Proteome Profiler Mouse Apoptosis Array
Proteome Profiler Mouse Apoptosis Array Summary
A membrane-based antibody array for the parallel determination of the relative levels of mouse apoptosis-related proteins. Validated for analyte detection in cell lysates.
Troubleshooting Guide
Key Benefits
- Detects 21 mouse apoptosis-related proteins simultaneously
- Requires no specialized equipment
- Compatible with LI-COR and chemiluminescence detection
Principle of the Assay
The Proteome Profiler Mouse Apoptosis Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of the bound analyte.
Why Use an Antibody Array to Detect Apoptosis-Related Proteins?
Determining the expression of multiple proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.
- Rectangular 8-Well Multi-dish
- 8 Mouse Apoptosis Array nitrocellulose membranes spotted with 21 different antibodies to mouse apoptosis-related proteins
- Array Buffer 1
- Array Buffer 8
- Lysis Buffer 17
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Mouse Apoptosis Array
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
.Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
| Simultaneously detect the levels of these apoptosis-related proteins in a single sample. | ||
| Bad | Fas/TNFRSF6/CD95 | MCL-1 |
| Bcl-2 | HIF-1α | p27/Kip1 |
| Bcl-x | HO-1/HMOX1/HSP32 | p53 |
| Cleaved Caspase-3 | HO-2/HMOX2 | SMAC/Diablo |
| Catalase | HSP27 | TNF RI/TNFRSF1A |
| Claspin | HSP60 | TRAIL R2/TNFRSF10B |
| Cytochrome c | HSP70/HSPA1A | XIAP |
Western blotting antibodies for analytes related to the Mouse Apoptosis Array Kit
| Analyte | Affinity Purified Polyclonal Antibodies (Total) | Monoclonal Antibodies (Total) | Labeled Antibodies (Total) |
| Bad | AF819 | MAB850 | |
| Bcl-2 | AF813 | MAB8272 | |
| MAB810 | |||
| Bcl-x | AF800 | ||
| Cleaved Caspase-3 | MAB835 | ||
| Catalase | AF3398 | ||
| Cytochrome c | MAB897 | ||
| MAB899 | |||
| Fas/TNFRSF6/CD95 | AF435 | BAF435 | |
| HIF-1α | AF1935 | MAB1536 | |
| HO-1/HMOX1/HSP32 | AF3776 | MAB3776 | |
| AF3169 | |||
| HO-2/HMOX2 | AF3170 | MAB3170 | |
| HSP27 | AF1580 | ||
| AF15801 | |||
| HSP60 | AF1800 | MAB1800 | |
| HSP70/HSPA1A | AF1663 | MAB1663 | |
| p27/Kip1 | AF2256 | MAB2256 | |
| p53 | AF1355 | MAB1355 | BAF1355 |
| HAF1355 | |||
| TNF RI/TNFRSF1A | AF-425-PB | MAB430 | BAF425 |
| MAB425 | |||
| TRAIL R2/TNFRSF10B | AF721 | MAB1540 | BAF721 |
| XIAP | AF8221 | MAB822 | BAF8221 |
Specifications
Product Datasheets
Scientific Data
View Larger
Expression of Mouse Apoptosis-related Proteins in Cell Lysate.
Figure 1A: A20 mouse rectal carcinoma cell lysate (650 μg).
Figure 1B: SW/3T3 Swiss albino mouse embryo fi broblast cell lysate (300 μg).
Figure 1C: Ba/F3 mouse lymphocyte cell lysate (400 μg).
Figure 1D: Hepa 1-6 mouse liver epithelial cell lysate (650 μg).
View Larger
Expression of Mouse Apoptosis-related Proteins in Cell Lysate. Figure 2: Lysates from C2C12 mouse muscle myoblast cells were left untreated or treated with 1 μM STS for 3 hours.
