Human IGFL-3 Antibody

Catalog #: MAB5970 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5970
MAB5970-SP

Detection of Human IGFL-3 by Western Blot.

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All IGFL-3 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IGFL-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IGFL-3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IGF‑I, rhIGF-II, rhIGFL-1, rhIGFL-4, and recombinant mouse IGFL-3 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 536825

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IGFL-3
Thr25-Pro125
Accession # Q6UXB1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

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Citations (7)

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IGFL-3 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IGFL-3 Monoclonal Antibody (Catalog # MAB5970) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IGFL-3 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFL-3

IGFL-3 (insulin-like growth factor-like 3) is one of four small (~11 kDa), probably secreted human IGFL family members. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family. Mature IGFL-3 is a 101 amino acid (aa) protein that shares 41% aa identity with the sole mouse IGFL. RT-PCR data indicates its expression within the cerebellum.

Long Name

IGF-like Family Member 3

Entrez Gene IDs

388555 (Human)

Alternate Names

IGFL3; IGFL-3; UNQ483