, Dou KF, Yang GS, Li JJ, Shen R, He ZX, Lu MJ, Zhao SH
    Eur. Heart J., 2008-05-02;29(12):1578-90.
    Species: Porcine
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. In vitro induction of mucosa-type dendritic cells by all-trans retinoic acid.
    Authors: Saurer L, McCullough KC, Summerfield A
    J. Immunol., 2007-09-15;179(6):3504-14.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Neutralization
  6. The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia.
    Authors: Nielsen JS, Larsson A, Rix T, Nyboe R, Gjedsted J, Krog J, Ledet T, Tonnesen E
    Intensive Care Med, 2007-04-25;33(6):1085-93.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  7. Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus.
    Authors: Azevedo MS, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV, Saif LJ
    J. Virol., 2006-01-01;80(1):372-82.
    Species: Porcine
    Sample Types: Serum
    Applications: ELISA Development
  8. Long-term effects of polymer-based, slow-release, sirolimus-eluting stents in a porcine coronary model.
    Authors: Carter AJ, Aggarwal M, Kopia GA, Tio F, Tsao PS, Kolata R, Yeung AC, Llanos G, Dooley J, Falotico R
    Cardiovasc. Res., 2004-09-01;63(4):617-24.
    Species: Porcine
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Acute hyperinsulinemia restrains endotoxin-induced systemic inflammatory response: an experimental study in a porcine model.
    Authors: Brix-Christensen V, Andersen SK, Andersen R, Mengel A, Dyhr T, Andersen NT, Schmitz O, Orskov H, Tonnesen E
    Anesthesiology, 2004-04-01;100(4):861-70.
    Species: Porcine
    Sample Types: Plasma
    Applications: ELISA Development

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Porcine IL-6 Antibody

Average Rating: 5 (Based on 2 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Porcine IL-6 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Porcine IL-6 Antibody
By Zoia Levashova on 10/18/2018
Application: MSD assay Sample Tested: Vitreous humor Species: Porcine

After biotinylation, used as a capture reagent according to the manufacturer’s protocol &;Meso Scale Diagnostics LLC;. A standard curve with porcine IL-6 from RnD ;Cat# 686-PI/CF; is shown;2-25,000 pg/ml;.

Other Porcine IL-6 Antibody AF686
Porcine IL-6 Antibody
By Anonymous on 11/08/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Porcine

The polyclonal antibody, AF696, was used as both the capture and detection antibody in a sandwich ELISA. It was specific and sensitive.

ELISA Porcine IL-6 Antibody AF686