Citations for Proteome Profiler Mouse Apoptosis Array
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
RAD21 promotes oncogenesis and lethal progression of prostate cancer
Authors: Su, XA;Stopsack, KH;Schmidt, DR;Ma, D;Li, Z;Scheet, PA;Penney, KL;Lotan, TL;Abida, W;DeArment, EG;Lu, K;Janas, T;Hu, S;Vander Heiden, MG;Loda, M;Boselli, M;Amon, A;Mucci, LA;
Proceedings of the National Academy of Sciences of the United States of America 2024-09-03
-
miR-495 promotes intestinal epithelial cell apoptosis through downregulation of Sphingosine-1-phosphate
Authors: Li, R;Cairns, C;Yu, TX;Jaladanki, R;Dodson, CM;Chung, HK;Xiao, L;Wang, JY;Turner, DJ;
Physiological reports 2024-09-01
-
Oleanolic Acid Acetate Alleviates Cisplatin-Induced Nephrotoxicity via Inhibition of Apoptosis and Necroptosis In Vitro and In Vivo
Authors: Lee, B;Kim, YY;Jeong, S;Lee, SW;Lee, SJ;Rho, MC;Kim, SH;Lee, S;
Toxics 2024-04-18
-
Simultaneous inhibition of PI3K and PAK in preclinical models of neurofibromatosis type 2-related schwannomatosis
Authors: Nagel, A;Huegel, J;Petrilli, A;Rosario, R;Victoria, B;Hardin, HM;Fernandez-Valle, C;
Oncogene 2024-02-09
-
Simultaneous Inhibition of PI3K and PAK in Preclinical Models of Neurofibromatosis Type 2-related Schwannomatosis
Authors: Fernandez-Valle, C;Nagel, A;Huegel, J;Petrilli, A;Rosario, R;Victoria, B;Hardin, H;
Research square 2023-10-16
-
Cardiac Progenitor Cell Exosomal miR-935 Protects against Oxidative Stress
Authors: Aguilar, S;García-Olloqui, P;Amigo-Morán, L;Torán, JL;López, JA;Albericio, G;Abizanda, G;Herrero, D;Vales, Á;Rodríguez-Diaz, S;Higuera, M;García-Martín, R;Vázquez, J;Mora, C;González-Aseguinolaza, G;Prosper, F;Pelacho, B;Bernad, A;
Cells 2023-09-18
-
TRAF2/3 deficient B cells resist DNA damage-induced apoptosis via NF-?B2/XIAP/cIAP2 axis and IAP antagonist sensitizes mutant lymphomas to chemotherapeutic drugs
Authors: Vashisht, M;Ge, H;John, J;McKelvey, HA;Chen, J;Chen, Z;Wang, JH;
Cell death & disease 2023-09-08
-
IkappaB? regulates the development of nonalcoholic fatty liver disease through the attenuation of hepatic steatosis in mice
Authors: H Ishikawa, M Hayakawa, N Baatartsog, N Kakizawa, H Ohto-Ozaki, T Maruyama, K Miura, K Suzuki, T Rikiyama, T Ohmori
Scientific Reports, 2022-07-08;12(1):11634. 2022-07-08
-
MSC Pretreatment for Improved Transplantation Viability Results in Improved Ventricular Function in Infarcted Hearts
Authors: MF Pittenger, S Eghtesad, PG Sanchez, X Liu, Z Wu, L Chen, BP Griffith
International Journal of Molecular Sciences, 2022-01-08;23(2):. 2022-01-08
-
Deficiency of myostatin protects skeletal muscle cells from ischemia reperfusion injury
Authors: C Wallner, M Drysch, M Becerikli, SV Schmidt, S Hahn, JM Wagner, F Reinkemeie, M Dadras, A Sogorski, M von Glinsk, M Lehnhardt, B Behr
Scientific Reports, 2021-06-15;11(1):12572. 2021-06-15
-
The effect of light exposure on the cleavage rate and implantation capacity of preimplantation murine embryos
Authors: Z Bognar, TJ Csabai, E Pallinger, T Balassa, N Farkas, J Schmidt, E Görgey, G Berta, J Szekeres-B, J Bodis
J. Reprod. Immunol., 2019-03-04;132(0):21-28. 2019-03-04
FAQs
No product specific FAQs exist for this product, however you may
View all Proteome Profiler Antibody Array FAQsReviews for Proteome Profiler Mouse Apoptosis Array
There are currently no reviews for this product. Be the first to review Proteome Profiler Mouse Apoptosis Array and earn rewards!
Have you used Proteome Profiler Mouse Apoptosis Array?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
