Rat Neuropilin-2 Antibody

Catalog #: MAB567R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB567R-100
MAB567R-SP

Detection of Rat Neuropilin‑2 by Western Blot.

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All Neuropilin-2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat Neuropilin-2 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat Neuropilin-2 in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG_2B Clone # 96009R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑2
Gln23-Asp857 (Val809-Asp825 del)
Accession # O35276

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

50 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Rat Neuropilin‑2 by Western Blot. Western blot shows lysates of C6 rat glioma cell line and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat Neuropilin-2 Monoclonal Antibody (Catalog # MAB567R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Neuropilin-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of Neuropilin-2 in C6 Rat Cell Line by Flow Cytometry. C6 Rat glial tumor cell line was stained with Mouse Anti-Rat Neuropilin-2 Monoclonal Antibody (Catalog # MAB567R, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Simple Western

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Detection of Rat Neuropilin‑2 by Simple Western^TM. Simple Western lane view shows lysates of C6 rat glioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Neuropilin‑2 at approximately 138 kDa (as indicated) using 50 µg/mL of Mouse Anti-Rat Neuropilin‑2 Monoclonal Antibody (Catalog # MAB567R). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuropilin-2

Neuropilin-1 (Npn-1, previously known as neuropilin) and Npn-2 (previously known as Npn-1-related molecule) are type I transmembrane proteins that bind members of the class III secreted semaphorin subfamily which are implicated in repulsive axon guidance. The extracellular domain of these proteins is composed of two N-terminal CUB (complement-binding) domains (domains a1 and a2), two domains with homology to coagulation factors V and VIII (domains b1 and b2) and a MAM (meprin) domain (domain c). In the absence of ligands, neuropilins can form homo- and hetero-oligomers via homophilic interactions of their MAM domains. At the amino acid sequence level, Npn-1and Npn-2 share 44% identity. Npn-1 and Npn-2 show different binding specificities for different members of the semaphorin family. The expression patterns of Npn-1 and Npn-2 in developing neurons of the central and peripheral nervous systems are largely, though not completely non-overlapping. Npn‑1 and Npn-2 are also expressed by endothelial and tumor cells and have been shown to be isoform-specific receptors for VEGF₁₆₅. Npn-1 was also reported to bind PlGF-2 and the VEGF-like protein from of virus NZ2.

References

Fujisawa, H. and T. Kitsukawa (1998) Curr. Opin. Neurobiol. 8:587.
Neufeld, G. et al. (1999) FASEB J. 13:9.
Poltorak, Z. et al. (2000) J. Biol. Chem. 275:18040.

Entrez Gene IDs

8828 (Human); 18187 (Mouse); 81527 (Rat)

Alternate Names

MGC126574; neuropilin 2; Neuropilin2; Neuropilin-2; neuropilin-2a(22); neuropilin-2b(0); NP2; NPN 2; NPN2; PRO2714; receptor for VEGF165 and semaphorins class3; Vascular endothelial cell growth factor 165 receptor 2; vascular endothelial growth factor-165 receptor 2; VEGF1265R2; VEGF165R2neuropilin-2a(17)