Human Cytokeratin 19 Antibody

Detection of Cytokeratin 19 by Western Blot. Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line, ZR‑75-1 human breast cancer cell line, DU145 human prostate carcinoma cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB35063) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Cytokeratin 19 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Cytokeratin 19 in RT‑4 Human Cell Line. Cytokeratin 19 was detected in immersion fixed RT‑4 human urinary bladder transitional cell papilloma cell line (positive staining) and THP‑1 human acute monocytic leukemia cell line (negative staining) using Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB35063) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Cytokeratin 19 in MCF‑7 Human Cell Line. Cytokeratin 19 was detected in immersion fixed MCF‑7 human breast cancer cell line (positive staining) and THP‑1 human acute monocytic leukemia cell line (negative staining) using Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB35063) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Cytokeratin 19 in Human prostate. Cytokeratin 19 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Cytokeratin 19 Monoclonal Antibody (Catalog # MAB35063) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to prostate glands. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Cytokeratin-19 in Human MCF-7 cell line by Flow Cytometry. Human MCF-7 breast carcinoma cell line was stained with Mouse Anti-Human Cytokeratin-19 Monoclonal Antibody (Catalog # MAB35063, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by PE-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our protocol for Staining Intracellular Molecules.