Human Nicotinamide N-Methyltransferase/NNMT Antibody

Catalog #: MAB7736 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7736-100
MAB7736-SP

Detection of Nicotinamide N‑Methyltransferase/NNMT by Western Blot.

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All Nicotinamide N-Methyltransferase/NNMT Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Nicotinamide N-Methyltransferase/NNMT Antibody Summary

Species Reactivity

Human

Specificity

Detects human Nicotinamide N‑Methyltransferase/NNMT in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 954208

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E.coli-derived recombinant human Nicotinamide N‑Methyltransferase/NNMT
Met1-Leu264
Accession # P40261

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Nicotinamide N‑Methyltransferase/NNMT by Western Blot. Western blot shows lysates of human kidney tissue, human lung tissue, A549 human lung carcinoma cell line, and 786-O human renal cell adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Nicotinamide N-Methyltransferase/NNMT Monoclonal Antibody (Catalog # MAB7736) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Nicotinamide N-Methyltransferase/NNMT at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nicotinamide N-Methyltransferase/NNMT

Nicotinamide N-methyltransferase (NNMT) is a 264 aminoacids enzyme that catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. NNMT is found in human fat cells and the liver. Significant up-regulation of NNMT was found pathologies like Parkinson's disease, cancer, atherosclerosis, and liver diseases.

Long Name

Nicotinamide N-Methyltransferase

Entrez Gene IDs

4837 (Human)

Alternate Names

EC 2.1.1.1; Nicotinamide NMethyltransferase; Nicotinamide N-Methyltransferase; NNMT